Dengue virus-induced regulation of the host cell translational machinery

Villas-Bôas, Camila S.A.; Conceição, Thaís M.; Ramı́rez, Jorge; Santoro, Antônio Eduardo Ramires; Poian, Andrea T. Da; Montero-Lomelı́, Mónica

doi:10.1590/s0100-879x2009001100004

Cited by 20 publications

(15 citation statements)

References 37 publications

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“…2B). Many RNA viruses, including encephalomyocarditis virus (EMCV), poliovirus, cricket paralysis virus (CrPV), VSV, Sindbis virus (SINV), Dengue virus (DENV), and reovirus (Gingras et al 1996;Connor and Lyles 2002;Villas-Boas et al 2009;Garrey et al 2010;Mohankumar et al 2011), as well as small DNA viruses such as SV40 (Yu et al 2005), induce the accumulation of hypophosphorylated 4E-BP1, which sequesters the cap-binding subunit eIF4E and prevents eIF4F assembly (Hinnebusch and Lorsch 2012;Roux and Topisirovic 2012). In the case of VSV, this requires the viral M protein, which suppresses Akt-signaling to prevent mTORC1-mediated inactivation of 4E-BP1 (Dunn and Connor 2011).…”

Section: Indirect Effects On Cellular Translation Factorsmentioning

confidence: 99%

Tinkering with Translation: Protein Synthesis in Virus-Infected Cells

Walsh

Mathews

Mohr

2012

Cold Spring Harbor Perspectives in Biology

216

248

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Section: Indirect Effects On Cellular Translation Factorsmentioning

confidence: 99%

Tinkering with Translation: Protein Synthesis in Virus-Infected Cells

Walsh

Mathews

Mohr

2012

Cold Spring Harbor Perspectives in Biology

216

248

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“…It is not known how flavivirus genome RNA translation efficiently competes with cell mRNA translation. Dephosphorylation of p70S6K and 4E-BP1 in infected cells was detected starting at 24 hours after infection (Villas-Boas, Conceicao et al 2009) and cap-independent translation of genome RNA was observed when eIF4E levels were low (Edgil, Polacek et al 2006) suggesting that flavivirus RNA translation may switch to an as yet unknown cap-independent mechanism at later times of infection. Both the 3′ and 5′ NCRs of the flavivirus genome were shown to be required for cap-independent translation.…”

Section: Cell Protein Involvement In Viral Rna Translationmentioning

confidence: 99%

Functions of the 3′ and 5′ genome RNA regions of members of the genus Flavivirus

Brinton

Basu

2015

Virus Research

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The positive sense genomes of members of the genus Flavivirus in the family Flaviviridae are ~11 kb nts in length and have a 5′ type I cap but no 3′ poly A. The 5′ and 3′ terminal regions contain short conserved sequences that are proposed to be repeated remnants of an ancient sequence. However, the functions of most of these conserved sequences have not yet been determined. The terminal regions of the genome also contain multiple conserved RNA structures. Functional data for many of these structures has been obtained. Three sets of complementary 3′ and 5′ terminal region sequences, some of which are located in conserved RNA structures, interact to form a panhandle structure that is required for initiation of minus strand RNA synthesis with the 5′ terminal structure functioning as the promoter. How the switch from the terminal RNA structure base pairing to the long distance RNA-RNA interaction is triggered and regulated is not well understood but evidence suggests involvement of a cell protein binding to three sites on the 3′ terminal RNA structures and a cis-acting metastable 3′ RNA element in the 3′ terminal structure. Cell proteins may also be involved in facilitating exponential replication of nascent genomic RNA within replication vesicles at later times of infection cycle. Other conserved RNA structures and/or sequences in the 5′ and 3′ terminal regions have been proposed to regulate genome translation. Additional functions of the 5′ and 3′ terminal sequences have also been reported.

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“…How flavivirus genome translation efficiently competes with the cell mRNAs, since both have a 5' cap, is not known. The observed upregulation of cap-dependent translation factors prior to 24 h after infection but dephosphorylation of p70S6K and 4E-BP1 at 24 and 48 h [211], as well as the observation of cap-dependent translation of a flavivirus genome when eIF4E was abundant but cap-independent translation when eIF4E levels were low [212], suggests the possibility that the viral RNA may switch to a cap-independent translation mechanism at later times of infection. Both the 3' and 5' UTRs of the flavivirus genome were reported to be required for cap-independent translation.…”

Section: Viral Rna Translation and Replicationmentioning

confidence: 99%

Replication Cycle and Molecular Biology of the West Nile Virus

Brinton

2013

Viruses

121

128

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West Nile virus (WNV) is a member of the genus Flavivirus in the family Flaviviridae. Flaviviruses replicate in the cytoplasm of infected cells and modify the host cell environment. Although much has been learned about virion structure and virion-endosomal membrane fusion, the cell receptor(s) used have not been definitively identified and little is known about the early stages of the virus replication cycle. Members of the genus Flavivirus differ from members of the two other genera of the family by the lack of a genomic internal ribosomal entry sequence and the creation of invaginations in the ER membrane rather than double-membrane vesicles that are used as the sites of exponential genome synthesis. The WNV genome 3' and 5' sequences that form the long distance RNA-RNA interaction required for minus strand initiation have been identified and contact sites on the 5' RNA stem loop for NS5 have been mapped. Structures obtained for many of the viral proteins have provided information relevant to their functions. Viral nonstructural protein interactions are complex and some may occur only in infected cells. Although interactions between many cellular proteins and virus components have been identified, the functions of most of these interactions have not been delineated.

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Dengue virus-induced regulation of the host cell translational machinery

Cited by 20 publications

References 37 publications

Tinkering with Translation: Protein Synthesis in Virus-Infected Cells

Tinkering with Translation: Protein Synthesis in Virus-Infected Cells

Functions of the 3′ and 5′ genome RNA regions of members of the genus Flavivirus

Replication Cycle and Molecular Biology of the West Nile Virus

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