Dengue Detection: Advances in Diagnostic Tools from Conventional Technology to Point of Care

Kabir, Alamgir; Zilouchian, Hussein; Younas, Muhammad Awais; Asghar, Waseem

doi:10.3390/bios11070206

Cited by 46 publications

(47 citation statements)

References 204 publications

(274 reference statements)

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“…Microfluidics is a multipurpose technology that combines several technologies. This also has advantages such as reduced reagent use, little material handling, and a quick test time (Kabir et al, 2021).…”

Section: Detection Of Dengue Virusmentioning

confidence: 99%

New Age Detection of Viruses: The Nano-Biosensors

Shand

Dutta

Rajakumar

et al. 2022

Front. Nanotechnol.

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Viruses and their related diseases have always posed a significant hazard to humans. The current pandemic caused by the Covid-19 (SARS-CoV-2) virus is the latest illustration of what this tiny organism can do to humanity at large, putting everything on the brink of collapse. So it is reasonable that early diagnosis of infection from viruses remains a crucial step to prevent such human suffering. Many traditional methods are already in use for detecting viruses, including molecular approaches, serological methods, direct virus culture methods, and so on. Such traditional methods though are brilliant at some stages but are not devoid of drawbacks. To overcome the limits of conventional procedures, new techniques have been developed which tried to eradicate the demerits of the former procedures. Biosensors have come up with a lot of promises in terms of detecting viruses and diseases connected with them. The development of various types of such biosensors such as Affinity-based nano-biosensors, Nanoisland affinity-based biosensors, Graphene affinity-based biosensors, Nanowires based biosensors, Optical nano biosensors, Fiber optic nano-biosensors, Surface Plasmon Resonance (SPR) based optical nano-biosensors, Total internal reflection fluorescence, Surface-Enhanced Raman Scattering (SERS), Electrochemical nano-biosensors had helped us in the rapid and sensitive detection of viruses. Aid to these nanosensors, viral detection now becomes very sensitive, rapid and cost has come down to a significant low. In this review, an attempt has been made to compile all of the different nano-biosensors and their applications. Due attention is given to the fact that the reader gets the grasp of the concept with much ease.

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Section: Detection Of Dengue Virusmentioning

confidence: 99%

New Age Detection of Viruses: The Nano-Biosensors

Shand

Dutta

Rajakumar

et al. 2022

Front. Nanotechnol.

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“…The dengue virus can be diagnosed by viral RNA detection in reverse transcriptase-polymerase chain reaction (RT-PCR) or non-structural protein 1 (NS1) via enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay (LFIA). − The NS1 antigen is a non-structural glycoprotein, which could reach up to 50 μg/mL in the serum of certain dengue virus-infected patients. As a result, its presence is often used to diagnose the disease .…”

Section: Introductionmentioning

confidence: 99%

Spin-Enhanced Lateral Flow Immunoassay for High-Sensitivity Detection of Nonstructural Protein NS1 Serotypes of the Dengue Virus

Hsiao

Cheng

et al. 2022

Anal. Chem.

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Dengue fever is a global mosquito-borne viral infectious disease that has, in recent years, rapidly spread to almost all regions of the world. Lack of vaccination and directed treatment makes detection at the infection’s early stages extremely important for disease prevention and clinical care. In this paper, we developed a rapid and highly sensitive dengue detection tool using a novel platform of diagnosis, called spin-enhanced lateral flow immunoassay (SELFIA) with a fluorescent nanodiamond (FND) as a reporter. Taking advantage of the unique magneto-optical properties of negatively charged nitrogen-vacancy centers in the FND, the SELFIA platform utilizes alternating electromagnetic fields to modulate signals from FND’s fluorescence to provide sensitive and specific results. With sandwich SELFIA, we could efficiently detect all four dengue non-structural protein (NS1) serotypes (DV1, DV2, DV3, and DV4). The lowest detection concentration of the dengue NS1 antigens varied from 0.1 to 1.3 ng/mL, which is among the lowest limits of detection to date. The FND-based SELFIA technique is up to 500 and 5000 times more sensitive than carbon black and conventional gold nanoparticles, respectively. By using different anti-NS1 antibodies, we could differentiate the NS1 antigen serotypes contained in the tested samples via three simultaneous assays. Proposed SELFIA allows for both qualitative and quantitative differentiation between different NS1 protein serotypes, which will assist in the development of a highly sensitive and specific detection platform for dengue screening that has the potential to detect the disease at its early stages, especially in high-risk and limited-resource areas.

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“…However, antibody responses could be detected only at 3-5 days after the onset of fever, rendering early detection implausible [6,7]. The detection of DENV non-structural protein 1 (NS1) by ELISA or rapid diagnostic test (RDT) has also been widely used, but sensitivity (up to 70% false negative rate) varied among DENV serotypes and manufacturers [8][9][10][11][12], as reviewed by A. Kabir et al [13]. Reverse transcription loopmediated isothermal amplification (RT-LAMP) was also developed as a diagnostic tool for use in areas with limited resources [14,15].…”

Section: Introductionmentioning

confidence: 99%

Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification

Songjaeng

Thiemmeca²,

Mairiang³

et al. 2022

Viruses

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Dengue virus (DENV) infection is a significant global health problem. There are no specific therapeutics or widely available vaccines. Early diagnosis is critical for patient management. Viral RNA detection by multiplex RT-PCR using multiple pairs of primers/probes allowing the simultaneous detection of all four DENV serotypes is commonly used. However, increasing the number of primers in the RT-PCR reaction reduces the sensitivity of detection due to the increased possibility of primer dimer formation. Here, a one tube, singleplex real-time RT-PCR specific to DENV 3′-UTR was developed for the detection and quantification of pan-DENV with no cross reactivity to other flaviviruses. The sensitivity of DENV detection was as high as 96.9% in clinical specimens collected at the first day of hospitalization. Our assay provided equivalent PCR efficiency and RNA quantification among each DENV serotype. The assay’s performance was comparable with previously established real-time RT-PCR targeting coding sequences. Using both assays on the same specimens, our results indicate the presence of defective virus particles in the circulation of patients infected with all serotypes. Dual regions targeting RT-PCR enhanced the sensitivity of viral genome detection especially during the late acute phase when viremia rapidly decline and an incomplete viral genome was clinically evident.

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Dengue Detection: Advances in Diagnostic Tools from Conventional Technology to Point of Care

Cited by 46 publications

References 204 publications

New Age Detection of Viruses: The Nano-Biosensors

New Age Detection of Viruses: The Nano-Biosensors

Spin-Enhanced Lateral Flow Immunoassay for High-Sensitivity Detection of Nonstructural Protein NS1 Serotypes of the Dengue Virus

Development of a Singleplex Real-Time Reverse Transcriptase PCR Assay for Pan-Dengue Virus Detection and Quantification

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