Dengue and Chikungunya among Febrile Outpatients in Kinshasa, Democratic Republic of Congo: a cross-sectional study

Proesmans, Sam; Katshongo, Freddy; Milambu, John; Fungula, Blaise; Mavoko, Hypolite Muhindo; Ahuka‐Mundeke, Steve; Luz, Raquel Inocêncio da; Esbroeck, Marjan Van; Ariën, Kevin K.; Cnops, Lieselotte; Smet, Birgit De; Lutumba, Pascal; Geertruyden, Jean-Pierre Van; Vanlerberghe, Veerle

doi:10.1101/486407

Cited by 3 publications

(4 citation statements)

References 30 publications

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“…Although no epidemics have been reported in the country, our study shows an ongoing arboviral activity in the studied population. The prevalence data on CHIKV was in line with seroprevalence studies conducted in other African countries such as Congo Brazzaville and Democratic Republic of Congo in which an estimate of 34.4% and 24.6% respectively had been reported [37,38]. However, it was far higher than that found in Tanzania [39].…”

Section: Discussionsupporting

confidence: 86%

Sero-prevalence of arthropod-borne viral infections among Lukanga swamp residents in Zambia

et al. 2020

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Introduction The re-emergence of vector borne diseases affecting millions of people in recent years has drawn attention to arboviruses globally. Here, we report on the sero-prevalence of chikungunya virus (CHIKV), dengue virus (DENV), mayaro virus (MAYV) and zika virus (ZIKV) in a swamp community in Zambia. Methods We collected blood and saliva samples from residents of Lukanga swamps in 2016 during a mass-cholera vaccination campaign. Over 10,000 residents were vaccinated with two doses of Shanchol™ during this period. The biological samples were collected prior to vaccination (baseline) and at specified time points after vaccination. We tested a total of 214 baseline stored serum samples for IgG antibodies against NS1 of DENV and ZIKV and E2 of CHIKV and MAYV on ELISA. We defined sero-prevalence as the proportion of participants with optical density (OD) values above a defined cutoff value, determined using a finite mixture model. Results Of the 214 participants, 79 (36.9%; 95% CI 30.5-43.8) were sero-positive for Chikungunya; 23 (10.8%; 95% CI 6.9-15.7) for Zika, 36 (16.8%; 95% CI 12.1-22.5) for Dengue and 42 (19.6%; 95% CI 14.5-25.6) for Mayaro. Older participants were more likely to have Zika virus whilst those involved with fishing activities were at greater risk of contracting Chikungunya virus. Among all the antigens tested, we also found that Chikungunya saliva antibody titres correlated with baseline serum titres (Spearman's correlation coefficient = 0.222; p = 0.03).

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Section: Discussionsupporting

confidence: 86%

Sero-prevalence of arthropod-borne viral infections among Lukanga swamp residents in Zambia

et al. 2020

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“…This compel introduction of arboviral testing in the routine laboratory procedures. However absence of dengue infection in our study population and other African settings can possibly be due to lower OSBPL10 expression pro le in Africans which is protective against viral hemorrhagic fever and dengue shock syndrome (22). Transmission occurs mostly during epidemics.…”

Section: Discussionmentioning

confidence: 66%

“…Further investigation of all cases revealed that 11.7% ( 23) had chikungunya virus while none had dengue virus. For the cases of chikungunya, 78.3% (18) were below 5 years of age, 65.2% ( 15) were females, majority were from Kilimanjaro 95.7% ( 22) and Arusha region 4.3% (1). The clinical features were as follows; nausea/vomit 50.0% (98), cough 48.5% (95), convulsion/comma 34.7%( 68), diarrhea 26.0% (51), joint pain 13.3% (26), sore throat 7.1% ( 14), rashes 4.1%(8)chills 2.6% 5 .…”

Section: Resultsmentioning

confidence: 98%

Chikungunya and dengue virus infection among febrile children in North-Eastern Tanzania: prospective study

Saro¹,

Kajeguka²,

Azizi³

et al. 2020

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Background There are several unknown illnesses including chikungunya and dengue viruses that present with fever in children. Therefore there are many cases that are misdiagnosed. Consequently we performed a study to determine the clinical characteristics of dengue and chikungunya in order to assist clinicians in management. Methods A total of 196 children with history of fever for ≤ 10 days were enrolled prospectively at Kilimanjaro Christian Medical Centre from September 2015 to May 2016. All cases were screened for chikungunya and dengue viruses by PCR as well as other febrile illnesses such as malaria, bacteria and HIV through other diagnostic method. We performed logistic regression to find association between clinical symptoms and chikungunya infection. Results In our study, 21.9% (43/196) of the cases received laboratory investigations and the diagnoses were as follows; malaria only (n = 1, 0.5%), bacterial infections only (n = 4, 2.0%), HIV/AIDS only (n = 37, 18.9%), as well as malaria, meningitis and urinary tract co-infection (n = 1, 0.5%). Further investigation of all cases revealed that 11.7%( 23) had chikungunya virus while none had dengue virus. For the cases of chikungunya, 78.3% (18) were below 5 years of age, 65.2%( 15) were females, majority were from Kilimanjaro 95.7%( 22) and Arusha region 4.3% (1). The clinical features were as follows; nausea/vomit 50.0% (98), cough 48.5% (95), convulsion/comma 34.7%( 68), diarrhea 26.0% (51), joint pain 13.3% (26), sore throat 7.1%( 14), rashes 4.1%(8)chills 2.6%༈5༉. Nevertheless, there was no statistical significant relationship between chikungunya virus and aforementioned symptoms/signs according to logistic regression. Conclusion This study reveals that chikungunya infection is common cause of febrile illnesses; however it does not have treatment. Therefore, we encourage chikungunya infection to be included in routine investigation as these children are receiving inappropriate treatments such as antibiotics and ant malarial which should be avoided in order to minimize over use of drugs and resource wastage.

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“…Probable chikungunya cases were observed in Cité Verte and Ngaliema during the poorly documented chikungunya outbreak in 2012 [27,30,31,34]. Furthermore, antibodies against CHIKV and DENV were reported in approximately 30% of febrile patients from Selembao and Ngaliema, tested in 2005-2006 [29].…”

Section: Study Sitesmentioning

confidence: 99%

Insecticide susceptibility of Aedes (Stegomyia) aegypti (Linnaeus, 1762) and Aedes (Stegomyia) albopictus (Skuse, 1894) in Kinshasa, Democratic Republic of the Congo

Vulu

Ilombe

Vizcaino

et al. 2021

Preprint

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Aedes aegypti and Aedes albopictus are arbovirus vectors of public health concern. Although the Democratic Republic of the Congo (DRC) faces a long-standing risk of Aedes-borne viruses, data on insecticide resistance of Aedes populations are absent. To address this gap, we investigated insecticide susceptibility of Ae. aegypti and Ae. albopictus in areas with a high risk of arbovirus transmission. We also investigated the frequency of knock-down resistance (kdr) mutations in Ae. aegypti. Immature stages of Ae. aegypti and Ae. albopictus were collected from two sites in Kinshasa (Lingwala and Cité Verte) between April and July 2017 and reared to the adult stage. Wild-caught adult Ae. aegypti were collected in 2016 in another site (Ngaliema). Female Ae. aegypti (from Lingwala) and Ae. albopictus (from Cité Verte) were used in WHO tube insecticide susceptibility tests. The F1534C, V1016I and V410L kdr mutations were genotyped in Ae. aegypti from Lingwala and Ngaliema. We observed Ae. aegypti to be susceptible to bendiocarb, propoxur and malathion, suspected resistant to permethrin, and resistant to deltamethrin and DDT. Aedes albopictus was susceptible to bendiocarb, propoxur, malathion and permethrin, suspected resistant to deltamethrin and resistant to DDT. While F1534C and V1016I were not detected, a few Ae. aegypti from Lingwala were heterozygous for the mutation V410L. This study reports for the first time the insecticide resistance status of Aedes spp. and the detection of the kdr mutation V410L in Ae. aegypti in DRC. Given the resistance profile, carbamates and potentially malathion are recommended insecticide options against Ae. aegypti in Kinshasa. It will be important to develop Aedes control strategies based on the resistance patterns of Aedes in Kinshasa.

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Dengue and Chikungunya among Febrile Outpatients in Kinshasa, Democratic Republic of Congo: a cross-sectional study

Abstract: Background

Cited by 3 publications

References 30 publications

Sero-prevalence of arthropod-borne viral infections among Lukanga swamp residents in Zambia

Sero-prevalence of arthropod-borne viral infections among Lukanga swamp residents in Zambia

Chikungunya and dengue virus infection among febrile children in North-Eastern Tanzania: prospective study

Insecticide susceptibility of Aedes (Stegomyia) aegypti (Linnaeus, 1762) and Aedes (Stegomyia) albopictus (Skuse, 1894) in Kinshasa, Democratic Republic of the Congo

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