Dengue‐1 Virus Permissiveness of Established Cell Line Cultures

Shiraki, Kazuko; Hotta, Susumu

doi:10.1111/j.1348-0421.1977.tb00305.x

Cited by 1 publication

(2 citation statements)

References 10 publications

(4 reference statements)

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“…cell susceptibiity to dengue virus infection under experimental conditions allowed us to obtain quantitative results, using a plaque assay, within 3 days for D2 and within 4 days for dengue virus type 1, Hawaii and Mochizuki strains and dengue virus type 4, strain H-241. This assay permits titration of dengue viruses 2 or more days faster than has been reported by other investigators (2,6,10,(17)(18)(19)(20)(21)(22)(23)(24)26). Our results showed that BHK-21 cells can produce dengue virus under a serum-free overlay medium and that the presence of 1 or 2% serum in the overlay medium delayed dengue plaque formation (Tables 3 and 5).…”

Section: Discussionmentioning

confidence: 86%

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Development of dengue virus plaques under serum-free overlay medium

Malewicz¹,

Jenkin²

1979

J Clin Microbiol

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An improved plaque assay for dengue virus was developed utilizing baby hamster kidney (BHK-21) cells initially grown in shaker culture. Different media preparations were tested for uniform and fast formation of BHK-21 cell sheets. Several overlay formulas were tested to develop a rapid plaque assay in 6- and 24-well plastic plates. The best results were obtained utilizing Eagle minimal essential medium (pH 7.2 to 7.4) supplemented with 1 mg of NaHCO3 per ml and 5% newborn calf serum for the formation of cell monolayers after 8 to 24 h of incubation at 37 degrees C. Serum-free Eagle minimal essential medium supplemented with 1% methylcellulose and buffered with 10 mM N-2-hydroxyethyl piperazine-N'-2-ethanesulfonic acid (pH 7.4 to 7.6) was used as an overlay medium. This system allowed for plaque formation after 3 days of incubation of dengue type 2 virus and after 4 days for dengue type 1 and 4 viruses.

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Section: Discussionmentioning

confidence: 86%

“…The Mg2e concentration used by Stim (0.1015 g/ml = 4.23 M) was more than 100x in excess of the physiological Mg2" concentration for LLC-MK2 cells (12). Other authors used about 1 mM Mg2+ with LLC-MK2 cells when plaquing dengue viruses (2,10,17,21).…”

Section: Discussionmentioning

confidence: 99%

Development of dengue virus plaques under serum-free overlay medium

Malewicz¹,

Jenkin²

1979

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

Dengue‐1 Virus Permissiveness of Established Cell Line Cultures

Cited by 1 publication

References 10 publications

Development of dengue virus plaques under serum-free overlay medium

Development of dengue virus plaques under serum-free overlay medium

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