Dendrobium officinale Regulates Fatty Acid Metabolism to Ameliorate Liver Lipid Accumulation in NAFLD Mice

Abstract

Dendrobium officinale (DOF) is a traditional Chinese edible and officinal plant. Ultrafine DOF powder (DOFP) can regulate lipids and histopathology in the liver, but the underlying mechanisms of hepatic fatty acid (FA) metabolism, which is generally correlated with the development of nonalcoholic fatty liver disease (NAFLD), remain unclear. The purpose of the present study was to investigate whether DOFP treatment alters hepatic FA metabolism in NAFLD mice by using multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) and analyse the underlying mechanisms. A 3-week DOFP treatment prevented lipid deposition and improved hepatic histopathology in NAFLD mice after withdrawal from the high-sucrose, high-fat (HSHF) diet, and it decreased triglyceride and FA content in the liver. Furthermore, the C16 : 0/C14 : 0 and C18 : 1/18 : 0 ratios in FAs were significantly decreased in the DOFP treatment group, and the C20 : 4/C20 : 3 and C22 : 4/C22 : 3 ratios were increased, and saturated FA was inhibited. Additionally, DOFP treatment significantly increased the content of two FA β-oxidation-related proteins (carnitine palmitoyltransferase 1-α and acyl-coenzyme A oxidase 1). It also decreased the content of a FA synthesis-related protein (fatty acid synthase), a FA desaturation-related protein (stearoyl-coenzyme A desaturase-1), and a FA uptake-related protein (fatty acid transport protein 2). Moreover, DOFP treatment improved dysregulated levels of major phospholipids in the livers of model mice. The results of this study confirm that DOFP treatment in NAFLD mice has liver recovery effects by regulating FA metabolism.

Ethanol Extract of Liriope platyphylla Root Attenuates Non-Alcoholic Fatty Liver Disease in High-Fat Diet-Induced Obese Mice via Regulation of Lipogenesis and Lipid Uptake

“…We analyzed the protein expression levels of CPT1 and ACOX1 to evaluate whether the LPE treatment increases fatty acid β-oxidation. Studies show that CPT1 and ACOX1 induce β-oxidation [ 30 , 31 ]. CPT1 and ACOX1 protein expression levels were not significantly changed in the LPE administration groups compared to the HFD group ( Figure 4 E,F).…”

“…CPT1 is an enzyme that transports FFA into mitochondria for oxidation to produce acetyl-CoA, which is subsequently used to generate ATP via the tricarboxylic acid cycle and electron transport chain [ 30 ]. ACOX1, along with peroxidase, is an initiating enzyme in the β-oxidation system [ 31 ]. When we investigated the expressions of ACOX1 and CPT1 in liver tissue, we found that LPE treatment did not affect the expression of these proteins ( Figure 4 E,F).…”

Benefit Effect of Dendrobium officinale Ultrafine Powder on DSS-Induced Ulcerative Colitis Rats by Improving Colon Mucosal Barrier

“…Our previous literature showed that the total polysaccharide content (47.96%, w/w) and monosaccharide contents (consisted of D-mannose, glucose, galactose, and arabinose at 42.2%, 12.3%, 0.4%, and 0.4%, w/w) were detected in DOFP [18]. e total flavonoid content (3.78 ± 0.48%) and the content of naringenin (0.3601 ± 0.014 mg/g) in DOFP were detected in a sodium hydroxide-aluminum sulfate method and high-performance liquid chromatograph (HPLC) system (1260, Agilent, Germany), respectively, as described in our previous report [19]. Modern pharmacological studies have shown that polysaccharides have anti-inflammatory, antioxidant, antitumor, and other pharmacological effects, enhance immunity, and regulate intestinal flora [24].…”

“…After ultrafine pulverization, the particle size of a drug can reach 1-10 μm as an ultrafine powder [17]. e powder has the advantages of convenient transportation and storage, excellent water solubility, low loss of functional components, high utilization rate, and beneficial dietary fiber [18,19].…”

Polysaccharide, the Active Component of Dendrobium officinale, Ameliorates Metabolic Hypertension in Rats via Regulating Intestinal Flora-SCFAs-Vascular Axis

“…We also reported that Dendrobium officinale ultrafine powder (DOFP) could improve the intestinal flora and increase the SCFA level in feces and serum, thereby activating the SCFA-GPCR43/41 pathway, improving endothelial function, and lowering BP in rats with MH, which provides new insights on the mechanism of Dendrobium officinale in the treatment of MH ( Li et al, 2021 ). Polysaccharides, as the main medicinal ingredient of Dendrobium officinale , have gained increased research interest recently ( Lei et al, 2019 ; Zhang et al, 2020 ; Lei et al, 2021 ). However, it is not clear whether Dendrobium officinale polysaccharide (DOPS) can also improve the intestinal flora and SCFAs and then activate the intestinal SCFA-GPCR43/41 pathway, which can improve vascular function and lower the BP.…”