Dendrobium officinale Polysaccharides Inhibit 1-Methyl-2-Nitro-1-Nitrosoguanidine Induced Precancerous Lesions of Gastric Cancer in Rats through Regulating Wnt/β-Catenin Pathway and Altering Serum Endogenous Metabolites

Abstract

Dendrobium officinale is a herb in traditional Chinese medicine where D. officinale polysaccharides (DOP) are the main active ingredient. This study aimed at evaluating DOP efficiency at inhibiting 1-Methyl-2-nitro-1-nitrosoguanidine (MNNG) induced precancerous lesions of gastric cancer (PLGC) in rats through the Wnt/b-catenin pathway and analyzing the variations of serum endogenous metabolites. PLGC was established in male Sprague-Dawley (SD) rats by administering 150 μg/mL MNNG in drinking water for 7 months and giving 0.1 mL of 10% NaCl once weekly during the initial 20 weeks. Treatment with DOP inhibited the progress of PLGC through decreasing the expression of β-catenin by immunohistochemical analysis. The futher study indicated DOP downregulated gene expression of Wnt2β, Gsk3β, PCNA, CyclinD1, and β-catenin, as well as protein expression of Wnt2β, PCNA, and β-catenin. On the other hand, there were nine endogenous metabolites identified after the DOP treatment. Among these, the most significant one is betaine because of its strong antioxidant activity, leading to an anti-tumor effect. DOP can inhibit MNNG-induced PLGC models via regulating Wnt/β-catenin pathway and by changing endogenous metabolites.

Traditional Uses, Phytochemistry, Pharmacology, and Quality Control of Dendrobium officinale Kimura et. Migo

“…Besides, it is usually prescribed as one of the ingredients of herbal formula, such as "Tin maple crystal" (Tiepifengdoujing: D. officinale and American ginseng), for regulating the gastrointestinal tract in traditional and contemporary clinical practice (Qin et al, 2019). According to currently available phytochemical investigations, the bioactive chemical components of D. officinale mainly consist of polysaccharides (Zhao et al, 2019), bibenzyls (Zhao et al, 2020), phenanthrenes (Liu et al, 2019), flavonoids (Xing et al, 2018), and alkaloids (Chu et al, 2019). In contrast, according to the literature, only few of these compounds have been evaluated in bioactivity assays (Zhao et al, 2018a;Liu et al, 2018;Xing et al, 2018;Chu et al, 2019;Liu et al, 2019;Lee et al, 2020;Zhao et al, 2020).…”

Complementary and alternative therapies for precancerous lesions of gastric cancer

“…With the guidance of TCM theory, TCM can intervene at multiple targets and levels to treat PLGC by protecting gastric mucosa, regulating immunity, affecting cell proliferation and differentiation, and anti-oxidation. [ 24 , 25 ] Moreover, PLGC has gained more and more international recognition by treating it with TCM. [ 26 ] Acupuncture and moxibustion have the characteristics of safety, simplicity, low cost and few side effects, and can significantly improve the clinical symptoms of patients.…”

Comparative analysis of transcriptome and metabolome uncovers the metabolic differences between Dendrobium officinale protocorms and mature stems

“…Dendrobium officinale, also known as Tiepi Shihu in Chinese, is a well-known herb in Traditional Chinese Medicine and has been used for more than 2,000 years. Furthermore, this important species has been listed as a health food by the National Health Commission of the People's Republic of China (He et al 2017;Chen et al 2018;Zhao et al 2019). In the current study, we used a comprehensive approach to examine differences in composition and gene regulation of polysaccharides and flavonoids between DOPs and Mat; in addition, we identified several genes related to the biosynthesis of these compounds using transcriptome analysis.…”