Dendrobium candidum polysaccharide reduce atopic dermatitis symptoms and modulate gut microbiota in DNFB-induced AD-like mice

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease with a high prevalence worldwide. Increasing evidence suggests that the gut microbiota plays an important role in the pathogenesis of AD. In this study, we sought to verify the effect of Dendrobium candidum polysaccharides (DCP) on AD induced by 2,4-Dinitrofluorobenzene (DNFB) in Balb/c mice regarding its impact on the intestinal microbiome. We found that 2-week oral administration of DCP improved AD-like symptoms and histological damage of skin, reduced mast cell infiltration, down-regulated the level of serum total IgE and the expression of pro-inflammatory cytokines such as TNF-α, IFN-γ, IL-4 and IL-6, and increased the expression level of anti-inflammatory cytokine IL-10. The beneficial effect of DCP was attributed to the restoration of the intestinal microbiome composition and the unbalance of the intestinal homeostasis. Our results indicated that DCP might be used as a promising novel microbiota-modulating agent for the treatment of AD.

Therapy and mechanism of natural polysaccharides for atopic dermatitis treatment

“…Tregs can prevent the polarisation of Th2 cells by secreting inhibitory cytokines including IL-10, IL-35, and TNF-β, while AD is generally considered a Th2-driven disease [149] Natural polysaccharides have a function of immunostimulating effects and can increase the proportion of Tregs [150]. Polysaccharides from Angelica gigas Nakai (ISAg) induced the polarization of CD4 + T cells toward the acquisition of the Th1 phenotype and the expansion of Treg in Yet40 B6 mice, which suggests that ISAg may be effective for AD therapy [88]. Fucus vesiculosus polysaccharides have demonstrated immune-stimulating effects, leading to an increase in Tregs [100].…”

“…Inhibition of pathogenic colonization of S. aureus is an important approach for polysaccharides in regulating the skin microbiome in the context of AD. Xyloglucan-containing products have been shown to inhibit the colonization of S. aureus and reduce its adhesion and penetration on the skin of infected mice [88].…”

Sp1‐mediated miR‐193b suppresses atopic dermatitis by regulating HMGB1

“…A total of 18 female BALB/c mice (6-week-old) were purchased from SLACOM, and AD was induced by 2,4-dinitrofluorobenzene (DNFB) treatment as previously described. 21 Briefly, 1% DNFB (Macklin) was dissolved in an acetone/olive oil (1:3) solution. Mice were randomized into three groups (n = 6/group): control, AD, and AD + miR-193b groups.…”

From gut to skin: exploring the potential of natural products targeting microorganisms for atopic dermatitis treatment

“…158 In a study on herbal monomers, the oral Dendrobium candidum polysaccharides ( DCP ) treatment restores the relative abundance of Desulfovibrio and Blautia in DNFB-induced AD-like mice, down to near that of healthy mice. 157 Regarding the herbal compound, Huang-Lian-Jie-Du Decoction and the water-soluble-fraction could markedly alleviate AD-like symptoms induced by DNCB and MC903 in mice, possibly improving the epidermal barrier function and inhibiting skin inflammation via suppressing the activation of NF-κB and MAPKs pathways and regulation of the gut microbiota dysbiosis. 164 Chun et al demonstrated that Sihocheonggan-Tang (SHCGT) ameliorates DNCB-induced AD symptoms in mice by modulating the immune response and altering the gut microbiome composition.…”