Dendrobium candidum inhibits MCF-7 cells proliferation by inducing cell cycle arrest at G2/M phase and regulating key biomarkers

Abstract

BackgroundBreast cancer is one of the most frequently occurring cancers in women. In recent years, Dendrobium candidum has played a part in antihyperthyroidism and anticancer drugs. This study aims to examine the antitumor effect of D. candidum on breast cancer.MethodsHuman breast cancer cell line MCF-7 and normal breast epithelial cell line MCF10A were used to observe the effects of D. candidum treatment on human breast cancer. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was employed to examine the cell proliferation of the MCF-7 and MCF10A cells. Western blot analysis and reverse transcription polymerase chain reaction were used to detect the key molecules and biomarkers in breast cancer pathology. Cell cycle was analyzed by using Becton Dickinson FACScan cytofluorometer.ResultsThe results indicated that D. candidum significantly decreased cell viability at different concentrations compared to the control group (P<0.05). D. candidum-treated MCF-7 cells in the G2/M phase was significantly increased compared to the control group (P<0.05). The messenger RNA levels of estrogen receptor alpha, IGFBP2, IGFBP4, and GATA3 were significantly decreased, and the messenger RNA and protein levels of ELF5, p53, p21, p18, CDH1, CDH2, and p12 were significantly increased, compared to the control group (P<0.05). The protein levels of estrogen receptor alpha, PGR, GATA3, and Ki67 were significantly decreased and the protein levels of p53 and ELF5 were significantly increased compared to the control group (P<0.05). The general apoptosis biomarker, Bcl-2, was significantly decreased and the Bax was significantly increased compared to the control group (P<0.05). In contrast to that in MCF-7, D. candidum does not affect cell proliferation at any concentration and any time points in normal breast epithelial cells, MCF10A cells.ConclusionD. candidum could decrease the cell viability of MCF-7 cells by inducing cell cycle arrest at the G2/M phase and regulating the key biomarkers in breast cancer cells.

Antioxidant and cytotoxic activities of Dendrobium moniliforme extracts and the detection of related compounds by GC-MS

“…The several phenol derivatives detected in these extracts arrest the cell cycle and induce apoptosis through morphological changes like cell shrinkage, chromatin condensation, and nuclear fragmentation [ 28 , 55 , 56 ], thereby serving as an important defense mechanism preventing the proliferation of cancer cells. Like other studies of the anticancer activities of Dendrobium species [ 21 – 27 ], this study too, found that phenol derivatives induces the apoptosis of cancer cells. Phenol derivatives of extracts do exhibit both antioxidant and anticancer effects [ 2 – 4 , 57 ].…”

“…The natural antioxidant-rich compounds of D. moniliforme engage in many biological activities, including promoting the production of body fluids, serving neuroprotective, immunomodulatory and antioxidant functions [ 2 , 20 ]. D. candidum has previously been shown to have in vitro anticancer effects on human carcinoma cells [ 21 – 27 ]. Indeed, it is often the case that bioactive agents of folk medicine help prevent the development of cancer by inducing apoptosis [ 28 ].…”

Dendrobium officinale Orchid Extract Prevents Ovariectomy-Induced Osteoporosis in Vivo and Inhibits RANKL-Induced Osteoclast Differentiation in Vitro

“…It is encouraging that natural products from medicinal plants have been shown to be valuable sources for the development of novel drugs against a range of diseases ( Niu et al, 2012 ; Sacco et al, 2013 ). One example is the orchid Dendrobium officinale ( Zhao et al, 2014 ), a traditional Chinese high-value medicinal herb that is widely used across Asia ( Zhao et al, 2014 ; Sun et al, 2016 ). This plant contains polysaccharides, stillnoids, alkaloids, amino acids, and trace elements and is known to exert a number of positive effects on human health ( Li et al, 2010 ; Li L. et al, 2011 ), including anti-tumor, anti-oxidative, anti-chronic inflammation, anti-obesity, anti-hypertensive, hypoglycemic, neuron protection, and immune system modulation activities ( Xiao et al, 2011 ; Zhao et al, 2014 ; Sun et al, 2016 ; Lee et al, 2017 ; Wang Q. et al, 2017 ).…”

MiR-219-5p suppresses cell proliferation and cell cycle progression in esophageal squamous cell carcinoma by targeting CCNA2

“…Lin et al [32] confirmed that depletion of eIF-3d reduces non-small cell lung cancer cell proliferation via enhancement of cell cycle arrest in the G2/M phase. Sun et al [33] proved that the dendrobium candium could suppress the proliferation of MCF-7 cells by increasing G2/M arrest. Here, we suggest that miR-219-5p suppressed ESCC cell proliferation and induced G2/M phase arrest by directly downregulating CCNA2 expression.…”