Dendrobium candidum extract increases the expression of aquaporin-5 in labial glands from patients with Sjögren's syndrome

Lin, Xiao; Ng, Tzi Bun; Feng, Yibin; Tong, Yao; Wong, Jack Ho; Yao, Ren-Min; Li, Lei; Mo, Feizhi; Xiao, Yin; Shaw, Pang‐Chui; Li, Zemin; Sze, Stephen Cho Wing; Zhang, Kalin Yanbo

doi:10.1016/j.phymed.2010.05.002

Cited by 48 publications

(29 citation statements)

References 12 publications

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“…However, there is lack of investigation on the pharmacological function of DP. Our previous work demonstrated the efficacy of DP on both patients and mice model of Sjögren's syndrome [5,13]. In the present study, we expand these observations to examine the underlying mechanism that DP protected the SG from destruction in the experimental SS mice model.…”

Section: Discussionmentioning

confidence: 61%

“…Our previous study showed the protective effect of DP on patients with SS [5], and further, we proved its efficacy on an experimental SS mice model [13]. In both of the studies we found DP could restore the saliva secretion.…”

Section: Introductionmentioning

confidence: 51%

“…It is mainly distributed in the tropical and subtropical areas such as Anhui, Zhejiang, Fujian, Guangxi, Sichuan and Yunnan provinces of China at an elevation of about 1600 m on the mountain [4]. For its clinical use, DO has been applied for maintaining tonicity of stomach and promoting the body fluid production [5]. Polysaccharide, as the major active constituent and rich in Dendrobium species [6], is recently reported to be positive in the immunomodulation [7].…”

Section: Introductionmentioning

confidence: 99%

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Dendrobium officinale polysaccharides ameliorate the abnormality of aquaporin 5, pro-inflammatory cytokines and inhibit apoptosis in the experimental Sjögren's syndrome mice

Lin

Shaw

Sze

et al. 2011

International Immunopharmacology

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Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 51%

Section: Introductionmentioning

confidence: 99%

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Dendrobium officinale polysaccharides ameliorate the abnormality of aquaporin 5, pro-inflammatory cytokines and inhibit apoptosis in the experimental Sjögren's syndrome mice

Lin

Shaw

Sze

et al. 2011

International Immunopharmacology

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“…(Orchidaceae) is one of most valuable medicinal plants widely used in traditional Chinese medicine for maintaining tonicity of the stomach and promoting body fluid production (Li et al 2009). Phytochemical research has demonstrated various active constituents from the herb, such as alkaloids, fluorenones, sesquiterpenoids, bibenzyls, phenanthrenes, and polysaccharides, which exhibit diverse pharmacological effects, including antioxidant, immune stimulating, anti-tumor, and anti-mutagenic activities (Li et al 2009;Luo et al 2010;Xiao et al 2011). Given these characters of D. candidum, there is an urgent need for its functional genomics research from multiple aspects, like plant growth and development regulation, adaptation to abiotic and biotic stresses, etc.…”

Section: Introductionmentioning

confidence: 99%

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

et al. 2012

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AbstractCyclin-dependent kinases (CDKs) play an essential role in cell cycle regulation during the embryonic and postembryonic development of organisms. To better understand the molecular mechanisms of CDKs involved in embryogenesis regulation in the endangered medicinal plant Dendrobium candidum Wall. ex Lindl., a 1229-bp full-length cDNA of an A-type CDK gene, Denca;CDKA;1, was identified using 3′ rapid amplification of cDNA end (RACE) PCR. Denca;CDKA;1 was predicted to encode a 294 amino acid residue-long protein of 33.76 kDa with an isoelectric point of 7.72. The deduced Denca;CDKA;1 protein contained a conserved serine/threonine-protein kinase domain (S-TKc) and a canonical cyclinbinding “PSTAIRE” motif. Multiple sequence alignment indicated that members of CDKA family from various plants exhibited a high degree of sequence identity ranging from 82% to 93%. A neighbor-joining phylogenetic tree showed that Denca;CDKA;1 was clustered into the plant group and was distant from the animal and fungal groups. The modeled three-dimensional structure of Denca;CDKA;1 exhibited the similar functional structure of a fold consisting of β-sheets and α-helices joined by discontinuous random coils forming two relatively independent lobes. Quantitative real-time PCR analysis revealed that Denca;CDKA;1 transcripts were the most abundant in protocorm-like bodies with 4.76 fold, followed by that in roots (4.19 fold), seeds (2.57 fold), and stems (1.57 fold). This study characterized the novel Denca;CDKA;1 gene from D. candidum for the first time and the results will be useful for further functional determination of the gene.

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“…candidate for modern drug development because it enhances immunity, exhibits antitumor activity, and other regulatory roles (Li et al, 2009;Luo et al, 2010;Xiao et al, 2011;Xing, 2013;Bian et al, 2002). The production of D. candidum in Yunnan was estimated to be lower than 1000 kg in 1998, which cannot meet the market (Huang et al, 1996), the development of D. candidum cultivation, is therefore necessary.…”

Section: Introductionmentioning

confidence: 99%

Establishment and optimization of high efficiency embryogenic callus induction system in Dendrobium candidum

Refish¹,

Wang²,

Fu³

et al. 2016

Afr. J. Plant Sci.

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The wild resource of Dendrobium candidum, a well-known epiphytic orchid, is limited. Plant cell culture technology is a promising alternative for production of high-value secondary metabolites .In this research, factors affecting the induction, maintenance, and multiplication of callus from protocorm-like bodies (PLBs) of D. candidum were systematically studied, and a technical callus inducing system with 100% rate was established which higher than previously reported values. The result showed that 2,4-dichlorophenoxy acetic acid(2,4-D) and 1-naphthalene acetic acid (NAA) could of benefit to the callus induction. The optimized medium for callus induction was MS medium containing 0.5 mg/L 2,4-D and 0.25 NAA or 0.5 mg/L kinetin (KT). The callus clump enrichment method was used for directional screening and cell adaptation on MS medium supplied with plant growth regulators (PGR) such as 5 mg/L NAA and 0.5 mg/L 6-benzyladenine (6-BA) or KT for 30 days per cycle. After 6 to 9 months, D. candidum cell lines were obtained and the histological analysis showed that the main differences between callus and PLBs were meristematic cell content and internal cell differentiation degree, and the meristematic cells distributed in the external strip were contributed to the formation of callus. At last, the amount of polysaccharides and total amino acids were compared between the obtained cell lines, tissue culture seedlings and PLBs. The results showed that polysaccharides was 24.67% in callus and higher than that in tissue culture seedlings and PLBs. The amount of total amino acids was 5.94% in callus and higher than that in tissue culture seedlings. So the callus is considered a good choice for the expanding of D. candidum medicine sources.

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Dendrobium candidum extract increases the expression of aquaporin-5 in labial glands from patients with Sjögren's syndrome

Cited by 48 publications

References 12 publications

Dendrobium officinale polysaccharides ameliorate the abnormality of aquaporin 5, pro-inflammatory cytokines and inhibit apoptosis in the experimental Sjögren's syndrome mice

Dendrobium officinale polysaccharides ameliorate the abnormality of aquaporin 5, pro-inflammatory cytokines and inhibit apoptosis in the experimental Sjögren's syndrome mice

Characterization of an A-type cyclin-dependent kinase gene from Dendrobium candidum

Establishment and optimization of high efficiency embryogenic callus induction system in Dendrobium candidum

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