Dendritic colocalisation of serotonin1B receptors and the glutamate NMDA receptor subunit NR1 within the hippocampal dentate gyrus: An ultrastructural study

Peddie, Christopher J.; Davies, Heather A.; Colyer, Frances; Stewart, Michael G.; Rodrı́guez, José J.

doi:10.1016/j.jchemneu.2008.05.001

Cited by 27 publications

(20 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…45 Moreover, recent data also indicate the presence of 5-HT 1B Rs on dendrites of hippocampal neurons, particularly granular cells of the dentate gyrus. 46 The current data imply that hippocampal 5-HT 1B Rs are of at least two types; 5-HT 1B Rs regulated by p11 and suppressing emotional memory processing, and other 5-HT 1B Rs that are independent of p11 and enhance emotional memory (Fig 5). …”

Section: Discussionmentioning

confidence: 56%

Bidirectional regulation of emotional memory by 5-HT1B receptors involves hippocampal p11

et al. 2012

View full text Add to dashboard Cite

Cognitive impairments are common in depression and involve dysfunctional serotonin neurotransmission. The 5-HT1B receptor (5-HT1BR) regulates serotonin transmission, via presynaptic receptors, but can also affect transmitter release at heterosynaptic sites. This study aimed at investigating the roles of the 5-HT1BR, and its adapter protein p11, in emotional memory and object recognition memory processes by the use of p11 knockout (p11KO) mice, a genetic model for aspects of depression-related states. 5-HT1BR agonist treatment induced an impairing effect on emotional memory in wild type (WT) mice. In comparison, p11KO mice displayed reduced long-term emotional memory performance. Unexpectedly, 5-HT1BR agonist stimulation enhanced memory in p11KO mice, and this atypical switch was reversed after hippocampal adeno-associated virus mediated gene transfer of p11. Notably, 5-HT1BR stimulation increased glutamatergic neurotransmission in the hippocampus in p11KO mice, but not in WT mice, as measured by both pre- and postsynaptic criteria. Magnetic resonance spectroscopy demonstrated global hippocampal reductions of inhibitory GABA, which may contribute to the memory enhancement and potentiation of pre- and post-synaptic measures of glutamate transmission by a 5-HT1BR agonist in p11KO mice. It is concluded that the level of hippocampal p11 determines the directionality of 5-HT1BR action on emotional memory processing and modulates hippocampal functionality. These results emphasize the importance of using relevant disease models when evaluating the role of serotonin neurotransmission in cognitive deficits related to psychiatric disorders.

show abstract

Section: Discussionmentioning

confidence: 56%

Bidirectional regulation of emotional memory by 5-HT1B receptors involves hippocampal p11

et al. 2012

View full text Add to dashboard Cite

show abstract

“…14, 15 This effect is mediated by activation of 5-HT 1A and 5-HT 1B receptors that are highly expressed in the hippocampal formation. 30, 31 In support of the above hypothesis, a recent study by Verdurand et al 16 reported enhanced 5-HT neurotransmission and 5-HT 1A receptors expression following intrahippocampal infusion of A β . Increased expression of hippocampal 5-HT 1A receptors was also reported in patients with mild cognitive impairment who exhibit greater risk of developing AD.…”

Section: Discussionmentioning

confidence: 83%

“…27, 30 The sections were then washed in 0.1 M PB for 10 min followed by sequential dehydration through replacement with graded ethanol series (30, 50, 70 and 95%, 5 min each). The sections were further dehydrated using 100% ethanol (10 min), propylene oxide (2 × 15 min) and propylene oxide/EPON (1 : 1, Agar Scientific Ltd, Stanstead, UK) overnight at room temperature rotating.…”

Section: Methodsmentioning

confidence: 99%

“…The propylene oxide/EPON was replaced with 100% EPON and rotated for further 2 h. The sections were then flat embedded between sheets of Aclar fluo halocarbon film (Agar Scientific Ltd). 27, 30 Polymerisation was carried out by incubating at 60 °C overnight. Following polymerisation, the region of interest (CA1 subfield of the hippocampus) was selected and micro-dissected from the flat-embedded tissue and mounted on the tip of EPON blocks 27 (Figure 1), keeping the same levels and coordinates between the different animals.…”

Section: Methodsmentioning

confidence: 99%

“…The labelled profiles were classified as somata, dendrites, dendritic spines, unmyelinated axons, axon terminals and glia, according to their morphological features as described previously 27, 30 and defined by Peters et al 48 Synapses were defined as either symmetric when having thin pre- and postsynaptic densities or asymmetric when having a thin pre- and a thick postsynaptic membrane specialisation. 27 Perforated synapses were defined as those asymmetric synapses with a notable discontinuity (>50 nm) in the electron density of the postsynaptic junction as previously described.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Increased hippocampal CA1 density of serotonergic terminals in a triple transgenic mouse model of Alzheimer's disease: an ultrastructural study

et al. 2011

View full text Add to dashboard Cite

Alzheimer's disease (AD) is a neurodegenerative pathology that deteriorates mnesic functions and associated brain regions including the hippocampus. Serotonin (5-HT) has an important role in cognition. We recently demonstrated an increase in 5-HT transporter (SERT) fibre density in the hippocampal CA1 in an AD triple transgenic mouse model (3xTg-AD). Here, we analyse the ultrastructural localisation, distribution and numerical density (Nv) of hippocampal SERT axons (SERT-Ax) and terminals (SERT-Te) and their relationship with SERT fibre sprouting and altered synaptic Nv in 3xTg-AD compared with non-transgenic control mice. 3xTg-AD animals showed a significant increase in SERT-Te Nv in CA1 at both, 3 (95%) and 18 months of age (144%), being restricted to the CA1 stratum moleculare (S. Mol; 227% at 3 and 180% at 18 months). 3xTg-AD animals also exhibit reduced Nv of perforated axospinous synapses (PS) in CA1 S. Mol (56% at 3 and 52% at 18 months). No changes were observed in the Nv of symmetric and asymmetrical synapses or SERT-Ax. Our results suggest that concomitant SERT-Te Nv increase and PS reduction in 3xTg-AD mice may act as a compensatory mechanism maintaining synaptic efficacy as a response to the AD cognitive impairment.

show abstract

Neuromodulation of Hippocampal Cells and Circuits

Lawrence¹,

Cobb²

2018

Springer Series in Computational Neuroscience

View full text Add to dashboard Cite

Dendritic colocalisation of serotonin1B receptors and the glutamate NMDA receptor subunit NR1 within the hippocampal dentate gyrus: An ultrastructural study

Cited by 27 publications

References 89 publications

Bidirectional regulation of emotional memory by 5-HT1B receptors involves hippocampal p11

Bidirectional regulation of emotional memory by 5-HT1B receptors involves hippocampal p11

Increased hippocampal CA1 density of serotonergic terminals in a triple transgenic mouse model of Alzheimer's disease: an ultrastructural study

Neuromodulation of Hippocampal Cells and Circuits

Contact Info

Product

Resources

About