Dendritic cells: from ontogenetic orphans to myelomonocytic descendants

Peters, J. H.; Gieseler, Robert K.; Thiele, Bernhard; Steinbach, Falko

doi:10.1016/0167-5699(96)80544-5

Cited by 306 publications

(186 citation statements)

References 46 publications

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“…Interestingly, a recent report suggests that APC generated from human PBMC under the influence of GM-CSF þ IL-4, are still not fully mature but require monocyte-conditioned medium to complete the process [42]. Alternatively, our data may exemplify divergence in the developmental pathway of myelomonocytic cells [43]. This possibility is supported by reports of enhanced MLR to GM/IL-4 BMAPC.…”

Section: Discussionsupporting

confidence: 72%

Priming to Mycobacterial Antigen In Vivo Using Antigen‐Pulsed Antigen Presenting Cells Generated In Vitro is Influenced by the Dose and Presence of IL‐4 in APC Cultures

Dillon

Hart

Abernethy³

et al. 1997

Scand J Immunol

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Antigen presenting cells (APC) similar to immature dendritic cells can be generated in vitro from bone marrow precursors. The authors have compared the yield, the phenotype and the function of murine bone marrow cells cultured for 7 or 11 days in either granulocyte macrophage colony stimulating factor alone (GM BMAPC) or in combination with interleukin‐4 (GM/IL‐4 BMAPC). The results showed that GM/IL‐4 BMAPC expressed the highest levels of MHC Class 2 molecules, CD86 /B7‐2 and CD80/B7‐1 co‐stimulatory molecules and the lowest levels of F4/80 macrophage marker. However, when these APC were pulsed with BCG culture filtrate antigen or PPD they were not correspondingly more effective at stimulating activated T lymphocytes in vitro or priming naive T lymphocytes in vivo. Also, in contrast to GM BMAPC, high backgrounds recorded following injections of GM/IL‐4 BMAPC without antigen were not consistently reduced by lowering the dose and irradiating the cells prior to administration. The authors conclude that the degree of maturity of BMAPC varies with culture conditions and that this may be an important consideration where BMAPC are to be used in vivo in immunotherapeutic regimens.

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Section: Discussionsupporting

confidence: 72%

Priming to Mycobacterial Antigen In Vivo Using Antigen‐Pulsed Antigen Presenting Cells Generated In Vitro is Influenced by the Dose and Presence of IL‐4 in APC Cultures

Dillon

Hart

Abernethy³

et al. 1997

Scand J Immunol

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“…Professional APCs such as dendritic cells (DC), macrophages, and B lymphocytes are able to ingest, process, and present Ag in the context of MHC molecules (12,13). These cells have different immunologic functions.…”

Section: T Is Well Known That Traumatic Injury-induced Immunosup-mentioning

confidence: 99%

Trauma-Hemorrhage Induces Depressed Splenic Dendritic Cell Functions in Mice

Kawasaki

Hubbard

Choudhry

et al. 2006

The Journal of Immunology

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Although Kupffer cell, splenic, and peritoneal macrophage functions are markedly altered following trauma-hemorrhage (T-H), it remains unclear whether T-H also affects splenic dendritic cell (sDC) functions. We hypothesized that sDC functions will also be compromised following T-H. Male C3H/HeN (6- to 8-wk) mice were randomly assigned to sham operation or T-H. T-H was induced by midline laparotomy and ∼90 min of hemorrhagic shock (blood pressure 35 mmHg), followed by fluid resuscitation (four times the shed blood volume in the form of Ringer’s lactate). Two hours later, the mice were sacrificed; sDC were isolated; and the changes in their apoptosis, MHC class II expression, and ability to produce costimulatory cytokines and Ag presentation were measured. The results indicate that sDC Ag presentation capacity was significantly decreased and MHC class II expression was also significantly decreased following T-H. Moreover, LPS-induced IL-12 production and LPS- or IL-12-induced IFN-γ production following T-H were significantly decreased. Thus, the markedly decreased MHC class II expression and cytokine (IL-12, IFN-γ) production following T-H may be the cause for the depressed sDC Ag presentation under those conditions. This depression in Ag presentation could contribute to the host’s enhanced susceptibility to sepsis following T-H.

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“…4) exhibiting most if not all the features of professional antigen-presenting cells, the dendritic cells whose ontogeny have in fact recently been linked to the MPS [60][61][62][63][64]. These MO-derived dendritic cells (MO-DC) seem to be equivalent to DC derived from bone marrow progenitor cells [65] and have already been successfully used as cellular vaccines in clinical trials [66]; additional data is available from several animal trials.…”

Section: Monocyte-derived Cells To Present Antigenmentioning

confidence: 99%