Dendritic cells from different tissues induce production of different T cell cytokine profiles

Abstract

The precise role of antigen-presenting cells (APC) in regulating the balance of T-helper type 1 (Th1) and T-helper type 2 (Th2) cytokine production is unclear. Dendritic cells (DC), the most potent APC for activation of naive T cells, were found to regulate Th1 and Th2 cytokine profiles in a fashion dependent upon their tissue of origin. Spleen (systemic) DC induce mainly Th1 cytokines and Peyer's patch (mucosal) DC induce predominantly Th2 cytokines. These findings support the current concept that different tissues, each with its distinct microenvironment of cytokines, hormones, and cellular elements, are involved in the selection, promotion, and/or maintenance of different immune responses. With regard to DC, it is apparent that the tissue of DC origin determines the cytokine profiles produced by T cells and that DC from different tissues favor either cellular versus humoral immune responses by influencing T cell cytokine production.

Murine Peyer’s Patches Favor Development of an IL-10-Secreting, Regulatory T Cell Population

“…PP dendritic cells are distinct from those isolated from other secondary immune tissue and may contribute to the development of IL-10-secreting CD45RB low CD4 ϩ T cells. Dendritic cells residing in different tissues induce distinct immune responses from T cells (7,8,23). For instance, repetitive stimulation using immature human dendritic cells leads to differentiation of nonproliferative, IL-10-producing T cells with the ability to suppress proliferation of Th1 cells in vitro (16) Inhibiting IL-4, IL-10, and TGF-␤ in vitro did not appear to affect the functional response of PP T cells, suggesting that the effector function of these cells is established in vivo and is not readily modulated.…”

“…In the neighboring intrafollicular region of the PP, dendritic cells of a more mature phenotype than those found in the subepithelial dome lie in close association with T cells (6). Ag presentation by PP dendritic cells in vitro promotes splenic T cells to secrete IL-4, IL-6, and IL-10, in contrast to the proinflammatory cytokine, IFN-␥, induced by splenic dendritic cells (7)(8)(9). Furthermore, PP dendritic cells themselves secrete IL-10, an indication that, within the PP, T cells may be exposed to Ag in the presence of IL-10 (9).…”

Nature versus nurture in T cell cytokine production

“…The route of entry of antigens is an important extrinsic factor potentially affecting the available dose, form, and persistence of antigens, and the types of APC exposed [16,19]. Microenvironmental influences on T cell cytokine responses have been described and attributed to direct effects on T cells of local factors such as hormones [23] and/or indirect effects mediated by costimulatory molecules [22] and APC, particularly dendritic cells [20,24,25]. The latter effects have recently been extended to segregate dendritic cells, perhaps regardless of their tissue localization, into two lineages that may be committed to elicit different polarized cytokine responses from CD4 ϩ T cells [26,27].…”

Effect of fermented liquid diet prepared with Lactobacillus plantarum LQ80 on the immune response in weaning pigs

“…One of the reasons for the discrepancy is thought to be the difference of cell type to contact the bacteria. Antigen-presenting cells such as dendritic cells (DC) from different tissues, induce production of different T-cell cytokine profiles, while mucosal DC induce predominantly Th2 cytokines (Everson et al, 1996). Significance: *P , 0.05; **P , 0.01, ***P , 0.005.…”