Dendritic Cells, an Important New Cell Type in MHC-Linked Functions

Steinman, Ralph M.; Witmer, Margit D.; Nussenzweig, Michel C.; Chen, Lei L.; Kaplan, Gilla; Cohn, Zanvil A.

doi:10.1016/b978-0-12-708550-0.50027-7

Cited by 6 publications

(6 citation statements)

References 17 publications

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“…A modification of the original method of Steinman et al [19] was used. Briefly, spleen cell suspensions were prepared by digestion with a cocktail of 2.5 mg/ml collagenase (Worthington Biochemical Co., Freehold, NJ) and 0.1% DNase (Sigma Chemical Co.) in serum-free medium at 37°C for 2 x 40 min; low-density cells were isolated by centrifugation over a 60% Percoll gradient (p = 1.076) and the floating fraction collected.…”

Section: Dendritic Cell Preparationsmentioning

confidence: 99%

Interferon‐γ‐ and interleukin‐4‐producing T cells can be primed on dendritic cells in vivo and do not require the presence of B cells

Ronchese¹,

Hausmann²,

Gros

1994

Eur J Immunol

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The antigen-presenting cell (APC) requirements for the in vivo induction of Th1- and Th2-type responses were investigated using a severe combined immunodeficiency (SCID)mouse chimera model. SCID mice adoptively transferred with either T cells [SCID(T)] or T+B cells [SCID(T+B)] and immunized with antigen in adjuvant were able to generate antigen-specific T cells which could produce both interferon (IFN)-gamma and interleukin (IL)-4 upon in vitro restimulation. This suggests that B cell APC are not necessary for the priming of either IFN-gamma- or IL-4-producing T cells in vivo. The ability of different APC to activate Th2-dependent effector mechanisms was also investigated. SCID(T) and SCID(T + B) mice were infected with the nematode parasite Nippostrongylus brasiliensis and analyzed for the development of IL-5-dependent peripheral blood eosinophilia. Following infection both SCID(T) and SCID(T+B) mice generated similar numbers of peripheral blood eosinophils, suggesting that similar amounts of IL-5 had been produced. Therefore, B cell APC are also not required for the in vivo activation of Th2 cells to lymphokine production. To establish more precisely which APC prime T cells to produce IFN-gamma and IL-4, normal mice were immunized by injection of syngeneic splenic dendritic cells which had been pulsed with antigen in vitro. T cells from these immunized mice were able to produce good IFN-gamma and IL-4 responses upon in vitro restimulation with specific antigen; therefore, dendritic cells appear to be sufficient APC for the in vivo priming of both IFN-gamma- and IL-4-producing T cells.

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Section: Dendritic Cell Preparationsmentioning

confidence: 99%

Interferon‐γ‐ and interleukin‐4‐producing T cells can be primed on dendritic cells in vivo and do not require the presence of B cells

Ronchese¹,

Hausmann²,

Gros

1994

Eur J Immunol

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“…Ias-bearing cells were depleted by treatment with monoclonal antibodies 4-2.3 or 7-16.17. These antibodies have been previously described [9]. Macrophages were depleted by treatment with the Mac-1 monoclonal antibody, produced by the MU70 hybridoma [lo] obtained from American Type Culture Collection (Rockville, MD).…”

Section: Antiserum Depletionsmentioning

confidence: 99%

The defect in delayed‐type hypersensitivity of young adult SJL mice is due to a lack of functional antigen‐presenting cells

et al. 1985

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SJL mice exhibit a strain-specific age-dependent delay in the maturation of delayed-type hypersensitivity (DTH) responsiveness. They do not attain "adult" levels of DTH responsiveness until the 10th week of age, which is 4 to 6 weeks later than the other strains of mice tested. In this report we demonstrate that spleen cells, resident peritoneal cells and thioglycollate-elicited peritoneal exudate cells are all able to transfer DTH responsiveness from naive 12-week-old DTH responders to 6-week-old nonresponders. Transfer prior to immunization was more efficient at eliciting a response than transfer after immunization. As few as 5 X 10(4) cells from 12-week-old SJL mice can adoptively transfer responsiveness to unresponsive 6-week-old animals. The active cell was found to be adherent, radiation (2000 rds) resistant, I-A+, Thy-1- and Mac-1+. I-A compatibility between the adoptively transferred population and the nonresponder mice is required. These data suggest that young adult SJL mice lack a functional population of antigen-presenting cells specific for DTH and that the appearance of these cells is under maturational control.

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“…Both proliferative and cytotoxic components of the MLR are induced by DC (Steinman & Witmer 1978, Steinman et al 1979b. DC are at least two orders of magnitude more potent as allogeneic stimulators than either fractionated iymphoid cells or purified subpopulations of cells.…”

Section: A Allogeneic Mlrmentioning

confidence: 99%