Dendriplexes and their characterisation

Ramaswamy, Chandrasekaran; Sakthivel, Thiagarajan; Wilderspin, Andy; Florence, Alexander T.

doi:10.1016/s0378-5173(02)00670-1

Cited by 43 publications

(14 citation statements)

References 7 publications

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“…Iterative addition of lysine residues to the amine functionalized resin using glycine amino acid as a spacer resulted in a 3D structure with 64 surface primary amino groups surrounding a hyper-branched polyamide network. The multifunctional cationic nature of these lysine dendrimers leads to their interaction with DNA (Shah et al 2000;Ramaswamy et al 2003) and heparin (Al-Jamal et al 2003). We have also investigated interactions with albumin (Purohit et al 2003), liposomes (Purohit et al 2001), surfactants , as well as drug molecules such as benzylpenicillin (Al-Jamal et al 2004).…”

Section: Introductionmentioning

confidence: 99%

An intrinsically fluorescent dendrimer as a nanoprobe of cell transport

Al‐Jamal

Ruenraroengsak

Hartell

et al. 2006

Journal of Drug Targeting

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Dendrimers, spherical or quasi-spherical synthetic polymers in the nano-size range, have found useful applications as prospective carriers in drug and gene delivery. The investigation of dendrimer uptake by cells has been previously achieved by the incorporation of a fluorescent dye to the dendrimer either by chemical conjugation or by physical interaction. Here we describe the synthesis of two intrinsically fluorescent lysine based cationic dendrimers which lack a fluorophore, but which has sufficient fluorescence intensity to be detected at low concentrations. The nomenclature used to describe our compounds results in, for example the 6th generation dendrimer being notated as Gly-Lys(63) (NH2)(64); Gly denotes that the compound has a glycine in the core coupled to 63 lysine branching units (Lys(63)) and that the surface has 64 free amino groups (NH2)(64). The use of these dendrimers in probing transport avoids the need for fluorescent tagging with its attendant problems. The uptake of Gly-Lys(63) (NH2)(64) into Caco-2 cells was followed using confocal microscopy. Being cationic, it first adsorbs to the cell surface, enters the cytoplasm and reaches the nucleus within 35-45 min. Estimates of the diffusion coefficient of the dendrimer within the cell cytoplasm leads to a value of 6.27 ( +/- 0.49) x 10(-11) cm(2) s(-1), which is up to 1000 times lower than the diffusion coefficient of the dendrimer in water. Intrinsically fluorescent dendrimers of different size and charge are useful probes of transport in cells.

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Section: Introductionmentioning

confidence: 99%

An intrinsically fluorescent dendrimer as a nanoprobe of cell transport

Al‐Jamal

Ruenraroengsak

Hartell

et al. 2006

Journal of Drug Targeting

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“…Dendriplexes formed with low molecular weight dendrimer seemed to protect the DNA from DNase I enzyme even after 15 min of incubation at 37°C, as revealed by agarose gel electrophoresis, while naked DNA was completely digested in 2 min. The protection of the DNA component of these dendriplexes from nuclease Improved activity and stability of entrapped enzyme toward pH change, high temperature, organic solvent, and long-term storage, as compared to free GOD degradation was confirmed by DNase protection assays (Ramaswamy et al, 2003). The nature of interaction between DNA and dendrimer was demonstrated by Luo and Saltzman (2000).…”

Section: Dendrimer As Protein Stabilizermentioning

confidence: 87%

Carrier mediated protein and peptide stabilization

et al. 2010

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“…21 The results of our study show that these dendrimer nanoparticles are about 20 nm, and dendriplexes that formed with those plasmids that were well constructed could achieve 100-200 nm with neutral zeta potential. 22,23 The properties of the dendriplexes suggest that they can perform the transfection appropriately and affect tumor-cell proliferation and apoptosis. Nanomaterials are similar in scale to biologic molecules, and systems can yet be engineered to have various functions; therefore, nanotechnology is potentially useful for medical applications.…”

Section: Discussionmentioning

confidence: 99%