Dendrimers for drug delivery

Caminade, Anne‐Marie; Turrin, Cédric‐Olivier

doi:10.1039/c4tb00171k

Cited by 223 publications

(163 citation statements)

References 108 publications

Supporting

Mentioning

161

Contrasting

Unclassified

Order By: Relevance

“…Polymeric bottlebrushes, [1][2][3] dendrimers, 4,5 or comb 6 and arborescent 7 polymers are all examples of highly branched macromolecules (HBMs) that can be prepared in a well-defined manner and whose architecture endows them with a broad range of highly sought after properties for catalysis,4 drug delivery, 5 or enhanced lubrication at interfaces, 8 as contrast agents for imaging 9 or associative thickeners in paints. 10 If one focuses on anisotropic HBMs such as comb polymers or polymeric bottlebrushes, their characterization presents experimentalists with an additional challenge compared to linear chains since in theory, this characterization should be conducted along two perpendicular directions, one running axially along the main chain and the other running perpendicularly to the main axis along the side chain.…”

Section: Introductionmentioning

confidence: 99%

Probing Side Chain Dynamics of Branched Macromolecules by Pyrene Excimer Fluorescence

Farhangi

Duhamel

2015

Macromolecules

View full text Add to dashboard Cite

Four different pyrene-labeled polymers were prepared by radical copolymerization of n-butyl methacrylate (BMA) and 1-pyrenemethyl methacrylate (PyEG0MA), 1-pyrenemethoxyethyl methacrylate (PyEG1MA), 1-pyrenemethoxyethoxyethyl methacrylate (PyEG2MA), and 1-pyrenemethoxydiethoxyethyl methacrylate (PyEG3MA) to yield PyEG0-PBMA, PyEG1-PBMA, PyEG2-PBMA, and PyEG3-PBMA, respectively. The only structural difference between the polymers was the length of the oligo(ethylene glycol) spacer separating the pyrene label from the main chain. Steady-state and time-resolved fluorescence were applied to investigate how the length of the spacer affected the photophysical properties of the pyrene-labeled polymers. Excimer formation between an excited and a ground-state pyrene was enhanced by a longer spacer which increased the probability of encounter between two pyrene labels. This conclusion was supported through the analysis of the fluorescence decays of the polymers according to the Fluorescence Blob Model (FBM) which yielded the number (Nblob) of monomers constituting the volume in the polymer coil probed by an excited pyrene and the rate constant of excimer formation, kblob, inside a blob. Nblob increased more or less linearly with increasing spacer length reflecting a larger blob volume. kblob for PyEG0-PBMA was small due to steric hindrance while kblob took a larger but similar value within experimental error for all polymers labeled with pyrene derivatives having oligo(ethylene glycol) spacers. These experiments demonstrate that for a branched macromolecule, the volume probed by the tip of a side chain and its dynamics can be characterized quantitatively by monitoring pyrene excimer fluorescence. They are expected to provide important dynamic and structural information about the numerous highly branched macromolecules that are currently under intense scientific scrutiny.2

show abstract

Section: Introductionmentioning

confidence: 99%

Probing Side Chain Dynamics of Branched Macromolecules by Pyrene Excimer Fluorescence

Farhangi

Duhamel

2015

Macromolecules

View full text Add to dashboard Cite

show abstract

“…They allow packaging of larger molecules and are in particular useful to solubilize poorly water-soluble drugs. 4,5 Often the use of NPs in medicine is hampered by the fast binding of plasma proteins to their surfaces (opsonization), leading to quick clearance of the circulating NPs by the reticuloendothelial system, which is also called the mononuclear phagocytic system. [6][7][8] This may decrease the injected dose by as much as 50% within a few hours.…”

mentioning

confidence: 99%

The influence of surface charge on serum protein interaction and cellular uptake: studies with dendritic polyglycerols and dendritic polyglycerol-coated gold nanoparticles

Bewersdorff

Vonnemann

Kanik

et al. 2017

IJN

View full text Add to dashboard Cite

Nanoparticles (NPs) have gained huge interest in the medical field, in particular for drug delivery purposes. However, binding of proteins often leads to fast NP uptake and rapid clearance, thereby hampering medical applications. Thus, it is essential to determine and control the bio–nano interface. This study investigated the serum protein interactions of dendritic polyglycerols (dPGs), which are promising drug delivery candidates by means of two dimensional gel electrophoresis (2DE) in combination with mass spectrometry. In order to investigate the influence of surface charge, sulfated (sulfated dendritic polyglycerol [dPGS]) and non-sulfated (dPGOH) surfaces were applied, which were synthesized on a gold core allowing for easier separation from unbound biomolecules through centrifugation. Furthermore, two different sizes for dPGS were included. Although size had only a minor influence, considerable differences were detected in protein affinity for dPGS versus dPGOH surfaces, with dPGOH binding much less proteins. Cellular uptake into human CD14 + monocytes was analyzed by flow cytometry, and dPGOH was taken up to a much lower extent compared to dPGS. By using a pull-down approach, possible cellular interaction partners of serum pre-incubated dPGS-Au20 NPs from the membrane fraction of THP-1 cells could be identified such as for instance the transferrin receptor or an integrin. Clathrin-mediated endocytosis was further investigated using chlorpromazine as an inhibitor, which resulted in a 50% decrease of the cellular uptake of dPGS. This study could confirm the influence of surface charge on protein interactions and cellular uptake of dPGS. Furthermore, the approach allowed for the identification of possible uptake receptors and insights into the uptake mechanism.

show abstract

“…[1][2][3][4] These particular organizations result in unusual properties, as for example liquid-crystalline behavior at high concentrations, 5,6 anomalous intrinsic viscosity, 7,8 and a multivalent molecular surface. 9,10 As a consequence, dendrimers have been proposed for very useful applications, as for example, drug-delivery systems, 11,12 gene vectors, 13,14 catalysts 15,16 and organic light-emitting diodes. 17,18 DPs can be seen as wormlike macromolecular objects of cylindrical cross section.…”

Section: Introductionmentioning

confidence: 99%

“…Dendrimers are the more widely investigated polymers [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] and consist of perfectly branched molecules made of tree-like fragments (dendrons) attached to a central core. The internal organization and size of these welldefined, often monodisperse, molecules depends on the generation number, g, of dendrons.…”

Section: Introductionmentioning

confidence: 99%

Internal organization of macromonomers and dendronized polymers based on thiophene dendrons

et al. 2015

View full text Add to dashboard Cite

The internal organization of macromonomers (MGs) consisting of all-thiophene dendrons of generation g= 2 and 3 attached to a phenyl core, as well as of the dendronized polymers resulting from polymerization of these macromonomers (PG2 and PG3, respectively), has been investigated using theoretical methods. The conformational preferences of the MGs, determined using density functional theory calculations, are characterized by the relative orientation between dendrons and core.We find that the strain of the MGs increases with the generation number and is alleviated by small conformational re-arrangements of the peripheral thiophene rings.The conformations obtained for the MGs have subsequently been used to construct models for the dendronized polymers. Classical molecular dynamics simulations have evidenced that the interpenetration of dendrons belonging to different repeat units is very small for PG2. In contrast, the degree of interpenetration is found to be very high for PG3, which also shows a significant degree of backfolding (i.e. occurrence of peripheral methyl groups approaching the backbone). Consequently, PG2 behaves as a conventional linear flexible polymer bearing bulk pendant groups, whereas PG3 is better characterized as a semirigid homogenous cylinder. The two polymers are stabilized by - stacking interactions, even though these are significantly more abundant for PG3 than for PG2; the average number of interactions per repeat unit is 3.0 and 8.8 for PG2 and PG3, respectively. While in these interactions the thiophene rings can adopt either parallel (sandwich) or perpendicular (T-shaped) dispositions, the former scenario turns out to be the most abundant.3

show abstract

Dendrimers for drug delivery

Abstract: Schematized types of interactions of dendrimers with drugs or biologically active substances.

Cited by 223 publications

References 108 publications

Probing Side Chain Dynamics of Branched Macromolecules by Pyrene Excimer Fluorescence

Probing Side Chain Dynamics of Branched Macromolecules by Pyrene Excimer Fluorescence

The influence of surface charge on serum protein interaction and cellular uptake: studies with dendritic polyglycerols and dendritic polyglycerol-coated gold nanoparticles

Internal organization of macromonomers and dendronized polymers based on thiophene dendrons

Contact Info

Product

Resources

About