Dendrimer/DNA complexes encapsulated in a water soluble polymer and supported on fast degrading star poly(dl-lactide) for localized gene delivery

Fu, Huili; Cheng, Si‐Xue; Zhang, Xian‐Zheng; Zhuo, Ren‐Xi

doi:10.1016/j.jconrel.2007.08.031

Cited by 47 publications

(33 citation statements)

References 19 publications

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“…Ring-opening polymerization has been a method of choice, where the hydroxyl groups at positions C-3, C-7, and C-12 can be used as initiating points in the presence of DL-lactide, -caprolactone, or other cyclic ester monomers. [19][20][21][22][23][24][25] Zhuo et al exploited the hydroxyl groups of cholic acid (CA) to prepare a three arm star shaped polylactide with potential applications in drug delivery and as scaffolds for promoting cell adhesion and proliferation. However, high polydispersities and uneven chain lengths are shortcomings of ring-opening polymerization in the present context.…”

Section: Self-assembled Systemsmentioning

confidence: 99%

Polymers made of bile acids: from soft to hard biomaterials

Cunningham

Zhu

2016

Can. J. Chem.

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Bile acids are gaining increasing importance as building blocks in the development of novel polymeric materials. This is evidenced by the growing number of publications advocating the advantages of their incorporation in the design and construction of materials. Composed of a rigid steroid backbone, functional groups with potential towards diverse reactions, and a biocompatible framework, there are various ways in which these molecules can be utilized to afford biomaterials via distinct architectures. Soft materials utilize the intrinsic capacity of bile acids to self-assemble and have seen a range of applications, most notably in the field of drug delivery. On the other hand, there is also the possibility of including bile acids in the polymer backbone, which has been used in the preparation of elastomers. This review discusses a selection of materials that can be prepared using bile acids and the advantages afforded by these molecules. Focus will be on the development of soft and hard materials, where soft materials are described as being held by weak intermolecular interactions, whereas hard materials are mechanically stronger with bile acids covalently incorporated in the polymer network.Key words: bile acids, polymers, biomaterials, drug delivery, dental composites. Résumé :Les acides biliaires sont des synthons de plus en plus importants dans la mise au point de nouveaux matériaux à base de polymères, comme en témoigne le nombre croissant de publications préconisant leur incorporation dans la conception et la fabrication de ces matériaux. Composées d'un squelette stéroïdien rigide, de groupes fonctionnels au potentiel réactionnel diversifié, et d'une structure biocompatible, ces molécules, de par leurs différentes architectures, présentent diverses possibilités d'application dans le domaine des biomatériaux. La capacité intrinsèque des acides biliaires à s'autoassembler permet de produire des matériaux souples dont les applications sont variées, notamment dans le domaine de la libération des médica-ments. Par ailleurs, la possibilité d'intégrer des acides biliaires dans le squelette de polymères a aussi été explorée dans le cadre de la fabrication d'élastomères. Le présent article de synthèse porte sur une sélection de matériaux pouvant être préparés à partir d'acides biliaires et sur les avantages que comportent ces molécules. Une attention particulière est accordée à la mise au point de matériaux souples et de matériaux rigides. On y décrit les matériaux souples comme étant maintenus par des interactions intermoléculaires faibles, tandis que les matériaux rigides, dans lesquels les acides biliaires sont incorporés dans le réseau polymérique par des liaisons covalentes, sont mécaniquement plus solides. [Traduit par la Rédaction]

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Section: Self-assembled Systemsmentioning

confidence: 99%

Polymers made of bile acids: from soft to hard biomaterials

Cunningham

Zhu

2016

Can. J. Chem.

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“…Poly-,-[N-(2-hydroxyethyl)-L-aspartamide] (PHEA), a water-soluble polymer with good biocompatibility, has been proved to play an important role in protecting the PAMAM/DNA complexes during drying and improving the transfection efficiency of substrate-mediated transfections (Fu et al 2007). In the current study, to study the distribution of the PAMAM/DNA complexes embedded in PHEA in the microspheres, FITC-labelled PHEA was used to fabricate microspheres.…”

Section: Preparation and Characterization Of Microspheresmentioning

confidence: 99%

“…To overcome the disadvantages of the conventional methodologies for fabricating DNA-loaded microspheres, this study used a convenient and effective 'ultrasonic dispersion method' to fabricate vector/DNA complexes encapsulated microspheres using cholic acid functionalized star poly(DL-lactide), which degraded through surface erosion mechanism with a fast degradation rate (Fu et al 2007, Zou et al 2007a. During the preparation, vector/DNA complexes protected by a water-soluble polymer poly-,-[N-(2-hydroxyethyl)-L-aspartamide] (PHEA) were encapsulated in a polymer film.…”

Section: Introductionmentioning

confidence: 99%

Gene expression mediated by dendrimer/DNA complexes encapsulated in biodegradable polymer microspheres

Shao

et al. 2010

Journal of Microencapsulation

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A convenient and effective 'ultrasonic dispersion method' was used to fabricate vector/DNA complexes encapsulated microspheres. Polyamidoamine (PAMAM) dendrimer/DNA complexes protected by a water-soluble polymer, poly-alpha,beta-[N-(2-hydroxyethyl)-L-aspartamide] (PHEA), were encapsulated in a polymer film mainly composed of cholic acid functionalized star poly(DL-lactide), which degraded through surface erosion mechanism with a fast degradation rate. The PAMAM/DNA complexes encapsulated polymer film was then immersed in ethanol and ultrasonicated to afford the microspheres. The in vitro gene transfections showed PAMAM/DNA complexes protected by PHEA exhibited a much higher transfection activity compared with PAMAM/DNA complexes without the protection by PHEA. The expressions of pGL3-Luc in HEK293 cells could be effectively mediated by the polymer film and microspheres with the presence of PHEA. The ultrasonic dispersion method, which did not involve toxic organic solvents, could keep the bioactivity of DNA and offer good control over the size of microspheres.

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“…A series of star-shaped polymers were prepared with cholic acid as the core and DL-lactide, ε-caprolactone or other carbonates as branches through ring-opening polymerization by the group of Zhuo (Figure 8, A-D). These polymers can be useful in applications such as drug release [38][39][40][41][42] and gene delivery [43] , and can serve as scaffolds to promote cell attachment and growth [44,45] . When thermo-sensitive groups were attached onto position 24 of cholic acid, ringopening polymerization yielded thermo-sensitive drug carriers [46] .…”

Section: Bile Acids As the Core Of Star-shaped Polymersmentioning

confidence: 99%

Biomaterials made of bile acids

Zhang

Zhu

2009

Sci. China Ser. B-Chem.

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The use of natural compounds in the preparation of new materials can improve the biocompatibility of the materials and avoid any potential toxicity of the degradation products when used for biomedical applications. Bile acids are amphiphilic molecules biosynthesized in the liver. They are used to prepare various polymers and oligomers. These polymers made of bile acids are promising materials in both biomedical and pharmaceutical fields.

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Dendrimer/DNA complexes encapsulated in a water soluble polymer and supported on fast degrading star poly(dl-lactide) for localized gene delivery

Cited by 47 publications

References 19 publications

Polymers made of bile acids: from soft to hard biomaterials

Polymers made of bile acids: from soft to hard biomaterials

Gene expression mediated by dendrimer/DNA complexes encapsulated in biodegradable polymer microspheres

Biomaterials made of bile acids

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