Dencichine prevents ovariectomy-induced bone loss and inhibits osteoclastogenesis by inhibiting RANKL-associated NF-κB and MAPK signaling pathways

Cang, Dingwei; Zou, Guoyou; Yang, Chi; Shen, Xiaofei; Li, Feng; Wu, Ya; Ji, Biao

doi:10.1016/j.jphs.2021.04.004

Cited by 5 publications

(4 citation statements)

References 45 publications

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“…In this study, further evidence was offered by measuring the expression of osteoclast-related genes to make this point clearer. The RANKL gene can regulate the differentiation and function of osteoclasts by binding to RANK, which is located on the osteoclast membrane ( Cang et al, 2021 ). The activation of RANK promotes osteoclastic effects and increases bone resorption ( Liu et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Incorporation of Bone Morphogenetic Protein-2 and Osteoprotegerin in 3D-Printed Ti6Al4V Scaffolds Enhances Osseointegration Under Osteoporotic Conditions

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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Osteoporosis is an age-related metabolic disease that results in limited bone regeneration capacity and excessive osteoclast activity. After arthroplasty in patients with osteoporosis, poor interface osseointegration resulting from insufficient bone regeneration ability often leads to catastrophic complications such as prosthesis displacement and loosening and periprosthetic fractures. In this study, we prepared a thermosensitive hydrogel loaded with bone morphogenetic protein-2 (BMP-2) to promote osteogenesis and osteoprotegerin (OPG) to inhibit excessive osteoclast activity. To construct three-dimensional (3D)-printed composite scaffolds for implantation, a hydrogel loaded with drugs was injected into porous Ti6Al4V scaffolds. The 3D-printed composite scaffolds showed good biocompatibility and sustained release of BMP-2 and OPG for more than 20 days. In vitro experiments indicated that composite scaffolds promoted osteogenic differentiation and reduced the osteoclastic activation simultaneously. Remarkably, immunofluorescence staining, micro-CT, histological, and biomechanical tests demonstrated that the sustained release of both BMP-2 and OPG from composite scaffolds significantly improved bone ingrowth and osseointegration in osteoporotic defects. In conclusion, this study demonstrated that the BMP-2- and OPG-loaded 3D-printed composite scaffolds can potentially promote osseointegration for osteoporotic patients after joint replacement.

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Section: Resultsmentioning

confidence: 99%

Incorporation of Bone Morphogenetic Protein-2 and Osteoprotegerin in 3D-Printed Ti6Al4V Scaffolds Enhances Osseointegration Under Osteoporotic Conditions

Wang

et al. 2021

Front. Bioeng. Biotechnol.

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“…18 Recent studies indicated that dencichines are one of the main functional ingredients in P. notoginseng. It mainly has the functions of hemostasis, 19 increasing the number of platelets, 20,21 inhibiting the formation of osteoclasts, 22 as well as reducing the damage by diabetic nephropathy, 23 anti-inflammation, 24 and antioxidation. 25 addition to the Panax genus, 26 the same structure has also been identified in cycas L., 27 Acacia Mill., 28 and Lathyrus L. 29 Dencichine, also known as β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP), 29 is a nonprotein free amino acid and is soluble in water.…”

Section: ■ Introductionmentioning

confidence: 99%

“…notoginseng. It mainly has the functions of hemostasis, increasing the number of platelets, , inhibiting the formation of osteoclasts, as well as reducing the damage by diabetic nephropathy, anti-inflammation, and antioxidation . In addition to the Panax genus, the same structure has also been identified in cycas L., Acacia Mill., and Lathyrus L …”

Section: Introductionmentioning

confidence: 99%

Determination of Dencichine in Panax notoginseng in the Forest and Field Using High Performance Liquid Chromatography

Hua

et al. 2023

ACS Omega

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Dencichine is a nonprotein amino acid, an effective ingredient in Panax notoginseng with hemostatic and anti-inflammatory effects. There are few studies on the effects of regions and cultivation models on the accumulation of dencichine. In the current study, the content of dencichine in P. notoginseng collected from its global cultivation and trading center Yunnan, China, (>640 samples) was determined using an optimized high-performance liquid chromatography method coupled with a diode array detector but without derivatization. The recovery rate of this method was 80–110%, the relative standard deviation was <10%, and the limits of detection and quantification were 0.003% (w/w) and 0.01% (w/w), respectively. The content of dencichine in each part of P. notoginseng was as follows: rootlets (39.59%) > main roots (29.91%) > leaves (16.21%) > stems (14.29%). For leaves, P. notoginseng in the forest (5.52 ± 2.26 mg/g) was significantly higher than that in the field (3.93 ± 1.72 mg/g) but opposite for main roots. The origins and altitudes made different contributions to the accumulation of dencichine in P. notoginseng. This study provides an effective analytical method to determine dencichines in various parts of P. notoginseng from different origins and altitudes and supports quality control and product development of P. notoginseng.

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“…Dencichine (b-N-oxalyl-L-a, b-diaminopropionic acid, b-ODAP), isolated from the roots of panax notoginseng, has a high medicinal value [ 2 ]. Dencichine has been reported to show beneficial effects against numerous diseases, such as dispersing stasis and hemostasis, improving platelet number, relieving swelling and pain, kidney diseases, neuroprotection, lowering blood glucose [ 2 , 3 , 4 , 5 ] and rheumatic diseases [ 6 ]. Natural ingredients in plants are characterized by low concentration [ 7 ], the existence of multi-component mixtures, structural diversity and similarity, which pose great challenges to the separation and purification of natural ingredients [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation of Rosin-Based Composite Membranes and Study of Their Dencichine Adsorption Properties

Liu

et al. 2022

Polymers

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In this work, rosin-based composite membranes (RCMs) were developed as selective sorbents for the preparation of dencichine for the first time. The rosin-based polymer microspheres (RPMs) were synthesized using 4-ethylpyridine as a functional monomer and ethylene glycol maleic rosinate acrylate as a crosslinking. RCMs were prepared by spinning the RPMs onto the membranes by electrostatic spinning technology. The optimization of various parameters that affect RCMs was carried out, such as the ratio concentration and voltage intensity of electrospinning membrane. The RCMs were characterized by SEM, TGA and FT-IR. The performances of RCMs were assessed, which included adsorption isotherms, selective recognition and adsorption kinetics. The adsorption of dencichine on RCMs followed pseudo-second-order and adapted Langmuir–Freundlich isotherm model. As for the RCMs, the fast adsorption stage appeared within the first 45 min, and the experimental maximum adsorption capacity was 1.056 mg/g, which is much higher than the previous dencichine adsorbents reported in the literature. The initial decomposition temperature of RCMs is 297 °C, the tensile strength is 2.15 MPa and the elongation at break is 215.1%. The RCMs have good thermal stability and mechanical properties. These results indicated that RCMs are a tremendously promising adsorbent for enriching and purifying dencichine from the notoginseng extracts.

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Dencichine prevents ovariectomy-induced bone loss and inhibits osteoclastogenesis by inhibiting RANKL-associated NF-κB and MAPK signaling pathways

Cited by 5 publications

References 45 publications

Incorporation of Bone Morphogenetic Protein-2 and Osteoprotegerin in 3D-Printed Ti6Al4V Scaffolds Enhances Osseointegration Under Osteoporotic Conditions

Incorporation of Bone Morphogenetic Protein-2 and Osteoprotegerin in 3D-Printed Ti6Al4V Scaffolds Enhances Osseointegration Under Osteoporotic Conditions

Determination of Dencichine in Panax notoginseng in the Forest and Field Using High Performance Liquid Chromatography

Preparation of Rosin-Based Composite Membranes and Study of Their Dencichine Adsorption Properties

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