Denbinobin, a phenanthrene from dendrobium nobile, inhibits invasion and induces apoptosis in SNU-484 human gastric cancer cells

Abstract

Abstract. Dendrobium nobile is widely used as an analgesic, an antipyretic, and a tonic to nourish the stomach in traditional medicine. Mounting evidence suggests an antitumor activity of denbinobin, a major phenanthrene isolated from stems of Dendrobium nobile. The present study aimed to investigate the inhibitory effect of denbinobin on the invasive ability of human cancer cells. The cytotoxicity of denbonobin was examined in several human cancer cell lines including SK-Hep-1 hepatocarcinoma cells, SNU-484 gastric cancer cells, and HeLa cervix cancer cells. Because SNU-484 cells showed the lowest IC 50 value, we examined the effect of denbinobin on the invasive ability of SNU-484 cells. The present study revealed, for the first time, that denbinobin inhibits the invasive phenotype of SNU-484 human gastric cancer cells in a dose-dependent manner. Expressions of matrix metalloproteinase (MMP)-2 and MMP-9 were significantly decreased by denbinobin, suggesting that MMP-2/-9 may be responsible for the antiinvasive activity of denbinobin. We also provide evidence that denbinobin induces apoptosis through down-regulation of Bcl-2 and an up-regulation of Bax. Taken together, this study demonstrates that denbinobin inhibits invasion and induces apoptosis in highly invasive SNU-484 human gastric cancer cells. Given that gastric cancer has been estimated to be one of the most common causes of cancer-related death among Asians and the major cause of death from gastric cancer is the metastatic spread of the disease, our findings may provide useful information regarding the application of denbinobin as a chemopreventive agent that could prevent or alleviate metastatic gastric cancer.

Evaluation of Chemical Constituents and Important Mechanism of Pharmacological Biology inDendrobiumPlants

“…It significantly decreased the expressions of matrix metalloproteinase (MMP-2) and (MMP-9) and induced apoptosis through downregulation of Bcl-2 and upregulation of Bax in cancer cells. These findings may be used to provide reference for further studies on the pharmacological mechanisms from denbinobin and polysaccharides in D. nobile [ 50 , 51 , 84 ].…”

Moniliformediquinone as a potential therapeutic agent, inactivation of hepatic stellate cell and inhibition of liver fibrosis in vivo

“…Furthermore, CCl 4 -induced hepatic injury has been extensively used in animal models to evaluate the therapeutic potential of drugs. On the other hand, plants of the Dendrobium genus ( Orchidaceae ) are used in traditional herbal medicines in Asia, and studies have shown that they contain a wide variety of medicinal properties [ 11 – 13 ]. Moniliformediquinone (2,6-dimethoxy-1,4,5,8-phenanthradiquinone; MFD) (Fig.…”

Syringic Acid Extracted fromHerba dendrobiiPrevents Diabetic Cataract Pathogenesis by Inhibiting Aldose Reductase Activity

“…In ancient Chinese medical literature dating back over a thousand years, Dendrobium extracts were reported to improve vision [11]; however, there is still no firm understanding of the chemical components and mechanism of these extracts on vision. Recent research indicates that Dendrobium extracts may also have other bioactive properties, including the induction of apoptosis in human gastric cancer cells [12], the facilitation of neuroprotection against stress-induced apoptosis in PC12 cells [13], the inhibition of lipopolysaccharide- (LPS-) induced memory impairment in rats [14], and the LPS-induced nitric oxide production in macrophages [15]. It has also been suggested that Dendrobium extracts have strong antioxidant properties in vitro [16].…”