Denaturing gradient gel electrophoresis fingerprinting of soil bacteria in the vicinity of the Chinese Great Wall Station, King George Island, Antarctica

Pan, Qi; Feng, Wang; Zhang, Yang; Cai, Minghong; He, Jianfeng; Yang, Haizhen

doi:10.1016/s1001-0742(12)60229-0

Cited by 11 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2009, Pan et al . 2013). Thus, it appears that mature soil cannot be formed at Bellingshausen Station because of the constant impact of transport and human activity.…”

Section: Discussionmentioning

confidence: 99%

The impacts of deglaciation and human activity on the taxonomic structure of prokaryotic communities in Antarctic soils on King George Island

Pershina¹,

Иванова²,

Abakumov

et al. 2018

Antarctic Science

View full text Add to dashboard Cite

The soil microbiome was investigated at environmentally distinct locations on King George Island in the South Shetland Islands (Antarctic Peninsula) using 16 S rRNA gene pyrosequencing. The taxonomic composition of the soil prokaryotes (bacteria and archaea) was evaluated at three sites representing human-disturbed soils (Bellingshausen Station) and soils undergoing different stages of deglaciation (fresh and old moraines located near Ecology Glacier). The taxonomic analysis revealed 20 bacterial and archaeal phyla, among which Proteobacteria (29.6%), Actinobacteria (25.3%), Bacteroidetes (15.8%), Cyanobacteria (11.2%), Acidobacteria (4.9%) and Verrucomicrobia (4.5%) comprised most of the microbiome. In a beta-diversity analysis, the samples formed separate clusters. The Bellingshausen Station samples were characterized by an increased amount of Nostoc sp. and Janibacter sp. Although the deglaciation history had less of an effect on the soil microbiome, the early stages of deglaciation (Sample 1) had a higher proportion of bacteria belonging to the families Xanthomonadaceae, Sphingomonadaceae and Nocardioidaceae, whereas the older moraines (Sample 2) were enriched with Chthoniobacteriacae and N1423WL. Solirubrobacteriales, Gaiellaceae and Chitinophagaceae bacteria were present in both stages of deglaciation, characterized by genus-level differences. Taxonomic analysis of the abundant operational taxonomic units (OTUs) revealed both endemic (Marisediminicola antarctica, Hymenobacter glaciei) and cosmopolitan bacterial species in the microbiomes.

show abstract

“…2009, Pan et al . 2013). Thus, it appears that mature soil cannot be formed at Bellingshausen Station because of the constant impact of transport and human activity.…”

Section: Discussionmentioning

confidence: 99%

The impacts of deglaciation and human activity on the taxonomic structure of prokaryotic communities in Antarctic soils on King George Island

Pershina¹,

Иванова²,

Abakumov

et al. 2018

Antarctic Science

View full text Add to dashboard Cite

show abstract

“…Some studies have found that Antarctic soil bacterial communities differ according to the level of human impact in the sampling zone (e.g. [32,44,45]). Moreover, human activities may lead to direct or indirect consequences on animal behaviour and physiology, which could even have a negative impact on their reproduction and survival [35].…”

Section: Introductionmentioning

confidence: 99%

Seabird and pinniped shape soil bacterial communities of their settlements in Cape Shirreff, Antarctica

et al. 2019

View full text Add to dashboard Cite

Seabirds and pinnipeds play an important role in biogeochemical cycling by transferring nutrients from aquatic to terrestrial environments. Indeed, soils rich in animal depositions have generally high organic carbon, nitrogen and phosphorus contents. Several studies have assessed bacterial diversity in Antarctic soils influenced by marine animals; however most have been conducted in areas with significant human impact. Thus, we chose Cape Shirreff, Livingston Island, an Antarctic Specially Protected Area designated mainly to protect the diversity of marine vertebrate fauna, and selected sampling sites with different types of animals coexisting in a relatively small space, and where human presence and impact are negligible. Using 16S rRNA gene analyses through massive sequencing, we assessed the influence of animal concentrations, via their modification of edaphic characteristics, on soil bacterial diversity and composition. The nutrient composition of soils impacted by Antarctic fur seals and kelp gulls was more similar to that of control soils (i.e. soils without visible presence of plants or animals), which may be due to the more active behaviour of these marine animals compared to other species. Conversely, the soils from concentrations of southern elephant seals and penguins showed greater differences in soil nutrients compared to the control. In agreement with this, the bacterial communities of the soils associated with these animals were most different from those of the control soils, with the soils of penguin colonies also possessing the lowest bacterial diversity. However, all the soils influenced by the presence of marine animals were dominated by bacteria belonging to Gammaproteobacteria, particularly those of the genus Rhodanobacter. Therefore, we conclude that the modification of soil nutrient composition by marine vertebrates promotes specific groups of bacteria, which could play an important role in the recycling of nutrients in terrestrial Antarctic ecosystems.

show abstract

“…Hitherto, diverse soil bacterial communities have been observed in Antarctic terrestrial ecosystems using traditional isolation methods (Powell et al, 2006 ; Rinnan et al, 2009 ; Fan et al, 2013 ; Zdanowski et al, 2013 ) and traditional molecular methods (e.g., PCR-DGGE, cloning sequencing, real-time PCR, microarray) (Smith et al, 2006 , 2010 ; Shravage et al, 2007 ; Yergeau et al, 2007 , 2009 ; Niederberger et al, 2008 ; Yergeau and Kowalchuk, 2008 ; Aislabie et al, 2009 ; Chong et al, 2009 , 2010 ; Soo et al, 2009 ; Newsham et al, 2010 ; Ganzert et al, 2011 ; Ma et al, 2012 ; Stomeo et al, 2012 ; Pan et al, 2013 ; Teixeira et al, 2013 ). Recently, greater phylogenetic diversity of soil bacterial communities has been found in the Antarctic soils using Roche 454 sequencing (Teixeira et al, 2010 ; Kim et al, 2012 ; Lee et al, 2012 ; Roesch et al, 2012 ; Tiao et al, 2012 ; Yergeau et al, 2012 ), which is able to identify a great number of bacterial sequences and provide an in-depth analysis of soil bacterial diversity.…”

Section: Introductionmentioning

confidence: 99%

Diversity and structure of soil bacterial communities in the Fildes Region (maritime Antarctica) as revealed by 454 pyrosequencing

Wang

Zhang

et al. 2015

Front. Microbiol.

View full text Add to dashboard Cite

This study assessed the diversity and composition of bacterial communities in four different soils (human-, penguin-, seal-colony impacted soils and pristine soil) in the Fildes Region (King George Island, Antarctica) using 454 pyrosequencing with bacterial-specific primers targeting the 16S rRNA gene. Proteobacteria, Actinobacteria, Acidobacteria, and Verrucomicrobia were abundant phyla in almost all the soil samples. The four types of soils were significantly different in geochemical properties and bacterial community structure. Thermotogae, Cyanobacteria, Fibrobacteres, Deinococcus-Thermus, and Chlorobi obviously varied in their abundance among the 4 soil types. Considering all the samples together, members of the genera Gaiella, Chloracidobacterium, Nitrospira, Polaromonas, Gemmatimonas, Sphingomonas, and Chthoniobacter were found to predominate, whereas members of the genera Chamaesiphon, Herbaspirillum, Hirschia, Nevskia, Nitrosococcus, Rhodococcus, Rhodomicrobium, and Xanthomonas varied obviously in their abundance among the four soil types. Distance-based redundancy analysis revealed that pH (p < 0.01), phosphate phosphorus (p < 0.01), organic carbon (p < 0.05), and organic nitrogen (p < 0.05) were the most significant factors that correlated with the community distribution of soil bacteria. To our knowledge, this is the first study to explore the soil bacterial communities in human-, penguin-, and seal- colony impacted soils from ice-free areas in maritime Antarctica using high-throughput pyrosequencing.

show abstract

Denaturing gradient gel electrophoresis fingerprinting of soil bacteria in the vicinity of the Chinese Great Wall Station, King George Island, Antarctica

Cited by 11 publications

References 32 publications

The impacts of deglaciation and human activity on the taxonomic structure of prokaryotic communities in Antarctic soils on King George Island

The impacts of deglaciation and human activity on the taxonomic structure of prokaryotic communities in Antarctic soils on King George Island

Seabird and pinniped shape soil bacterial communities of their settlements in Cape Shirreff, Antarctica

Diversity and structure of soil bacterial communities in the Fildes Region (maritime Antarctica) as revealed by 454 pyrosequencing

Contact Info

Product

Resources

About