Denatured acellular dermal matrix seeded with bone marrow mesenchymal stem cells for wound healing in mice

Qi, Yu; Dong, Zheng-xue; Chu, Hongzhen; Zhao, Qi; Wang, Xiao; Yang, Jiao; Gong, Hongmin; Pan, Yi; Jiang, Duyin

doi:10.1016/j.burns.2019.04.017

Cited by 17 publications

(11 citation statements)

References 40 publications

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“…To date, autologous skin grafting following early excision of necrotic tissue has been widely used as the best approach for skin repair and reconstruction [8]. However, autologous skin is usually insufficient for patients with large-area burns due to the lack of donor skin, and allogenic or heterogeneous skin would lead to a strong immune response [9]. With the development of tissue engineering, tissue-engineered skin grafting is considered to be an effective alternative treatment for severe burn injuries with large-area skin defects [10].…”

Section: Introductionmentioning

confidence: 99%

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Zhang

Wang

Wei

et al. 2021

Gels

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Tissue-engineered skin grafts have long been considered to be the most effective treatment for large skin defects. Especially with the advent of 3D printing technology, the manufacture of artificial skin scaffold with complex shape and structure is becoming more convenient. However, the matrix material used as the bio-ink for 3D printing artificial skin is still a challenge. To address this issue, sodium alginate (SA)/carboxymethyl cellulose (CMC-Na) blend hydrogel was proposed to be the bio-ink for artificial skin fabrication, and SA/CMC-Na (SC) composite hydrogels at different compositions were investigated in terms of morphology, thermal properties, mechanical properties, and biological properties, so as to screen out the optimal composition ratio of SC for 3D printing artificial skin. Moreover, the designed SC composite hydrogel skin membranes were used for rabbit wound defeat repairing to evaluate the repair effect. Results show that SC4:1 blend hydrogel possesses the best mechanical properties, good moisturizing ability, proper degradation rate, and good biocompatibility, which is most suitable for 3D printing artificial skin. This research provides a process guidance for the design and fabrication of SA/CMC-Na composite artificial skin.

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Section: Introductionmentioning

confidence: 99%

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Zhang

Wang

Wei

et al. 2021

Gels

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“…Firstly, ACMs were incubated with ASCs to assess the interaction between the acellular scaffold and mesenchymal stem cells, representing one of the cellular elements involved in regeneration and repair pathways [27]. According to the literature, the stimulation and differentiation of ASCs increase wound repair and regeneration ability by intensifying collagen secretion and deposition [9,14]. Recent studies have demonstrated a link between adipocytes and wound healing, given that adipocytes also act as key regulators of skin health [28].…”

Section: Discussionmentioning

confidence: 99%

“…ACMs can be used to treat difficult-to-heal wounds, deep burn closure, and as a scaffold for organ parts and tissue reconstruction due to the capacity to support host tissue cell colonisation and promote their differentiation [7,9,10]. ACMs healing ability is regarding their capacity to induce wound repair by amplifying the collagen secretion and deposition and adjusting the healing reparative phases [11,12].…”

Section: Introductionmentioning

confidence: 99%

Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices

Sierra

Busato

Zingaretti

et al. 2022

Tissue Eng Regen Med

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Background: Breast reconstruction after mastectomy using silicone implants is a surgical procedure that occasionally leads to capsular contracture formation. This phenomenon constitutes an important and persistent cause of morbidity, and no successful therapies are available to date. Recently, the use of acellular membranes as a protective material for silicone prostheses has been gaining attention due to their ability to prevent this adverse outcome. For this reason, the evaluation of the tissue-material integration and the induced biostimulation by acellular membranes results crucial. Evaluation of in vivo tissue integration and biostimulation induced by three different natural acellular collagen membranes. Methods: Scanning electron microscopy was performed to analyse the membrane porosity and cells-biomaterial interaction in vitro, both in dry and wet conditions. Adipose-derived stem cells were cultured in the presence of membranes, and the colonisation capacity and differentiation potential of cells were assessed. In vivo tests and ex vivo analyses have been performed to evaluate dermal integration, absorption degree and biostimulation induced by the evaluated membrane. Results: Analysis performed in vitro on the three different acellular dermal matrices evidenced that porosity and the morphological structure of membranes influence the liquid swelling ratio, affecting the cell mobility and the colonisation capacity. Moreover, the evaluated membranes influenced in different manner the adipose derived stem cells differentiation and their survival. In vivo investigation indicated that the absorption degree and the fluid accumulation surrounding the implant were membrane-dependent. Finally, ex vivo analysis confirmed the membrane-dependent behavior revealing different degree of tissue integration and biostimulation, such as adipogenic stimulation. Conclusion: The physico-chemical characteristics of the membranes play a key role in the biostimulation of the cellular environment inducing the development of well-organized adipose tissue.

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“…One reason we believe ADG performed so well in our experiment was that the revascularisation process for ADG was inherently different from other dermal substitutes. 18,[21][22][23] Since ADGs contained intact blood vessels, revascularisation through an ADG required two separate inosculation events (one at the wound bed-ADG interface and the other at the ADG-STSG interface) but no new vessel growth. Acellular substitutes, conversely, would require the regrowth of blood vessels through the entirety of the substitute while the graft survived on imbibition from the wound bed or inosculation from the lateral wound edges.…”

Section: Discussionmentioning

confidence: 99%

Impact of dermal matrix thickness on split‐thickness skin graft survival and wound contraction in a single‐stage procedure

Bohan

Cooper

Fletcher

et al. 2021

International Wound Journal

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Optimal treatment of full-thickness skin injuries requires dermal and epidermal replacement. To spare donor dermis, dermal substitutes can be used ahead of split-thickness skin graft (STSG) application. However, this two-stage procedure requires an additional general anaesthetic, often prolongs hospitalisation, and increases outpatient services. Although a few case series have described successful single-stage reconstructions, with application of both STSG and dermal substitute at

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Denatured acellular dermal matrix seeded with bone marrow mesenchymal stem cells for wound healing in mice

Cited by 17 publications

References 40 publications

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Design and Fabrication of Sodium Alginate/Carboxymethyl Cellulose Sodium Blend Hydrogel for Artificial Skin

Tissue-Material Integration and Biostimulation Study of Collagen Acellular Matrices

Impact of dermal matrix thickness on split‐thickness skin graft survival and wound contraction in a single‐stage procedure

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