Denaturation and reactivity of invertase in frozen solutions

Tong, Min-Min; Pincock, Richard E.

doi:10.1021/bi00831a021

Cited by 14 publications

(5 citation statements)

References 16 publications

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“…Rank 1 corresponds to the lowest entropy and so on. For the purpose of these examples, consider a specific rank ordering: (12) Note that rank 0 is used for boundary conditions, which are not discussed here, but are detailed in previous work. 32 First consider a triplet of states, aāc, that is spanned by a Hbond.…”

Section: Appendix Amentioning

confidence: 99%

“…The effect of a protein denaturing upon heating or cooling has been well established more than 30 years ago, 12 where cold denaturation is more pronounced under mixed solvent conditions 13 and/or high pressures. 14, 15 Privalov and Gill 16 proposed the explanation that hydration (structured water) is the mechanism responsible for cold denaturation.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Understanding the α‐helix to coil transition in polypeptides using network rigidity: Predicting heat and cold denaturation in mixed solvent conditions

Jacobs¹,

Wood²

2004

Biopolymers

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Thermodynamic stability in polypeptides is described using a novel Distance Constraint Model (DCM). Here, microscopic interactions are represented as constraints. A topological arrangement of constraints define a mechanical framework. Each constraint in the framework is associated with an enthalpic and entropic contribution. All accessible topological arrangements of distance constraints form an ensemble of mechanical frameworks, each representing a microstate of the polypeptide. A partition function is calculated exactly using a transfer matrix approach, where in many respects the DCM is similar to the Lifson-Roig model. The crucial difference is that the effect of network rigidity is explicitly calculated for each mechanical framework in the ensemble. Network rigidity is a mechanical interaction that provides a mechanism for long-range molecular cooperativity and enables a proper treatment of the nonadditivity of a microscopic free energy decomposition. Accounting for (1) helix ↔ coil conformation changes along the backbone similar to the Lifson-Roig model, (2) i to i + 4 hydrogen-bond formation ↔ breaking similar to the Zimm-Bragg model, and (3) structured ↔ unstructured solvent interaction (hydration effects), a six-parameter DCM describes normal and inverted helix-coil transitions in polypeptides. Under suitable mixed solvent conditions heat and cold denaturation is predicted. Model parameters are fitted to experimental data showing different degrees of cold denaturation in monomeric polypeptides in aqueous hexafluoroisopropanol (HFIP) solution at various HFIP concentrations. By assuming a linear HFIP concentration dependence (up to 6% by mole fraction) on model parameters, all essential experimentally observed features are captured.

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Section: Appendix Amentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Understanding the α‐helix to coil transition in polypeptides using network rigidity: Predicting heat and cold denaturation in mixed solvent conditions

Jacobs¹,

Wood²

2004

Biopolymers

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show abstract

“…Even in the case Fennema, 1966. Thus, small increases were observed in fro zen systems relative to otherwise identical supercooled systems (e.g., at -4°C) in the rate of hydrolysis of sucrose by invertase (Tong and Pincock, 1969). d 0 = undetectable.…”

Section: Secondary Freeze-dehydration Injurymentioning

confidence: 89%

Molecular Basis of Freezing Injury and Tolerance

Levitt

1980

Chilling, Freezing, and High Temperature Stresses

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“…than temperature lowering alone and the effects of freezing are therefore not always consistent with the pattern in Figure 1. Although rare, freezing can, for example, actually cause an increase in the rate constant for some enzyme-catalyzed reactions (19,20).…”

Section: Factors Influencing Enzyme Activity At Low Temperaturesmentioning

confidence: 99%

Behavior of Proteins at Low Temperatures

Fennema

1982

ACS Symposium Series

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Denaturation and reactivity of invertase in frozen solutions

Cited by 14 publications

References 16 publications

Understanding the α‐helix to coil transition in polypeptides using network rigidity: Predicting heat and cold denaturation in mixed solvent conditions

Understanding the α‐helix to coil transition in polypeptides using network rigidity: Predicting heat and cold denaturation in mixed solvent conditions

Molecular Basis of Freezing Injury and Tolerance

Behavior of Proteins at Low Temperatures

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