den V gene of bacteriophage T4 determines a DNA glycosylase specific for pyrimidine dimers in DNA

Abstract

to protein at 0°C. Each reaction contained '4C-labeled ColEl DNA (approximately 100 ng, 5,000 cpm) irradiated with 200 J/m2 at 254 nm. Nicking of DNA was assayed with superhelical 14C-790

DNA base repair – recognition and initiation of catalysis

“…bacteriophage T4) and Phycodnaviridae (e.g. chlorella virus) families, as well as the bacteria Micrococcus luteus encode a DNA glycosylase with associated AP lyase activity specific for CPDs (Haseltine et al, 1980;Radany & Friedberg, 1980;Seawell et al, 1980;McMillan et al, 1981;Nakabeppu & Sekiguchi, 1981;McCullough et al, 1998). Sequence homologs of the T4 pyrimidine dimer glycosylase (T4-Pdg) are also present in distantly related bacterial genomes such as Bordetella, Brucella, Haemophilus, Pasteurella and Prochlorococus.…”

Simian virus 40 as a probe for studying inducible repair functions in mammalian cells

“…We carried out similar experiments after UV irradiation of host cells during SV40 replication; 24 hr after SV40 infection, cells were UV irradiated with a germicidal lamp at 125J/m2. We measured that this UV dose induces about 5.5 pyrimidine dimers per SV40 DNA molecule (3.5 x lo6 d) by using the Tq endonuclease V assay, as described by P. C. Seawell et a1 [16].…”

Inducible error-prone repair in B. subtilis. Final report, September 1, 1979-June 30, 1981

“…These alternate pathways of excision repair have been associated with error-prone components (21,22). Also, in Micrococcus luteus and bacteriophage T4-infected E. coli, the.uv excision repair system has been shown to involve glycosylase (23,24). These glycosylases cause the rupture of the glycosylic bond between one of the pyrimidines of the dimer and the deoxyribose sugar leaving an apyrimidinic site (23,24,25).…”

“…Also, in Micrococcus luteus and bacteriophage T4-infected E. coli, the.uv excision repair system has been shown to involve glycosylase (23,24). These glycosylases cause the rupture of the glycosylic bond between one of the pyrimidines of the dimer and the deoxyribose sugar leaving an apyrimidinic site (23,24,25). The apyrimidinic site is then cleaved by an AP {apurinic or apyrimidinic) endonuclease (23,24,26).…”

“…These glycosylases cause the rupture of the glycosylic bond between one of the pyrimidines of the dimer and the deoxyribose sugar leaving an apyrimidinic site (23,24,25). The apyrimidinic site is then cleaved by an AP {apurinic or apyrimidinic) endonuclease (23,24,26). This type of excision repair is analogous to the 'base excision repair' systems which remove altered bases in the DNA of different organisms (27,28).…”