Demonstration of the role of cytophilic antibody in resistance to malaria parasites (Plasmodium berghei) in rats

Green, T J; Kreier, Julius P.

doi:10.1128/iai.19.1.138-145.1978

Cited by 31 publications

(13 citation statements)

References 7 publications

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“…Absence of schizont ingestion was recorded when we used either 'immune monocytes' (Khusmith et al 1982) or in vitro armed monocytes. This is in agreement with the studies conducted by Kreier and co-workers using rat macrophages and P. berghei infected red cells (Chow & Kreier 1972, Green & Kreier 1978, Hamburger & Kreier 1976, Brooks & Kreier 1978, and contrasts with studies performed using mouse peritoneal macrophages and erythrocytes infected with P . berghei (Hunter et al 1979), P .…”

Section: Discussionsupporting

confidence: 92%

“…Furthermore, immunoglobulins with antimalarial specificity were able to adhere to the monocytes and could be eluted from them. These cytophilic antibodies are probably attached via their Fc region to receptors on the surfaces ofmonocytes (Green & Kreier 1978, Berken & Benacerraf 1966.…”

Section: Discussionmentioning

confidence: 99%

“…Specific antibodies also co-operate with cells in clearance of malaria parasites. An enhancing effect of immune serum on phagocytosis by macrophages was demonstrated in uitro in simian and rodent malaria, and appears to be due to the presence of at least two different antibodies in the immune serum: opsonin acting on the free parasites (Chow & Kreier 1972, Green & Kreier 1978, Hamburger & Kreier 1976, Brooks & Kreier 1978 or parasitized erythrocytes (Cohen & Butcher 1970, Hunter, Winkelstein & Simpson 1979, Tosta & Wedderburn 1980) and a macrophage-cytophilic antibody (Tosta 1976, Green & Kreier 1978.…”

Section: Skhusmith and Pdrui1hementioning

confidence: 99%

“…The monocyte monolayers were prepared by the method described above and were then held at 4°C until used. The absorption of cytophilic antibody component out of hyperimmune antiserum using normal macrophages and the elution from them were carried out by the method described by Green & Kreier (1978).…”

Section: Elution Of Cytophilic Antibodymentioning

confidence: 99%

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Antibody‐dependent ingestion of P. falciparum merozoites by human blood monocytes

Khusmith

Druilhe

1983

Parasite Immunology

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The binding of malarial antibodies to peripheral blood monocytes and the ability of these armed monocytes to attach to and ingest P. falciparum merozoites and schizont infected erythrocytes was evaluated by an in vitro assay. Monocytes from normal unsensitized subjects were preincubated with sera from individuals with various states of immunity to malaria and sera from normal controls. A marked difference in the level of merozoite phagocytosis was observed depending on immune status of the individuals whose sera were tested, but not on the antibody levels measured by fluorescence or precipitation tests. By protein-A-sepharose fractionation of these sera it appeared that the merozoite phagocytosis was mediated by immunoglobulin of the IgG class. Immunoglobulins eluted from these preincubated monocytes were able to bind to the parasites as detected by indirect fluorescent test. Similar assays performed with different strains and antibodies from various geographical areas indicated that the merozoite recognition and ingestion was not strain specific. The monocyte-immunoglobulin co-operation was effective in the phagocytosis of merozoites but not of schizont infected erythrocytes or normal erythrocytes. However, some degree of adhesion to the schizonts was recorded. These data seem to indicate that human blood monocytes can be specifically armed in vitro by cytophilic IgG with antimalarial specificity, and that such an effect is able to enhance markedly the clearance of free parasites but not of intact schizonts.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Skhusmith and Pdrui1hementioning

confidence: 99%

Section: Elution Of Cytophilic Antibodymentioning

confidence: 99%

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Antibody‐dependent ingestion of P. falciparum merozoites by human blood monocytes

Khusmith

Druilhe

1983

Parasite Immunology

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“…Several studies have demonstrated the role of serum antibodies in P. berghei immunity (12,15,20,23,24,28,29,37,40,42). The passive transfer of immunity by lymphoid cells of rats recovered from P. berghei infection (17,37) was demonstrated to be due to B-type-antibody-producing lymphocytes (27).…”

Section: Discussionmentioning

confidence: 99%

Correlation between genetic regulation of antibody responsiveness and protective immunity induced by Plasmodium berghei vaccination

Heumann¹,

Stiffel²,

Monjour³

et al. 1979

Infect Immun

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High (H) and low (L) antibody responder lines of mice were produced by two independent bidirectional selective breedings for quantitative antibody responsiveness to heterologous erythrocytes (selection I and selection II). In both selections the antibody response to P. berghei antigens was 8- to 10-fold higher in H than in L lines. The character "high response" presents an incomplete dominance o- 18% in selection I and 67% in selection II. In selection II the variance analysis indicates that at least three independent loci intervene in the regulation of responsiveness to P. berghei antigens. The innate resistance and the protective efficacy of vaccination against P. berghei infection induced by parasitized erythrocytes was measured in H and L lines and in the interline hybrids F1, BcH, and BcL of selections I and II. No very significant difference was observed in the innate resistance to P. berghei infection between H and L mice of both selections. Vaccination induced a very efficient protection in the two H lines (94 and 95% survival), whereas only a weak protection was induced in the two L lines (16 and 31% survival); the degree of protection is intermediate in interline hybrids F1, BcH, and BcL. In both selections a good linear correlation was demonstrated between the level of vaccination-induced antibody and the degree of resistance measured as percentage of survival. The present results indicate that the vaccination-induced P. berghei immunity is essentially due to the antibody response, whereas the bactericidal activity of macrophages and the cell-mediated immunity do not play a determinant role.

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Roles of Surface Antigens on Malaria-Infected Red Blood Cells in Evasion of Immunity

Howard

Barnwell

1984

Immunobiology of Parasites and Parasitic Infections

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Demonstration of the role of cytophilic antibody in resistance to malaria parasites (Plasmodium berghei) in rats

Cited by 31 publications

References 7 publications

Antibody‐dependent ingestion of P. falciparum merozoites by human blood monocytes

Antibody‐dependent ingestion of P. falciparum merozoites by human blood monocytes

Correlation between genetic regulation of antibody responsiveness and protective immunity induced by Plasmodium berghei vaccination

Roles of Surface Antigens on Malaria-Infected Red Blood Cells in Evasion of Immunity

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