Demonstration of the cellular viability and safety of<i>Enterococcus faecium</i>CRL 183 in long-term experiments

Cano, Veridiana P. S.; Valentini, Sandro Roberto; Sivieri, Kátia; Rossi, Elizeu Antônio

doi:10.1051/lait:2007002

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…Cultures were harvested during the exponential growth, after, they were centrifuged (4000 × g, 10 min, 4°C) and washed with sterile peptone water. The L. acidophilus CRL1014 cells were kept at the concentration of 10 8 CFU/mL in sterile peptone water until use [17]. …”

Section: Methodsmentioning

confidence: 99%

Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME®reactor

et al. 2013

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BackgroundHow to maintain “gut health” is a goal for scientists throughout the world. Therefore, microbiota management models for testing probiotics, prebiotics, and synbiotics have been developed.MethodsThe SHIME® model was used to study the effect of Lactobacillus acidophilus 1014 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2-wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota was monitored. The microbiota was then subjected to a 4-wk treatment period by adding 5 mL of sterile peptone water with L. acidophilus CRL1014 at the concentration of 108 CFU/mL to vessel one (the stomach compartment). Plate counts, Denaturing Gradient Gel Electrophoresis (DGGE), short-chain fatty acid (SCFA) and ammonium analyses were carried out for monitoring of the microbial community from the colon compartments.ResultsA significant increase (p < 0.01) in the Lactobacillus spp. and Bifidobacterium spp. populations was observed during the treatment period. The DGGE obtained showed changes in the lactobacilli community from the colon compartments of the SHIME® reactor. The (SCFA) concentration increased (p < 0.01) during the treatment period, due mainly to significant increased levels of acetic, butyric, and propionic acids. However, ammonium concentrations decreased during the same period (p < 0.01).ConclusionsThis study showed the beneficial influence of L. acidophilus CRL 1014 on microbial metabolism and lactobacilli community composition for improving human health.

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Section: Methodsmentioning

confidence: 99%

Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME®reactor

et al. 2013

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Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183

Sivieri¹,

Bianchi²,

Tallarico³

et al. 2011

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Background: Enterococci are used in a large number of dairy products, such as starter cultures in food supplements and in foods considered functional. In vitro gut fermentation models present an unmatched opportunity of performing studies frequently allenged in humans and animals owing to ethical concerns. A dynamic model of the human intestinal microbial ecosystem (SHIME) was designed to better simulate conditions intestinal microbiota.Methods: The SHIME model was used to study the effect of Enterococuus faecium CRL 183 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2 wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota were monitored. The microbiota were then subjected to a 4-wk treatment period by adding 108 CFU/mL of the Enterococcus faecium CRL 183 to vessel one (the stomach compartment).Results: The addition resulted into an overall increase of bacterial marker populations (Enterobacteriaceae, Lactobacillus spp., Bifidobacterium spp. and Clostridium spp.), with a significant increase of the Lactobacillus sp. and Bifidobacterium sp populations. The short-chain fatty acid (SCFA) concentration increased during the supplementation period; this was due mainly to a significant increase in the levels of acetic, butyric and propionic acids. Ammonium concentrations increased during the supplementation period.Conclusions: Results showed that the major effect of E. faecium CRL 183 was found in the ascendant and transverse colon. Key words: Gut microbiota, Enterococcus, Gastrointestinal resource management, Simulator of Human Intestinal Microbial Ecosystem (SHIME)

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Demonstration of the cellular viability and safety ofEnterococcus faeciumCRL 183 in long-term experiments

Cited by 2 publications

References 34 publications

Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME®reactor

Lactobacillus acidophilus CRL 1014 improved “gut health” in the SHIME®reactor

Fermentation by gut microbiota cultured in a simulator of the human intestinal microbial ecosystem is improved by probiotic Enterococcus faecium CRL 183

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