Demonstration of the Agent of Legionnaires’ Disease in Tissue

Chandler, Francis W.; Hicklin, Martin D.; Blackmon, John A.

doi:10.1056/nejm197712012972206

Cited by 143 publications

(46 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Our results for sensitivity (44%) are slightly lower than those reported by Theaker et al (51) (six of nine positives detected) and higher that those noted by Koide et al (29) (two of eight positives detected). In comparison, for the current study examination of WS-stained slides demonstrated a sensitivity of 63%, which is similar to the results obtained in an earlier study using the Dieterle silver impregnation method (4).…”

Section: Discussionsupporting

confidence: 91%

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

et al. 2001

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 91%

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

et al. 2001

View full text Add to dashboard Cite

show abstract

“…First, in lung specimens from patients with Legionnaires' disease, clusters of bacteria have been observed in alveolar macrophages (19,20). Similar findings have been reported in the lungs ofexperimentally infected guinea pigs (21).…”

Section: Introductionmentioning

confidence: 99%

Legionnaires' Disease Bacterium (Legionella pneumophila) Multiplies Intracellularly in Human Monocytes

Horwitz¹,

Silverstein²

1980

J. Clin. Invest.

626

524

View full text Add to dashboard Cite

A B S T R A C T We have studied the interaction between virulent egg yolk-grown Legionella pneumophila Philadelphia 1 and human blood monocytes in vitro. The leukocytes were cultured in antibiotic-free tissue culture medium supplemented with 15% autologous human serum.L. pneumophila multiplied several logs, as measured by colony-forming units, when incubated with monocytes or mononuclear cells; the mid-log phase doubling time was 2 h. The level to which L. pneumophila multiplied was proportional to the number of mononuclear cells in the culture. L. pneumophila multiplied only in the adherent fraction of the mononuclear cell population indicating that monocytes but not lymphocytes support growth of the bacteria. Peak growth ofL. pneumophila was correlated with destruction of the monocyte monolayer. By fluorescence microscopy using fluorescein conjugated rabbit anti-L. pneumophila antiserum, the number of monocytes containing L. pneumophila increased in parallel with bacterial growth in the culture. At the peak of infection, monocytes were packed full with organisms. By electron microscopy, L. pneumophila in such monocytes were found in membrane-bound cytoplasmic vacuoles studded with structures resembling host cell ribosomes.

show abstract

“…Pathogenesis of the bacterium within mammalian hosts and its ability to grow within amoebae are closely linked. In human pneumonia, the microorganism grows in alveolar macrophages, cells that are phagocytic and motile like amoebae (8,23). Furthermore, L. pneumophila mutants defective for growth in macrophages also show defective growth in amoebae (18,43).…”

mentioning

confidence: 99%

Intracellular Growth ofLegionella pneumophilainDictyostelium discoideum, a System for Genetic Analysis of Host-Pathogen Interactions

et al. 2000

View full text Add to dashboard Cite

Conditions were established in which Legionella pneumophila, an intracellular bacterial pathogen, could replicate within the unicellular organism Dictyostelium discoideum. By several criteria, L. pneumophila grew by the same mechanism within D. discoideum as it does in amoebae and macrophages. Bacteria grew within membrane-bound vesicles associated with rough endoplasmic reticulum, and L. pneumophila dot/icm mutants, blocked for growth in macrophages and amoebae, also did not grow in D. discoideum. Internalized L. pneumophila avoided degradation by D. discoideum and showed evidence of reduced fusion with endocytic compartments. The ability of L. pneumophila to grow within D. discoideum depended on the growth state of the cells. D. discoideum grown as adherent monolayers was susceptible to L. pneumophila infection and to contact-dependent cytotoxicity during high-multiplicity infections, whereas D. discoideum grown in suspension was relatively resistant to cytotoxicity and did not support intracellular growth. Some known D. discoideum mutants were examined for their effect on growth of L. pneumophila. The coronin mutant and the myoA/B double myosin I mutant were more permissive than wild-type strains for intracellular growth. Growth of L. pneumophila in a G ␤ mutant was slightly reduced compared to the parent strain. This work demonstrates the usefulness of the L. pneumophila-D. discoideum system for genetic analysis of host-pathogen interactions.

show abstract

Demonstration of the Agent of Legionnaires’ Disease in Tissue

Cited by 143 publications

References 6 publications

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

Direct Detection of Legionella Species from Bronchoalveolar Lavage and Open Lung Biopsy Specimens: Comparison of LightCycler PCR, In Situ Hybridization, Direct Fluorescence Antigen Detection, and Culture

Legionnaires' Disease Bacterium (Legionella pneumophila) Multiplies Intracellularly in Human Monocytes

Intracellular Growth ofLegionella pneumophilainDictyostelium discoideum, a System for Genetic Analysis of Host-Pathogen Interactions

Contact Info

Product

Resources

About