Demonstration of species non-specificity of the effect of hypersensitivity pyrogen

Johanovsky, J

doi:10.1007/bf02877177

Cited by 3 publications

(2 citation statements)

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“…In a series of publications, Johanovsk)~ has presented evidence that tuberculin (or PPD) reacts in vitro with specifically sensitized mononuclear cells from spleen or lymph nodes to produce a rapidly acting "hypersensitivity pyrogen." This pyrogen differed in several respects from both leukocytic and circulating endogenous pyrogens present in other experimental fevers (3)(4)(5)(6)(7)(8). Hypersensitivity pyrogen was produced when antigen was added to disrupted as well as to viable cells.…”

Section: Response Of Normal Rabbits Injected Intravenously With Mixtumentioning

confidence: 93%

“…In other experiments, various techniques employed by Johanovsk~ (3)(4)(5)(6)(7)(8) for producing so-called hypersensitivity pyrogen were followed as closely as possible according to the following protocols. (a) Cells were suspended in fresh serum from hypersensitive rabbits and incubated with tuberculin at 37°C for 18 hours.…”

Section: Incubation Of Tuberculin In Vitro Withmentioning

confidence: 99%

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Studies on Tuberculin Fever

Atkins

Heijn

1965

The Journal of Experimental Medicine

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Although fever is a well known and common manifestation of hypersensitivity, little has been learned about the sequence of events by which antigen disturbs temperature regulation in sensitized individuals. Using tuberculininduced fever in rabbits infected with BCG as a model, we have shown in previous papers that an endogenous pyrogen (EP) appears in the circulation of such animals during the febrile response (1, 2). When injected intravenously this agent causes immediate fevers in normal rabbits, and is, therefore, clearly distinct from tuberculin itself which produces fever after a latency of 45 to 60 minutes and is pyrogenic only in animals previously sensitized by BCG. The tissue source of EP in this form of hypersensitivity fever has remained uncertain. In several systems of hypersensitivity, including tuberculin, Johanovsk~ has reported that when antigen is added in vitro to mononuclear ceils from spleen or lymph nodes of specifically sensitized animals, a pyrogen is formed which he has called 'hypersensitivity pyrogen' to distinguish it from serum endogenous pyrogen present in other experimental fevers (3-8).In the following experiments, tuberculin was added in vitro to various tissues of BCG-sensitized rabbits in an attempt to repeat Johanovskg's findings and to determine the origin of the pyrogen which appears in the serum in tuberculin-induced fever. Johanovsk~'s observations were not confirmed, and evidence is presented instead that sensitized blood cells release EP in vitro when incubated with tuberculin and hence may be a source of the circulating pyrogen in this form of hypersensitivity fever.In additional experiments, it was found that normal blood cells, briefly incubated in plasma of sensitized animals, were similarly activated in vitro by tuberculin, an observation which suggests that humoral antibodies play a role in the genesis of tuberculin-induced fever.

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