Demonstration of sequential adaptation strategy for developing salt tolerance in bacteria for wastewater treatment: A study using Escherichia coli as model

Puranik, Sampada; Shaligram, Shraddha; Paliwal, Vasundhara; Raje, Dhananjay; Kapley, Atya; Purohit, Hemant J.

doi:10.1016/j.biortech.2012.06.037

Cited by 7 publications

(2 citation statements)

References 28 publications

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“…For the preparation of bacterial inoculum, the culture was grown up to a mid-exponential growth phase in nutrient broth and then, the grown bacterial culture was centrifuged, the cell pellets were washed and resuspended in water and adjusted to a cell density of 10 8 CFU mL −1 . The adaptation of A. brasilense NO40 for tolerance to high salt concentration was conducted through the sequential transfers approach with gradually increasing salt concentration in the culture media [13]. Overnight bacterial culture was inoculated in liquid media supplemented with 2% NaCl and incubated at 30 ± 2°C and 130 rpm for three days.…”

Section: Sequential Adaptation Approachmentioning

confidence: 99%

Azospirillum brasilense and Saccharomyces cerevisiae as Alternative for Decrease the Effect of Salinity Stress in Tomato (Lycopersicon esculentum) Growth

Abdelmoteleb¹,

González-Mendoza²,

Elbaalawy³

2022

Phyton

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The salinity stress is one of the most relevant abiotic stresses that affects the agricultural production. The present study was performed to study the improvement of the salt tolerance of tomato plants which is known for their susceptibility to salt stress. The present study aimed to assess to what extent strain Azospirillum brasilense (N040) and Saccharomyces cerevisiae improve the salt tolerance to tomato plants treated with different salt concentration. The inoculant strain A. brasilense (N040) was previously adapted to survive up to 7% NaCl in the basal media. A greenhouse experiment was conducted to evaluate the effect of this inoculation on growth parameter such as: plant height, root length, fresh and dry weight, fruits fresh weight, chlorophyll content, proline and total soluble sugar in tomato plants under salt stress condition. The results revealed that co-inoculation of Azospirillum brasilense (N040) and Saccharomyces cerevisiae significantly increased the level of proline (8.63 mg/g FW) and total soluble sugar (120 mg/g FW) of leaves under salinity condition comparing to non-inoculated plants (2.3 mg/g FW and 70 mg/g FW, respectively). Plants co-inoculated with adapted strain of A. brasilense and S. cerevisiae showed the highest significant (p < 0.01) increase in fruit yield (1166.6 g/plant), plant high (115 cm) and roots length (52.6) compared whit un-inoculated control plants (42 g/pant, 43.3 cm and 29.6 cm, respectively). In contrast, Na + ion content was significantly decreased in the leaves of salt stressed plants treated with the A. brasilense (N040) and S. cerevisiae. Finally, the results showed that dual benefits provided by both A. brasilense (N040) and S. cerevisiae can provide a major way to improve tomato yields in saline soils.

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Section: Sequential Adaptation Approachmentioning

confidence: 99%

Azospirillum brasilense and Saccharomyces cerevisiae as Alternative for Decrease the Effect of Salinity Stress in Tomato (Lycopersicon esculentum) Growth

Abdelmoteleb¹,

González-Mendoza²,

Elbaalawy³

2022

Phyton

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“…[3][4][5][6] The changing concentration of salt is one of the most common abiotic stress factors on the earth. 7 It thus comes as no surprise that TCSTSs have been identified involved in the adaptation to salt concentration in many bacteria, and some of these TCSTS systems, such as the DegS-DegU system in Bacillus subtilis, 8 the NtrY/NtrX system in Rhizobium tropici, 9 the KdpE/ RsbQ system in Listeria monocytogenes, 10 a response regulator a Laboratory of Synthetic Microbiology, School of Chemical Engineering & in a halophilic Chromohalobacter salexigens, 11 and CpxR/OmpR, KdpD/KdpE and OmpR-EnvZ systems in Escherichia coli, [12][13][14][15] have been well characterized.…”

Section: Introductionmentioning

confidence: 99%

An orphan two-component response regulator Slr1588 involves salt tolerance by directly regulating synthesis of compatible solutes in photosynthetic Synechocystis sp. PCC 6803

Chen

Zhu

et al. 2014

Mol. BioSyst.

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We report here the characterization of a novel orphan response regulator Slr1588 directly involved in the synthesis and transport of compatible solutes against salt stress. In the Δslr1588 mutant, salt tolerance was found to be decreased by 2-3-fold. Using a high performance Q-EXACTIVE hybrid quadrupole-Orbitrap mass spectrometer, we found that proteins involved in the synthesis and transport of glucosylglycerol, a key compatible solute, were up-regulated in the Δslr1588 mutant grown in 4.0% NaCl, suggesting that Slr1588 might function as a repressor for glucosylglycerol metabolism. The functional assignment was further confirmed using an electrophoretic mobility shift assay (EMSA) showing that the purified his-tagged Slr1588 could bind in vitro directly to the upstream regions of sll1566 (ggpS) genes required for glucosylglycerol biosynthesis. In addition, quantitative proteomic analysis showed that the biosynthesis of another key compatible solute in Synechocystis, sucrose, was also up-regulated in the Δslr1588 mutant under 4.0% NaCl, and EMSA showed that the purified his-tagged Slr1588 bound in vitro directly to the upstream regions of sll0045 (spsA) gene required for sucrose biosynthesis. Moreover, proteomic analysis showed that 113 and 127 unique proteins were and up- and down-regulated in the Δslr1588 mutant grown under 4.0% NaCl, respectively. Notably, a dozen transporter genes were down-regulated in the Δslr1588 mutant under salt stress. The study revealed a novel salt-tolerant regulatory mechanism mediated by Slr1588, and also provided a proteomic description of the possible Slr1588 regulon in Synechocystis.

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Dynamic interactive events in gene regulation using E. coli dehydrogenase as a model

Puranik

Purohit

2014

Funct Integr Genomics

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Different approaches in gene expression analysis always provide a snapshot view of cellular events. During the bacterial growth, the decisions are dynamically made with participation of various genes and their interactions with modulating factors. We have selected Escherichia coli dehydrogenases as a model to capture these interactions. We have treated the cells with hydrogen peroxide with very low level and asked the questions how cellular physiology has modulated itself to survive post-shock conditions. We hypothesized that while global regulators and associated gene network dictate the overall cellular intelligence, specific redox-sensitive classes of enzymes like dehydrogenase-mediated modulation could provide the option to cell for survival under peroxide after-effect. To understand the dynamic gene interaction, we used multidimensional scaling of genes and overlaid with minimum spanning tree to understand the clustering patterns under different conditions. Study shows that under peroxide after-effect, it is the interplay of ArcA (global regulator), with ldhA (involved in intermediary metabolism) and ndh (managing co-factor NADH), that emerges as modulating association. Knockout mutants of global regulators confirmed the promoter activity trend through gene expression change for dehydrogenases.

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Demonstration of sequential adaptation strategy for developing salt tolerance in bacteria for wastewater treatment: A study using Escherichia coli as model

Cited by 7 publications

References 28 publications

Azospirillum brasilense and Saccharomyces cerevisiae as Alternative for Decrease the Effect of Salinity Stress in Tomato (Lycopersicon esculentum) Growth

Azospirillum brasilense and Saccharomyces cerevisiae as Alternative for Decrease the Effect of Salinity Stress in Tomato (Lycopersicon esculentum) Growth

An orphan two-component response regulator Slr1588 involves salt tolerance by directly regulating synthesis of compatible solutes in photosynthetic Synechocystis sp. PCC 6803

Dynamic interactive events in gene regulation using E. coli dehydrogenase as a model

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