Demonstration of re-epithelialization in a bioprinted human skin equivalent wound model

Jara, Carlos Poblete; Catarino, Carolina Motter; Lei, Yu; Velloso, Lı́cio A.; Karande, Pankaj; Velander, William H.; Araujo, Eliana P.

doi:10.1016/j.bprint.2020.e00102

Cited by 13 publications

(10 citation statements)

References 25 publications

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“…They have been validated as alternative methods to animal models for the safety assessment of substances and explored as relevant tools for efficacy evaluation of topically applied products 54–57 . Furthermore, the field of regenerative medicine has evolved exponentially toward the development of skin grafts that could be used for clinical applications 27,58,59 . Nonetheless, engineered skin models still fail to recapitulate the complexity of the native tissue in terms of cellular diversity (e.g., lacking melanocytes, neural and immune cells), presence of adnexal structures (e.g., lacking hair follicle, sebaceous, and sweat glands), and vascularization.…”

Section: Discussionmentioning

confidence: 99%

“…We hypothesized that the inclusion of a DEJ layer in the skin reconstruction protocol could accelerate early keratinocyte proliferation and distribution contributing to the formation of a uniform confluent monolayer prior to the beginning of the cell differentiation process when exposed to the air‐liquid interface. Furthermore, we believe that this characteristic could contribute toward accelerating the host integration of skin graft placed in wounds as it is known that proliferation and migration of keratinocytes are fundamental for tissue reepithelization 58,66 . Considering this hypothesis and understanding that beyond cell attachment and cell proliferation rate, these DEJ proteins must promote proper keratinocyte differentiation, we evaluated the effect of four distinct protein compositions in the skin reconstruction context.…”

Section: Discussionmentioning

confidence: 99%

“… 54 , 55 , 56 , 57 Furthermore, the field of regenerative medicine has evolved exponentially toward the development of skin grafts that could be used for clinical applications. 27 , 58 , 59 Nonetheless, engineered skin models still fail to recapitulate the complexity of the native tissue in terms of cellular diversity (e.g., lacking melanocytes, neural and immune cells), presence of adnexal structures (e.g., lacking hair follicle, sebaceous, and sweat glands), and vascularization. Furthermore, the currently available reconstructed skin models employ a very limited array of matrix proteins and scaffold biomaterials compared to the diversity of biomolecules present in the human skin, which are important for tissue homeostasis.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, we believe that this characteristic could contribute toward accelerating the host integration of skin graft placed in wounds as it is known that proliferation and migration of keratinocytes are fundamental for tissue reepithelization. 58 , 66 Considering this hypothesis and understanding that beyond cell attachment and cell proliferation rate, these DEJ proteins must promote proper keratinocyte differentiation, we evaluated the effect of four distinct protein compositions in the skin reconstruction context. Following 14 days of tissue maturation, we did not observe any significant morphological differences between the control and test conditions.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of native and non‐native biomaterials for engineering human skin tissue

Catarino

Kaiser

Baltazar

et al. 2022

Bioengineering & Transla Med

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A variety of human skin models have been developed for applications in regenerative medicine and efficacy studies. Typically, these employ matrix molecules that are derived from non‐human sources along with human cells. Key limitations of such models include a lack of cellular and tissue microenvironment that is representative of human physiology for efficacy studies, as well as the potential for adverse immune responses to animal products for regenerative medicine applications. The use of recombinant extracellular matrix proteins to fabricate tissues can overcome these limitations. We evaluated animal‐ and non‐animal‐derived scaffold proteins and glycosaminoglycans for the design of biomaterials for skin reconstruction in vitro. Screening of proteins from the dermal‐epidermal junction (collagen IV, laminin 5, and fibronectin) demonstrated that certain protein combinations when used as substrates increase the proliferation and migration of keratinocytes compared to the control (no protein). In the investigation of the effect of components from the dermal layer (collagen types I and III, elastin, hyaluronic acid, and dermatan sulfate), the primary influence on the viability of fibroblasts was attributed to the source of type I collagen (rat tail, human, or bovine) used as scaffold. Furthermore, incorporation of dermatan sulfate in the dermal layer led to a reduction in the contraction of tissues compared to the control where the dermal scaffold was composed primarily of collagen type I. This work highlights the influence of the composition of biomaterials on the development of complex reconstructed skin models that are suitable for clinical translation and in vitro safety assessment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of native and non‐native biomaterials for engineering human skin tissue

Catarino

Kaiser

Baltazar

et al. 2022

Bioengineering & Transla Med

Self Cite

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“…Compared to the untreated control lesion that showed a less clear structure, more empty spaces, hair follicle damage and high numbers of neutrophilic inflammatory cells (Figure 5a), wounds treated with BC scaffolds showed increased healing effects, as indicated by the presence of keratinocytes and fewer granular cells or inflammatory cells after 7 days (Figure 5c). During re-epithelialization, fibroblasts are recruited to rebuild the dermal layer along with the migration of keratinocytes from the edges of the wound to re-epithelialize the surrounding matrix [43]. After 14 days of wound healing, in this study, an improved arrangement of collagen fibers, stratified squamous epithelium and dense newborn subcutaneous tissue, which integrated with the granular tissue in the epidermis, was displayed in wound tissue with BC scaffold treatment (Figure 5d), when compared with the untreated lesion control (Figure 5b).…”

Section: In Vivo Assessment Using a Rat Skin Defect Modelmentioning

confidence: 99%

Bacterial Cellulose as a Potential Bio-Scaffold for Effective Re-Epithelialization Therapy

et al. 2021

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Currently, there are several therapeutic approaches available for wound injury management. However, a better understanding of the underlying mechanisms of how biomaterials affect cell behavior is needed to develop potential repair strategies. Bacterial cellulose (BC) is a bacteria-produced biopolymer with several advantageous qualities for skin tissue engineering. The aim here was to investigate BC-based scaffold on epithelial regeneration and wound healing by examining its effects on the expression of scavenger receptor-A (SR-A) and underlying macrophage behavior. Full-thickness skin wounds were generated on Sprague-Dawley rats and the healing of these wounds, with and without BC scaffolds, was examined over 14 days using Masson’s trichome staining. BC scaffolds displayed excellent in vitro biocompatibility, maintained the stemness function of cells and promoted keratinocyte differentiation of cells, which are vital in maintaining and restoring the injured epidermis. BC scaffolds also exhibited positive in vivo effects on the wound microenvironment, including improved skin extracellular matrix deposition and controlled excessive inflammation by reduction of SR-A expression. Furthermore, BC scaffold significantly enhanced epithelialization by stimulating the balance of M1/M2 macrophage re-programming for beneficial tissue repair relative to that of collagen material. These findings suggest that BC-based materials are promising products for skin injury repair.

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CSMP: A Self‐Assembled Plant Polysaccharide‐Based Hydrofilm for Enhanced Wound Healing

Qiao,

Zhang,

Liu

et al. 2023

Adv Healthcare Materials

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Wound management remains a critical healthcare issue due to the rising incidence of chronic diseases leading to persistent wounds. Traditional dressings have their limitations, such as potential for further damage during changing and suboptimal healing conditions. Recently, hydrogel‐based dressings have gained attention due to their biocompatibility, biodegradability, and ability to fill wounds. Particularly, polysaccharide‐based hydrogels have shown potential in various medical applications. This study focuses on the development of a novel hydrofilm wound dressing produced from a blend of chia seed mucilage (CSM) and polyvinyl alcohol (PVA), termed CSMP. While the individual properties of CSM and PVA are well‐documented, their combined potential in wound management is largely unexplored. CSMP, coupled with sorbitol and glycerin, and cross‐linked using ultraviolet light, results in a flexible, adhesive, and biocompatible hydrofilm demonstrating superior water absorption, moisturizing, and antibacterial properties. This hydrofilm promotes epithelial cell migration, enhanced collagen production, and outperforms existing commercial dressings in animal tests. The innovative CSMP hydrofilm offers a promising, cost‐effective approach for improved wound care, bridging existing gaps in dressing performance and preparation simplicity. Future research can unlock further applications of such polysaccharide‐based hydrofilm dressings.

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Demonstration of re-epithelialization in a bioprinted human skin equivalent wound model

Cited by 13 publications

References 25 publications

Evaluation of native and non‐native biomaterials for engineering human skin tissue

Evaluation of native and non‐native biomaterials for engineering human skin tissue

Bacterial Cellulose as a Potential Bio-Scaffold for Effective Re-Epithelialization Therapy

CSMP: A Self‐Assembled Plant Polysaccharide‐Based Hydrofilm for Enhanced Wound Healing

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