Demonstration of leptospiral antigens on tissues using monoclonal antibodies and avidin-biotin peroxidase staining

Pereira, Martha María; Andrade, Juliana da Silva Ribeiro de; Lacerda, Maria Diana; Batoreu, Nadia Maria; Marchevsky, Renato Sérgio; Santos, Ricardo Ribeiro dos

doi:10.1016/s0940-2993(97)80155-8

Cited by 17 publications

(18 citation statements)

References 14 publications

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“…2). This staining pattern is similar to previous reports in both experimentally infected and naturally exposed species (Pereira et al, 1997;Yener and Keles, 2001;Wild et al, 2002), and might vary with chronicity of infection. The staining of antigen in all samples was most prominent in areas of interstitial inflammation.…”

supporting

confidence: 91%

Leptospirosis in Northern Elephant Seals (Mirounga angustirostris) Stranded along the California Coast

Colegrove

Lowenstine

Gulland

2005

Journal of Wildlife Diseases

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Leptospirosis was identified in six northern elephant seals (Mirounga angustirostris) that were stranded in 1995 along the coast of California (USA). Histologic lesions in all seals included tubulointerstitial nephritis with tubular degeneration and necrosis. Infection was confirmed through identification of spirochetes using an immunohistochemical stain for Leptospira sp. antigens. One affected seal had an elevated titer to Leptospira interrogans serovar pomona. Four of the six seals developed leptospirosis during rehabilitation, and two seals had evidence of exposure in the wild. Potential sources of infection during rehabilitation include other elephant seals, California sea lions (Zalophus californianus), Pacific harbor seals (Phoca vitulina richardsii), or free-ranging wildlife. These results indicate that northern elephant seals are susceptible to leptospirosis and can develop disease both in the natural environment and in a rehabilitation setting.

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supporting

confidence: 91%

Leptospirosis in Northern Elephant Seals (Mirounga angustirostris) Stranded along the California Coast

Colegrove

Lowenstine

Gulland

2005

Journal of Wildlife Diseases

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“…Other toxic effects exerted by GLP may be observed in cultures of Vero and L929 cells, leading to cell membrane perforations followed by cell death (43). The presence of GLP in damaged tissues of patients and experimental animals further supports the role of this molecule in leptospirosis (25,33).…”

Section: Discussionmentioning

confidence: 79%

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

et al. 2002

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“…12 Previous immunoperoxidase staining of renal histologic sections of hamsters with acute tubular necrosis caused by L. interrogans serovar canicola had reactivity for intact leptospires and granular deposits in perivascular locations. 20 Only the homologous serovar was agglutinated by monoclonal antibodies, indicating that the recognized epitope is a surface-exposed antigen. 20 Individual serovars may cause different expression of bacterial surface components during replication in vivo.…”

Section: Discussionmentioning

confidence: 98%

“…20 Only the homologous serovar was agglutinated by monoclonal antibodies, indicating that the recognized epitope is a surface-exposed antigen. 20 Individual serovars may cause different expression of bacterial surface components during replication in vivo. In vivo adaptation by pathogenic leptospires appears to involve a differential expression of outer membrane components, including proteins and LPS.…”

Section: Discussionmentioning

confidence: 98%

An Improved Immunohistochemical Diagnostic Technique for Canine Leptospirosis using Antileptospiral Antibodies on Renal Tissue

et al. 2002

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Abstract. The purpose of this study was to compare the immunoreactivity in canine renal tissues stained with antisera specific for 3 leptospiral antigens and those processed with traditional staining methods. In addition, immunoglobulin staining was done on tissues with immunoreactivity to leptospiral antigens. Formalinfixed renal sections from 12 dogs with chronic interstitial nephritis suspected or proven to have leptospirosis (6 dogs with silver-stained leptospires and 6 dogs in which silver-stained leptospires were not detected) were used. Antibodies consisted of a monoclonal antibody to Leptospira kirschneri serovar grippotyphosa lipopolysaccharide (LPS) and 2 polyclonal antibodies to outer membrane proteins, including OmpL1, a leptospiral porin, and LipL41, an outer membrane lipoprotein. The murine monoclonal antisera against LPS (F71C2-1) had the most abundant and consistent immunoreactivity. Immunoreactive areas were present in 6 of 6 sections positive by silver staining and included extracellular granular debris in intertubular areas, debris in macrophages, organisms in tubular lumina, and cytoplasmic granules in tubular epithelia. Antisera with specificity for the outer membrane proteins OmpL1 and LipL41 detected only intact organisms in tubular lumina. Immunoreactivity to OmpL1 (polyclonal 338) occurred in 4 of 5 sections positive by silver staining, but immunoreactivity to LipL41 (polyclonal 813) occurred in only 1 of 6 silver-positive sections. Each of the kidney sections in which leptospiral antigens were detected by immunohistochemistry also was positive by silver staining. Sections negative by silver staining were also negative by immunostaining. Although immunohistochemistry did not enhance sensitivity, amplification of signal by secondary antibody and hematoxylin counterstaining improved the ease of diagnosis and allowed better evaluation of tissue morphology than did silver staining methods. IgG was the most abundant immunoglobulin. IgG immunoreactivity occurred predominantly in plasma cells within interstitial infiltrates. Interstitial infiltrates contained abundant immunoreactivity to LPS, but immunoreactivity to OmpL1 and LipL41 was not noted.

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Demonstration of leptospiral antigens on tissues using monoclonal antibodies and avidin-biotin peroxidase staining

Cited by 17 publications

References 14 publications

Leptospirosis in Northern Elephant Seals (Mirounga angustirostris) Stranded along the California Coast

Leptospirosis in Northern Elephant Seals (Mirounga angustirostris) Stranded along the California Coast

Peripheral Blood Mononuclear Cell Activation Induced byLeptospira interrogansGlycolipoprotein

An Improved Immunohistochemical Diagnostic Technique for Canine Leptospirosis using Antileptospiral Antibodies on Renal Tissue

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