Demonstration of Islet-Cell Antibodies

Sewell, Herb F.; Smith, DavidI.; Willox, A; Barnes, CharlesA.; Matthews, J B

doi:10.1016/s0140-6736(80)90536-x

Cited by 7 publications

(2 citation statements)

References 2 publications

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“…In particular, recent reports indicate that islet cell antibodies are an important predictive marker of ongoing preclinical beta cell destruction. 14 In combination with serial assessments of sensitive indices of beta cell mass/function, like the early-phase insulin release to i.v. glucose, islet cell antibody assays are crucial for monitoring the natural history and chronology of the beta cell destructive process.…”

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confidence: 99%

Assay for Islet Cell Antibodies: Protein A—Monoclonal Antibody Method

et al. 1985

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SUMMARYAssays for islet cell antibodies (ICA) are finding increasing application in clinical diabetology. We have developed a new islet cell antibody assay system (ICA-pA), whose salient features include: (1) utilization of fluorescein-conjugated staphylococcal protein A as a standard second-step reagent, the advantages of this approach being improved "signal" (islet)/"noise" (acini) ratio due to reduction of interfering background acinar pancreatic staining, and facilitation of assay standardization provided by the use of a chemically pure conjugated protein A reagent; (2) monoclonal antibody counterstaining with rhodamine-conjugated BISL-32 for the rapid identification of islets in pancreatic sections; and (3) quantitation of circulating serum ICA by microimmunofluorometric techniques. DIABETES 1985; 34:300-305.A utoantibodies directed against the islet cells of the pancreas are an important hallmark of type I (insulin-dependent) diabetes mellitus. Besides, significance in research studies probing the mechanism(s) of autoimmune beta cell destruction, islet cell antibody assays are currently finding increasing application in clinical diabetology. In particular, recent reports indicate that islet cell antibodies are an important predictive marker of ongoing preclinical beta cell destruction.14 In combination with serial assessments of sensitive indices of beta cell mass/function, like the early-phase insulin release to i.v. glucose, islet cell antibody assays are crucial for monitoring the natural history and chronology of the beta cell destructive

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Assay for Islet Cell Antibodies: Protein A—Monoclonal Antibody Method

et al. 1985

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“…Antibodies reactive with determinants in the cytoplasm of pancreatic islet cells are found in many patients with newly diagnosed Type 1 (insulin-dependent) diabetes. These islet cell cytoplasmic antibodies (ICA) are detected with indirect immunofluorescence assays on sections of human blood group 0 pancreas [1][2][3][4]. Several investigators have studied the prevalence of islet cell antibodies in diabetes [5][6][7][8][9] among family members [10][11][12][13] in correlation with other autoimmune diseases [1,8,9,11,14,15] and their occurrence in the background population [15].…”

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Analysis of islet cell antibodies on frozen sections of human pancreas

Marner¹,

Lernmark²,

Nerup³

et al. 1983

Diabetologia

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The sensitivity and specificity of the assay for islet cell cytoplasmic antibodies in human serum were examined using cryostat sections from fresh frozen pancreas. The specificity of the assay was close to 100% while the sensitivity was 40%-98% depending on the pancreas used. Inter-observer variation was 12-27%. End-point titres of islet cell antibodies varied with the sensitivity of each pancreas. End-point titration of the antibodies in two different laboratories using the same pancreas was significantly correlated (Spearman test p less than 0.001). We conclude that a reliable determination of islet cell antibody titres in human serum requires careful characterization of the sensitivity and specificity of each pancreas used as a source of frozen sections, in the indirect immunofluorescence assay.

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