Demonstration of intermediate filaments in sheep ovary

Marettová, E.; Maretta, M.

doi:10.1078/0065-1281-00672

Cited by 27 publications

(23 citation statements)

References 10 publications

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“…These molecules act in the formation of cell contacts and in the determination of the cell morphology (Schliwa & Van Blerkom 1981, Luna & Hitt 1992. Changes in the intermediate filaments have also been observed in ovine normal ovaries, affecting the organization of the cytoskeleton of corpus luteum and atresic follicles (Marettova & Maretta 2002). In addition, studies have demonstrated that pharmacologic agents which destabilize the cytoskeletal proteins can affect considerably the production of steroid hormones (Chen et al 1994), determining important changes in the steroidogenesis and consequently in cystogenesis (Isobe et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have demonstrated that changes and destabilization of the cytoskeletal proteins (microtubules, microfilaments and intermediate filaments) of the ovary decrease hardly the production of steroid hormones and can contribute with the cystogenesis (Chen et al 1994). Although the expression of cytoskeletal proteins has been studied in normal ovarian structures of many species including the sheep (Marettova & Maretta 2002), cow (Van der Huck et al 1995) and rat (Selstam et al 1993), there are no investigations about the expression of these proteins in normal and affected swine ovary. Recently, it was suggested that cytoskeletal proteins conducting the functional and structural changes during the cystogenesis participated in the pathogenesis of the COD in rats ) and in cows (Ortega et al 2007b).…”

Section: Introductionmentioning

confidence: 99%

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Cytoskeletal proteins in the follicular wall of normal andcystic ovaries of sows

et al. 2015

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The expression of cytoskeletal proteins was evaluated immunohistochemically in 36 normal ovaries sampled from 18 sows and 44 cystic ovaries sampled from of 22 sows, was evaluated. All sows had history of reproductive problems, such as infertility or subfertility. The immunohistochemically stained area (IHCSA) was quantified through image analysis to evaluate the expression of these proteins in the follicular wall of secondary, tertiary, and cystic follicles. Cytokeratins (CK) immunoreactivity was strong in the granulosa cell layer (GC) and mild in the theca interna (TI) and externa (TE) of the normal follicles. There was severe reduction of the reaction to CK in the GC in the cystic follicles, mainly in the luteinized cysts. The immunoreactivity for vimentin was higher in the GC from normal and cystic follicles in contrast with the other follicular structures. In the luteinized cysts, the IHCSA for vimentin was significantly higher in TI and in both observed cysts, the labeling was more accentuated in TE. Immunohistochemical detection of desmin and α-SMA was restricted to the TE, without differences between the normal and cystic follicles. The results of the current study show that the development of ovarian cysts in sows is associated to changes in the expression of the cytoskeletal proteins CK and vimentin.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytoskeletal proteins in the follicular wall of normal andcystic ovaries of sows

et al. 2015

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“…They observed in antral follicles that vimentin reaction was mainly found in certain granulosa cell types that have been called atypical granulosa cells and that are considered markers of atresia [van den Hurk and Dijkstra, 1992;van den Hurk et al, 1992]. Marettova and Maretta [2002] showed in sheep ovaries a similar pattern of immunoreactivity for desmin and vimentin in the different follicles described by van den Hurk et al [1995]. Anderson and Lee [1997] showed in the POS rat model induced by dehydroepiandrosterone that the basal layer of granulosa cells loses its vimentin filaments and acquires keratin; they concluded that the basal layer of granulosa cells becomes transformed into an epithelium during cystogenesis and this is an example of mesenchymalepithelial transformation associated with this pathological process.…”

Section: Discussionmentioning

confidence: 99%

“…The cytoskeletal proteins have been extensively examined in the ovary cells of many species including laboratory animals, farm animals and humans [Santini et al, 1993;Selstam et al, 1993;van den Hurk et al, 1995;Khan-Dawood et al, 1996Pan and Auersperg, 1998;Löffler et al, 2000;Marettova and Maretta, 2002]. However, the changes that occur in pathological situations such as the development of ovarian cysts have been poorly studied.…”

Section: Introductionmentioning

confidence: 99%

Expression of Cytoskeletal Proteins in the Follicular Wall of Induced Ovarian Cysts

Salvetti

Gimeno

Lorente³

et al. 2004

Cells Tissues Organs

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Several experimental models have been developed for the study of the polycystic ovarian syndrome in the rat. In the present study, the syndrome was induced by exposure to constant light, and the expression of cytoskeletal proteins in the follicular wall was evaluated by immunohistochemistry. We analyzed the immunohistochemically stained area (IHCSA) by image analysis to evaluate the expression of intermediate filaments (vimentin, desmin, cytokeratins, gliofibrillary acidic protein and neurofilaments) and α-smooth muscle actin (α-SMA) in cystic ovaries in relation to normal ovaries. The granulosa cell layer of cystic follicles had a significantly greater IHCSA for vimentin than the normal antral follicles. This difference was also significant between atretic and antral follicles. Cytokeratins showed a very low expression in the granulosa cells of antral follicles of control ovaries while in granulosa cells of atretic and cystic follicles they showed a significantly higher IHCSA. Immunohistochemical localization of desmin and α-SMA was restricted to the theca externa. Immunoreactivity for gliofibrillary acidic protein and neurofilament was negative. The highest intensity in the staining with vimentin and cytokeratins observed in the granulosa cells of the cystic follicles is probably due to structural and functional changes that occur during the process of cystogenesis and they could be associated with intense changes in the expression of cytoskeletal proteins that may be essential to the proper cellular functioning.

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“…The expression of several classes of cytoplasmic IFs in the ovary has been described for several mammalian and avian species [Czernobilsky et al, 1985;Gall, 1991;Santini et al, 1993;Gallicano et al, 1994;Bukovsky et al, 1995;Ricken et al, 1995;van den Hurk et al, 1995;Pan and Auersperg, 1998;Löffler et al, 2000;Marettová and Maretta, 2002]. In some of these studies, vimentin and CKs were detected within the granulosa cells of follicles at various stages of growth and atresia, in luteal cells of the corpus luteum throughout the luteal phase, and in oocytes from both fetal and adult ovaries [Czernobilsky et al, 1985;Gall, 1991;Santini et al, 1993;Gallicano et al, 1994;van den Hurk et al, 1995;Marettová and Maretta, 2002].…”

Section: Introductionmentioning

confidence: 99%

Expression of Intermediate Filaments in the Balbiani Body and Ovarian Follicular Wall of the Japanese Quail <b><i>(Coturnix japonica)</i></b>

Rodler¹,

Sinowatz²

2013

Cells Tissues Organs

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In the present study, we examined the distribution of 6 groups of intermediate filaments (IFs; cytokeratins, CKs, vimentin, synemin, desmin, glial fibrillary acidic protein and lamins) in oocytes and follicular walls of the Japanese quail (Coturnix japonica) during their development using immunohistochemical and ultrastructural techniques. A distinctly vimentin- and synemin-positive Balbiani body, which is a transient accumulation of organelles (mitochondria, Golgi complex and endoplasmic reticulum) that occurs in the oocytes of all vertebrates including birds, could be detected in the oocytes of primordial and early pre-vitellogenic follicles. In larger pre-vitellogenic follicles, the Balbiani body has dispersed and the positivity of the granulosa cells appeared to concentrate in the basal portion of their cytoplasm. Our ultrastructural data demonstrated that the matrix of the Bal-biani body consists of fine IFs, which may play a role in the formation and dispersion of the Balbiani body. Of the CKs studied (panCK, CK5, CK7, CK8, CK14, CK15, CK18 and CK19), only CK5 showed a slight positive staining in both the theca externa and the Balbiani bodies of pre-vitellogenic oocytes. In conclusion, our data, which describe the changes in avian IF protein expression during folliculogenesis, suggest that the functions of the IFs (vimentin and synemin) of oocytes and follicular walls are not primarily mechanical but may be involved in the transient tethering of mitochondria in the area of the Balbiani body and in the gain of endocrine competence during the differentiation of granulosa cells.

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Demonstration of intermediate filaments in sheep ovary

Cited by 27 publications

References 10 publications

Cytoskeletal proteins in the follicular wall of normal andcystic ovaries of sows

Cytoskeletal proteins in the follicular wall of normal andcystic ovaries of sows

Expression of Cytoskeletal Proteins in the Follicular Wall of Induced Ovarian Cysts

Expression of Intermediate Filaments in the Balbiani Body and Ovarian Follicular Wall of the Japanese Quail <b><i>(Coturnix japonica)</i></b>

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