Demonstration of immunoreactive somatostatin-like substance in villi and decidua in early pregnancy

Kumasaka, T; Nishi, Nozomu; Yaoi, Y; Kido, Y; Saito, Motoi; Okayasu, Isao; Shimizu, Koïchi; Hatakeyama, Shigeru; Sawano, Shinji; Kokubu, Keiji

doi:10.1016/0002-9378(79)90793-2

Cited by 44 publications

(13 citation statements)

References 5 publications

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“…Since our immunohistochemical attempts failed to demonstrate the site of somatostatin production in porcine or rat ovaries, there may be a small possibility that ovarian somatostatin is produced by some sort of epithelial cells in the ovary resembling pancreatic D cells (Polak et al 1975), cytotrophoblasts of the human placenta (Watkins & Yen 1980;Kumasaka et al 1979) or parafollicular cells in the thyroid (Hökfelt et al 1975), though detectable level of SRIF by immunohistochemistry, which can be estimated approximately at 50 ng/g wet weight tissue, is far higher than that detectable by radio¬ immunoassay which can be estimated 1-2 ng/g wet weight tissue. Considering the reported existence of sympathetic nerves in bovine ovaries (Morimoto et al 1981), it is likely that ovarian somatostatin is derived from intra-ovarian noradrenergic neurons which are known to contain somatostatin-like immunoreactivity (Hökfelt et al 1977).…”

Section: Discussionmentioning

confidence: 97%

Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova

Mori

Irahara

Saito

et al. 1985

Acta Endocrinologica

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To investigate the physiological importance of somatotrophin release-inhibiting factor (SRIF), effects in vitro of synthetic SRIF 14 on germinal vesicle breakdown (GVB) of cultured porcine follicular ova were studied. The proportion of ova with GVB decreased gradually and significantly with increasing concentrations of SRIF 14 in the range from 6 \m=x\ 10\m=-\12 to 6 \m=x\ 10\m=-\7 m during a 22 h period of culture. The inhibitory effect was apparent for the period between 14 and 22 h in the course of culture but was reversed by a concomitant addition of anti-SRIF to the medium. Neither synthetic oxytocin, vasoactive intestinal polypeptide nor substance P exerted any inhibitory or stimulatory action on GVB. These results suggest a limited but definite inhibitory action of SRIF on GVB of porcine ova.Although the presence of somatotrophin releaseinhibiting factor (SRIF) in porcine ovaries has been demonstrated in our previous work (Mori et al. 1983), the physiological significance of this peptide is totally unknown at present. Oocyte meiosis inhi¬ bitor (OMI) present in porcine follicular fluid (Tsafriri et al. 1976) is very close to SRIF in molecular weight. However, these two bioactive peptides are different from each other, because SRIF was shown to be degraded readily by proteolytic enzymes in follicular fluid in our previous study, while OMI remains active. Since somatosta¬ tin interacts with calcium in insulin secretion (Taminato et al. 1975), and calcium may be in¬ volved in oocyte maturation (Wasserman & Masui 1976), we have attempted in the present work to examine the effects, if any, of somatostatin on meiosis of follicular ova of the same species. Materials and MethodsCollection and culture of cumulus-coated porcine ova Porcine ovaries from prepubertal gilts weighing approx¬ imately 100 kg were obtained at a local abbatoir and were transported to the laboratory in physiologic saline kept at 35°C. Follicular ova with cumulus cells were aspirated by needle puncture from medium-sized follicles of 2-5 mm in diameter, thoroughly rinsed with sterile physiological saline and were subjected to culture within 2 h of sacri¬ ficing the animals. Only ova with cumulus cells were used and the whole procedure was performed at 37°C.Ova were cultured after the method of Sato 8c Ishibashi (1977) for different periods at 37 ± 0.5°C in an incubator continuously flushed with 5% C02 and 95% air saturated with water, using modified Krebs-Ringer bicar¬ bonate solution, pH 7.2-7.4, as culture medium which was supplemented with 0.25 mM D-glucose, 25.0 mM sodium lactate, 1 mg/ml crystalline bovine serum albu¬ min fraction V (Armour, New York), 100 I U/ml penicil¬ lin and 50 µg/ml streptomycin. A set of 10 ova was pipetted into 0.4 ml culture medium which was placed in plastic Petri dish (4.5 cm in diameter), covered with light paraffin oil, which had been equilibrated with the gas phase in the incubator for 2 h prior to use.

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Section: Discussionmentioning

confidence: 97%

Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova

Mori

Irahara

Saito

et al. 1985

Acta Endocrinologica

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“…In pregnancy, a dramatic change in the release of various hormones is observed not only in the hypothalamo-pituitary-gonadal system but also in the placenta. In humans, the placenta produces numerous hormones and growth factors, including human CG (hCG), human placental lactogen (hPL), progesterone (P4), estrogens (estradiol, estrone, estriol, and estetrol) (32,33), specific pregnancy j31 glycoprotein (SP1) (34), renin (35), human chorionic corticotropin (36), human chorionic TSH (37), human chorionic FSH, human uterotropic placental hormone (38), placental LH-releasing factor (39), TSHreleasing factor (40), somatostatin-like substance (41), human nerve growth factor (42), epidermal growth factor (EGF) (43), TGFs (43,44), and insulin-like growth factors (IGFs) (45). Recently, it was found that the placenta also produces cytokines such as interleukin-1 and interleukin-6, which stimulate hCG secretion in trophoblast cells (46)(47)(48)(49)(50).…”

Section: A the Production Of Placental Hormones In Pregnancymentioning

confidence: 99%

Inhibin and Activin Production in Human Placenta

Qu¹,

Thomas²

1995

Endocrine Reviews

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“…Previous studies have described material with the immunologial properties of somatostatin in the human placenta (Nishihira & Yagihashi 1978;Kumasaka et al 1979;Etzrodt et al 1980). How¬ ever, the characterisation of this material is still uncertain.…”

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confidence: 95%

Identification of somatostatin in the human placenta

Lee

Chard

1982

Acta Endocrinologica

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High pressure liquid chromatography (HPLC), gel chromatography and radioimmunoassay (RIA) were applied to the identification of cyclic somatostatin in human placental tissue. Methanol extracts of the placenta were treated with acetic acid and chloroform, purified on an octadecylsilane column, fractionated on HPLC and gel filtration, and assessed by RIA. It is concluded that human placental somatostatin is similar or identical to cyclic somatostatin on other tissue.

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Demonstration of immunoreactive somatostatin-like substance in villi and decidua in early pregnancy

Cited by 44 publications

References 5 publications

Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova

Inhibitory action of somatostatin on meiotic maturation of cultured porcine follicular ova

Inhibin and Activin Production in Human Placenta

Identification of somatostatin in the human placenta

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