Demonstration of immunoproteins in araldite-embedded tissues.

Giddings, June; Griffin, Ronda; MacIver, A. G.

doi:10.1136/jcp.35.1.111

Cited by 14 publications

(2 citation statements)

References 10 publications

(1 reference statement)

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“…Cuando es necesaria la digestión enzimática con agentes proteolíticos, disponemos de diferentes enzimas, se destacan la tripsina, proteasa, pronasa, solución de pepsina, etc. (11,(14)(15) Posteriormente al tratamiento enzimático y antes de la aplicación de los anticuerpos, es necesario inhibir la actividad de la peroxidasa endógena mediante peróxido de hidrógeno, para evitar la tinción de fondo inespecífica. (16-17-18-19) Los cambios de dilución del anticuerpo primario a utilizar van a determinar variaciones en los resultados.…”

Section: Discussionunclassified

Diagnóstico del carcinoma escamoso de la mucosa bucal: reporte de 5 casos

Baudo

2005

Av Odontoestomatol

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It is certain that the cancer buccal cause an important number of deaths at world level. The cancer invasivo can remain long time low changes macroscopic unspecific, the biopsy only allows its diagnosis. The more used technique is the routine with hematoxylin-eosin, at the moment is added to the same others that contribute to clarify the diagnosis, for example the inmunohistoquímica that will determine the stock of a neoplasia in many cases.

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Section: Discussionunclassified

Diagnóstico del carcinoma escamoso de la mucosa bucal: reporte de 5 casos

Baudo

2005

Av Odontoestomatol

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“…The immunoperoxidase method can then be performed using a standard trypsin incubation time. Different trypsin times will be required for tissues fixed in other agents, for longer periods or for resin-embedded tissues (Giddings, Griffin & MacIver 1981).…”

Section: Disadvantages Of a N Immunofluorescence Technique O N Frozenmentioning

confidence: 99%

Immunoperoxidase techniques in human renal biopsy

MacIver

Mepham

1982

Histopathology

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Immunoperoxidase techniques can be easily performed on formalin fixed renal tissue after incubation in trypsin. The sensitivity of the PAP technique on fixed tissue is as good as immunofluorescence methods using frozen sections. The method provides permanent preparations with clear localization of deposited immunoproteins giving accurate classification of renal disease and assessment for treatment. The availability of this technique for retrospective and centralized studies is a major advantage. Infectious agents are rendered harmless and can be specifically identified. The development of the trypsin-immunoperoxidase technique is an important and exciting advance in renal immunopathology.

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Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections

Litwin

Yokota²,

Hashimoto

et al. 1984

Histochemistry

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A procedure is described for light microscopic immunocytochemical localization of catalase and three enzymes of peroxisomal lipid beta-oxidation: acyl-CoA oxidase, enoyl-CoA hydratase and 3-ketoacyl-CoA thiolase in semi-thin sections of rat liver processed for routine electron microscopy. Satisfactory immunostaining required the removal of the epoxy resin with sodium ethoxide, controlled digestion of deplasticized sections with proteases and, in case of osmiumfixed tissue, bleaching with oxidants. Resin removal was essential for successful immunostaining, and protease treatment enhanced markedly the intensity of the reaction. This study shows that tissues processed for conventional ultrastructural studies can be used for postembedding immunocytochemical demonstration of various peroxisomal enzymes.

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Demonstration of immunoproteins in araldite-embedded tissues.

Cited by 14 publications

References 10 publications

Diagnóstico del carcinoma escamoso de la mucosa bucal: reporte de 5 casos

Diagnóstico del carcinoma escamoso de la mucosa bucal: reporte de 5 casos

Immunoperoxidase techniques in human renal biopsy

Light microscopic immunocytochemical demonstration of peroxisomal enzymes in epon sections

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