Demonstration of immunoglobulin G receptors induced by human cytomegalovirus

Furukawa, Toru; Hornberger, Elizabeth; Sakuma, Satomi; Plotkin, Stanley А.

doi:10.1128/jcm.2.4.332-336.1975

Cited by 79 publications

(3 citation statements)

References 9 publications

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“…The antibody titer was determined as the highest serum dilution producing a 1+ specific nuclear fluorescence reaction with CMV-infected cells. Care was taken to avoid misinterpretation of nonspecific cytoplasmic inclusion body fluorescence (6). Standard CMV antibody-positive and -negative sera were included in each test as controls.…”

Section: Methodsmentioning

confidence: 99%

“…In comparative studies, the immunofluorescent-antibody (FA) test has exhibited greater sensitivity than the CF test (2,13,15). However, since tissue culture cells infected with CMV develop Fc receptors to which immunoglobulin G (IgG) from immune and nonimmune sera adheres, the specificity of the FA test has been questioned by Furukawa and co-workers (6). The earlier studies of the usefulness of the FA test (2, 13, 15) have not resolved the problem of false-positive serological results.…”

mentioning

confidence: 99%

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Comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection

Betts¹,

George²,

Rundell³

et al. 1976

J Clin Microbiol

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Three different tests for detection of antibodies to human cytomegalovirus (CMV), complement fixing with antigen prepared by freeze-thaw disruption (CF-FT) or with antigen prepared by extraction with alkaline glycine buffer (CF-GE) and immunofluorescent staining (FA), were compared in renal transplant recipients and their healthy donors, FA and CF-GE tests yielded positive results at an identical and significantly higher frequency than CF-FT in both donors and recipients. CF-GE and FA performed on donors and recipients predicted all virus shedding post-transplant, whereas CF-FT did not. In the individuals who developed primary infection concurrent with the transplanted kidney, FA developed earlier than other antibodies in about one-half and at the same time in the remainder. In addition, the FA test could be completed more quickly and all sera could be interpreted, which made the FA test more useful than the CF-GE, but both of these tests were clearly superior to CF-FT.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection

Betts¹,

George²,

Rundell³

et al. 1976

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…Several structurally unrelated viral IgG Fc-binding glycoproteins (vFcγRs) have been identified for different herpesviruses: the gE/gI glycoprotein complex encoded by herpes simplex virus (HSV)-1 genes US8 and US7 respectively (21)(22)(23), gE encoded by Varicella zoster virus (VZV) (24)(25)(26) as well as various glycoproteins examined across different CMV species (27)(28)(29)(30)(31)(32). The HCMV genome encodes four vFcγRs, RL11 (RL11 or gp34), UL119/118 (UL119/118 or gp68), RL12 (RL12 or gp95) and RL13 (RL13 or gpRL13) (29,33).…”

Section: Introductionmentioning

confidence: 99%

Rhesus Cytomegalovirus-encoded Fcγ-binding glycoproteins facilitate viral evasion from IgG-mediated humoral immunity

Otero,

Petkova,

Ebermann

et al. 2024

Preprint

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Human cytomegalovirus (HCMV) encodes four viral Fc-gamma receptors (vFcγRs) that counteract antibody-mediated activationin vitro, but their role in infection and pathogenesis is unknown. To examine thein vivofunction of vFcγRs in animal hosts closely related to humans, we identified and characterized vFcγRs encoded by rhesus CMV (RhCMV). We demonstrate that Rh05, Rh152/151 and Rh173 represent the complete set of RhCMV vFcγRs, each displaying functional similarities to their respective HCMV orthologs with respect to antagonizing host FcγR activationin vitro. When RhCMV-naïve rhesus macaques were infected with vFcγR-deleted RhCMV, peak plasma viremia levels and anti-RhCMV antibody responses were comparable to wildtype infections. However, the duration of plasma viremia was significantly shortened in immunocompetent, but not in CD4+ T cell-depleted animals. Since vFcγRs were not required for superinfection, we conclude that vFcγRs delay control by virus-specific adaptive immune responses, particularly antibodies, during primary infection.

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Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Torfason

Källander

Halonen

1981

Journal of Medical Virology

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A radioimmunoassay (RIA) using polystyrene beads as the solid phase for cytomegalovirus (CMV) antigen and iodinated immunosorbent purified anti-human IgG, IgM, and IgA as indicator antibodies was developed for the detection of immunoglobulin class-specific antibodies to CMV. An antigen prepared from extracellular virus was essential for reliable results, and a preparation ultracentrifuged and sonicated twice was better than a crude antigen. The optimal antigen gave low cpm values with a negative reference serum, resulting in cpm ratios of 10 or higher between early convalescent phase serum and negative reference serum. Of six patients with an increase in CMV CF titres, all six had an increase in RIA IgG titres, four had an increase in IgA titres, and all had IgM antibodies. The IgG titres were high, up to 1/64,000. In a group of 17 infants negative in CMV CF test, 14 had CMV IgG antibodies in RIA test, indicating mainly low levels of maternal antibodies. In six of seven patients with CMV isolations from urine specimens, an increase in IgG or IgA titres or the presence of IgM antibodies was found, and only one of these patients had an increase in CMV CF titre. The specificity of the developed CMV RIA test was further demonstrated by detecting no significant increase in RIA titres in serum specimens of patients with primary herpes simplex infection, chickenpox, herpes zoster, or infectious mononucleosis.

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Demonstration of immunoglobulin G receptors induced by human cytomegalovirus

Cited by 79 publications

References 9 publications

Comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection

Comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection

Rhesus Cytomegalovirus-encoded Fcγ-binding glycoproteins facilitate viral evasion from IgG-mediated humoral immunity

Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

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