1978
DOI: 10.1073/pnas.75.7.3090
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Demonstration of high-affinity folate binding activity associated with the brush border membranes of rat kidney.

Abstract: Folate binding activity of high affinity was identified in the particulate fractions of rat kidney homogenates. This binding activity cofractionated with alkaline phosphatase and maltase, two brush border membranes markers. With an enriched preparation of brush border membranes, freed of endogenous folate by acid treatment, the binding of [3Hlfolate was found to be saturable (Kb = 4.2 X 10-11 M) and rapid. Binding was optimal at pH 6. 4-7.7 [3H]folic acid into the left renal artery of dogs, 6-24% of the 3H wa… Show more

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Cited by 36 publications
(13 citation statements)
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References 28 publications
(22 reference statements)
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“…Injection of 3 H-MTHF also confirms the important role of folbp1 for renal tubular reabsorption, showing increased urinary excretion and decreased renal accumulation in folbp1Ϫ/Ϫ. These findings, establishing for the first time in an in vivo model a direct role for folbp1 in epithelia folate uptake, confirms the hypotheses raised by the identification of FR in the kidney proximal tubule and by observations on folate transport in the kidney and in cultured kidney cells (14,20,32,33). In addition, folbp1 is heavily expressed in other absorptive epithelia, including the rodent yolk sac (34) and choroid plexus (15).…”
Section: Discussionsupporting
confidence: 77%
“…Injection of 3 H-MTHF also confirms the important role of folbp1 for renal tubular reabsorption, showing increased urinary excretion and decreased renal accumulation in folbp1Ϫ/Ϫ. These findings, establishing for the first time in an in vivo model a direct role for folbp1 in epithelia folate uptake, confirms the hypotheses raised by the identification of FR in the kidney proximal tubule and by observations on folate transport in the kidney and in cultured kidney cells (14,20,32,33). In addition, folbp1 is heavily expressed in other absorptive epithelia, including the rodent yolk sac (34) and choroid plexus (15).…”
Section: Discussionsupporting
confidence: 77%
“…This BBM:homogenate ratio for typical BBM enzymes y-glutamyltransferase and maltase was 25.0 and 18.0, respectively; for basolateral plasma membranie marker (Na+-K+) ATPase, the ratio was 0.5; for mitochondrial marker enzymes cytochrome c oxidase and succinate dehydrogenase, the ratio was 0.07 and 0.06, respectively. These results are similar to those obtained by others (18)(19)(20) and indicate high purity of BBM fraction.…”
supporting
confidence: 82%
“…Two folate transport proteins have been identified in kidney tissue: the reduced folate carrier (RFC) and the folate receptor (FR) (27,33,49,51). The RFC is found on the basolateral surface of kidney proximal tubule cells (55), while FRs are found on the apical brush-border membrane (3,12,22,24,49).…”
mentioning
confidence: 99%