Demonstration of glyoxalase II in rat liver mitochondria. Partial purification and occurrence in multiple forms

Talesa, Vincenzo; Uotila, Lasse; Koivusalo, Martti; Principato, Giovanni; Giovannini, Elvio; Rosi, Gabriella

doi:10.1016/0167-4838(88)90183-5

Cited by 49 publications

(32 citation statements)

References 18 publications

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“…The mechanism proposed for the glyoxalase IIcatalysed reaction involves an active site histidine residue. (1984, 1985, 1987a,b), Talesa et al (1988Talesa et al ( , 1989, Uotila (1973Uotila ( , 1979 (Ball & Vander Jagt, 1981). This is in keeping with the nucleophilicity of the imidazole group and the reactivity of N-acylimidazoles towards hydrolysis (Hall et al, 1978;Jencks & Carriuolo, 1959) (Fig.…”

Section: Glyoxalase IIsupporting

confidence: 66%

“…Enzymic activity of glyoxalase II is found throughout biological life. It is found in cell cytosols and in mitochondria but its presence in other organelles is less well-established (Jerzykowski et al, 1978;Talesa et al, 1988;Uotila, 1989).…”

Section: Glyoxalase IImentioning

confidence: 99%

“…In rat liver, 10-15 % of total glyoxalase II activity is in the mitochondria. The cytosolic enzyme consists of two forms of molecular mass 17 and 29 kDa, and pI values between 7.4 and 8.1 (Principato et al, 1987b;Talesa et al, 1988) (Table 2).…”

Section: Glyoxalase IImentioning

confidence: 99%

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The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life

Thornalley

1990

Biochemical Journal

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502

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Section: Glyoxalase IIsupporting

confidence: 66%

Section: Glyoxalase IImentioning

confidence: 99%

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The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life

Thornalley

1990

Biochemical Journal

728

502

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“…In addition to yeast, mitochondrial glyoxalase II activity has been detected in the tissues of higher eukaryotes including rat (9) and Arabidopsis thaliana (10). The function of the mitochondrial pool of glyoxalase II activity is unknown.…”

mentioning

confidence: 99%

“…The function of the mitochondrial pool of glyoxalase II activity is unknown. Of interest, glyoxalase I appears to be absent from mitochondria in the rat (9,11), however, S-D-lactoylglutathione has been detected in the mitochondria (12) and so may ultimately provide a substrate for glyoxalase II.…”

mentioning

confidence: 99%

The Human Hydroxyacylglutathione Hydrolase (HAGH) Gene Encodes Both Cytosolic and Mitochondrial Forms of Glyoxalase II

Cordell¹,

Futers

Grant

et al. 2004

Journal of Biological Chemistry

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In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II. In contrast, although glyoxalase II activity has been detected both in the cytosol and mitochondria of mammals, only a single gene encoding glyoxalase II has been identified. Previously it was thought that this gene (the hydroxyacylglutathione hydrolase gene), comprised 8 exons that are transcribed into mRNA and that the resulting mRNA species encoded a single cytosolic form of glyoxalase II. Here we show that this gene gives rise to two distinct mRNA species transcribed from 9 and 10 exons, respectively. The 9-exon-derived transcript encodes two protein species: mitochondrially targeted glyoxylase II, which is initiated from an AUG codon in a previously uncharacterized part of the mRNA sequence, and cytosolic glyoxalase II, which is initiated by internal ribosome entry at a downstream AUG codon. The transcript deriving from 10 exons has an in-frame termination codon between the two initiating AUG codons and hence only encodes the cytosolic form of the protein. Confocal fluorescence microscopy indicates that the mitochondrially targeted form of glyoxalase II is directed to the mitochondrial matrix. Analysis of glyoxalase II mRNA sequences from a number of species indicates that dual initiation from alternative AUG codons is conserved throughout vertebrates.

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Glyoxalase II inSaccharomyces cerevisiae: In SituKinetics Using the 5,5′-Dithiobis(2-nitrobenzoic Acid) Assay

Martins

Cordeiro

Freire

1999

Archives of Biochemistry and Biophysics

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Demonstration of glyoxalase II in rat liver mitochondria. Partial purification and occurrence in multiple forms

Cited by 49 publications

References 18 publications

The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life

The glyoxalase system: new developments towards functional characterization of a metabolic pathway fundamental to biological life

The Human Hydroxyacylglutathione Hydrolase (HAGH) Gene Encodes Both Cytosolic and Mitochondrial Forms of Glyoxalase II

Glyoxalase II inSaccharomyces cerevisiae: In SituKinetics Using the 5,5′-Dithiobis(2-nitrobenzoic Acid) Assay

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