Demonstration of Equine Herpes Virus 1 (EHV‐1) in Histological Sections and Tissue Cultures by the Peroxidase‐Antiperoxidase (PAP) Technique

Gimeno, Eduardo Juan; Nosetto, Edgardo Omar; Martín, A. Aranaz; Galosi, Cecília Mônica; Ando, Yoshinobu; Etcheverrigaray, María Elisa

doi:10.1111/j.1439-0450.1987.tb00455.x

Cited by 11 publications

(6 citation statements)

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“…In accordance with the findings of other authors (Whitwell, 1982;Gimeno et al, 1987;Edington et al, 1991), antigen detection was found to be slightly more sensitive than VI in this study, as in two cases virus could be detected only by IH. The primary site of virus replication is the fetal lung (Westerfield and Dimock, 1946).…”

Section: Discussionsupporting

confidence: 81%

“…Because they cannot be considered diagnostic for the virus infection, diagnosis should be supported by a specific laboratory test (Schultheiss et al, 1993). The VI technique is time consuming and expensive; additionally, false-negative results may be caused by post-mortem changes (Gimeno et al, 1987;Galosi et al, 2001). IH is relative cheap, fast and seems to be more sensitive than the VI method.…”

Section: Acta Veterinaria Hungarica 51 2003mentioning

confidence: 99%

“…The technique seems to be as sensitive as the indirect fluorescent antibody test in fetal organs (Schröer et al, 2000). The immunohistochemical (IH) method is another useful tool for detecting the virus in tissue samples (Gimeno et al, 1987). This technique enables detection of the virus and the histological changes at the same time.…”

mentioning

confidence: 99%

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Comparison of methods for the diagnosis of equine herpesvirus type 1 infection

Szeredi¹,

Pálfi²,

Molnár³

2003

Acta Veterinaria Hungarica

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The objective of the investigations was to study the occurrence of the equine herpesvirus type 1 (EHV-1) infection in aborted equine fetuses and in newborn foals and to compare the sensitivity of virus isolation, immunohistochemistry and histology in 101 cases and of fetal serology in 68 cases in the diagnosis of the infection. Out of the 93 aborted equine fetuses and 8 weak foals, 15 (14.9%) (14 fetuses and 1 foal) proved to be EHV-1 infected by immunohistochemical and 13 (12.9%) by virological investigation. Characteristic microscopic changes were seen in several organs in all cases, while intranuclear inclusion bodies could be found only in 25 (35.2%) of the 71 virus-positive tissue samples. Four (5.9%) cases proved to be positive by fetal serological investigation, but none of these cases showed any EHV-1 specific lesions and in none of these cases could the virus be detected by virus isolation or by immunohistochemistry. According to the results, fetal serology does not seem to be a useful test in viruspositive cases, while the immunohistochemical method seems to be a reliable and a slightly more sensitive method than virus isolation in the diagnosis of EHV-1 infection.

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Section: Discussionsupporting

confidence: 81%

Section: Acta Veterinaria Hungarica 51 2003mentioning

confidence: 99%

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Comparison of methods for the diagnosis of equine herpesvirus type 1 infection

Szeredi¹,

Pálfi²,

Molnár³

2003

Acta Veterinaria Hungarica

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“…The diagnosis in Argentina of EHV‐1 infection associated with abortion in mares has traditionally been based on the presence of histopatological changes and VI in cell culture (Gimeno et al., 1987). In this study, it was demonstrated that the PCR method described is rapid, sensitive and specific.…”

Section: Discussionmentioning

confidence: 99%

A Polymerase Chain Reaction for Detection of Equine Herpesvirus‐1 in Routine Diagnostic Submissions of Tissues from Aborted Foetuses

Galosi

Roza

Oliva

et al. 2001

Journal of Veterinary Medicine, Series B

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Equine herpesvirus 1 (EHV-1) is the causative agent of abortion, perinatal foal mortality, neurological and acute respiratory diseases in horses. Conventional laboratory diagnosis involving viral isolation from aborted foetuses is laborious and lengthy and requires processing of samples within 24 h of collection, which is problematic for samples that come from long distances. The aim of this study was to develop a polymerase chain reaction (PCR) assay useful in Argentina to detect DNA sequences of EHV-1 in different tissues from aborted equine foetuses with variable quality of preservation and without the use of conventional DNA fenolic extraction. Several DNA extraction protocols and primers were evaluated. The amplification method was standardized and its specificity was analysed using 38 foetal samples of variable quality of preservation. Of the 38 different foetal tissues, nine livers, six spleens and two lungs in good preservation and eight livers, one spleen and four lungs in a poor state of preservation were positive for PCR. EHV-1 was recovered only from the nine livers, five spleens and two lungs in good preservation. No virus was isolated from the samples that were poorly preserved. Viral isolation was confirmed by cytopathic effect and indirect immunofluorescence. The specificity of the PCR results was confirmed by the restriction endonuclease digestion of PCR products and hybridization.

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“…Routine clinicopathology may reveal leucopenia (Paradis 1996). A virus-specific diagnosis is made through virus culture, specific antibody titre .increaae (seroconversion) and detection of antigen through immunofluorescence, immunocytochemistry (Merkel and Zimmer 1981;Mumford 1984;Allen and Bryans 1986;Gimeno et at. 1987;van Maanen et at.…”

Section: Laboratory Diagnostic Aidsmentioning

confidence: 99%