Demonstration of epidermal transfer from a polymer membrane using genetically marked porcine keratinocytes

Grant, I. S.; Ng, Roy L.H; Woodward, B.; Bevan, Steve; Green, Colin; Martin, Robert H.

doi:10.1016/s0305-4179(00)00071-1

Cited by 20 publications

(9 citation statements)

References 26 publications

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“…Previously, keratinocytes have been delivered to “wound beds” in vitro and in vivo using fibrin glue18 and polymer sheets (for a recent example, see Ref. 19). These have relied upon initial keratinocyte attachment to an animal‐derived material (cell—e.g., fibroblast; or ECM protein—e.g., collagen).…”

Section: Discussionmentioning

confidence: 99%

Plasma‐polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound‐bed model

Haddow

Steele

Short

et al. 2002

J Biomedical Materials Res

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The aim of this study was to develop plasma-polymerized surfaces suitable for the attachment and culture of human keratinocytes and that would allow their subsequent transfer to a wound-bed model. Keratinocyte attachment has been assessed on a carrier polymer, either untreated or treated with a hydrocarbon plasma polymer, collagen I, or carboxylic-acid-containing plasma copolymers. Cell attachment was poor on the "bare" carrier polymer and hydrocarbon plasma polymer (PP) surfaces. Cell attachment was good and comparable on collagen I-coated carrier polymer and carrier polymer plasma coated with carboxylic acid functionalities. After 24 h of cell culture, surfaces were inverted so that cells were adjacent to a de-epidermalized dermis (DED) for 4 days. After 4 days in contact with DED, the surfaces were removed and the level of residual cells and cells transferred to DED were assessed using a cell viability assay. Cell transfer from the collagen I-coated surface was on the order of 90%. Transfer from the carrier polymer surface and the hydrocarbon-coated surface was poor while cells cultured on acid-containing surfaces showed high levels of transfer. Cell transfer was greatest from those surfaces containing the highest level of acid functionality (ca. 21%). Cell transfer was not significantly affected by the choice of carrier polymer material although some sample-to-sample variation was seen. To determine that plasma-polymerized surfaces could be used clinically, selected samples were sterilized with ethylene oxide. Subsequent analysis and cell culture indicated that the surface chemistry and cell-transfer capability of these plasma-polymerized surfaces were unaffected by the sterilization procedure. Plasma-polymerized carboxylic-acid-containing surfaces show great promise in the field of wound healing, encouraging keratinocyte attachment and permitting keratinocyte transfer to a wound bed.

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Section: Discussionmentioning

confidence: 99%

Plasma‐polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound‐bed model

Haddow

Steele

Short

et al. 2002

J Biomedical Materials Res

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“…It was found that HaCaT cell growth was stimulated by PETIM_0. 6. Cells reached earlier confluence on this substratum, while their growth was inhibited on a PEI membrane modified with PETIM_60, which makes PEI membranes modified with PETIM_0.6 a promising material for in vitro culture of epidermal transplants.…”

Section: Introductionmentioning

confidence: 98%

“…These measurements also indicated the presence of a swollen surface layer in the case of PETIM_60, while a regular structuring of the surface was observed with scanning force microscopy for PETIM_0. 6. A human keratinocyte cell line HaCaT was cultured on the different membranes.…”

Section: Introductionmentioning

confidence: 99%

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Poly(ether imide) Membranes Modified with Poly(ethylene imine) as Potential Carriers for Epidermal Substitutes

Trimpert

Boese

Albrecht

et al. 2006

Macromolecular Bioscience

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Poly(ether imide) (PEI) membranes were modified with a linear low-molecular weight (PETIM_0.6) and a branched high-molecular weight poly(ethylene imine) (PETIM_60). The membrane surfaces became more hydrophilic and the zeta potentials were shifted from negative to positive zeta values after immobilisation of both PETIM. These measurements also indicated the presence of a swollen surface layer in the case of PETIM_60, while a regular structuring of the surface was observed with scanning force microscopy for PETIM_0.6. A human keratinocyte cell line HaCaT was cultured on the different membranes. It was found that HaCaT cell growth was stimulated by PETIM_0.6. Cells reached earlier confluence on this substratum, while their growth was inhibited on a PEI membrane modified with PETIM_60, which makes PEI membranes modified with PETIM_0.6 a promising material for in vitro culture of epidermal transplants.

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“…Such stratification is not unusual for a living skin equivalent model of re-epithelialization [Garlick and Taichman, 1994]; however, it does show remodeling of the DEJ after the process of re-epithelialization in vitro. Other models which show such morphology are in vivo models where the epidermis has been grafted to a host animal [Grant et al, 2001].…”

Section: Discussionmentioning

confidence: 99%

The effect of induced biphasic pulsed currents on re‐epithelialization of a novel wound healing model

Bullock

Barker

Coulton

et al. 2006

Bioelectromagnetics

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The coordinated migration of keratinocytes is crucial to cutaneous wound healing; failure of keratinocytes to migrate into a wound can lead to chronic non-healing wounds. Keratinocyte migration can be influenced by applied electrical fields. Our aim was to investigate whether keratinocyte migration could be accelerated by applying an induced biphasic pulsed electrical field. We developed two in vitro biological systems models for this purpose: a keratinocyte colony-forming model and a reconstituted skin wound healing model with biphasic pulsed currents. Our in vitro skin models were capable of generating trans-epithelial potentials (TEP) similar to in vivo mammalian skin. Histological examination of the wound healing model also indicated that re-epithelialization occurred in a similar manner to that seen in vivo, although no evidence of a reconstitution of a basement membrane was seen during the 14 days in vitro experimental period. We found that growth of keratinocyte colonies and keratinocyte migration in an in vitro wound bed were not significantly affected by induced short duration biphasic pulsed currents at a frequency of 0.5 Hz of 100 and 200 mV/mm.

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Demonstration of epidermal transfer from a polymer membrane using genetically marked porcine keratinocytes

Cited by 20 publications

References 26 publications

Plasma‐polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound‐bed model

Plasma‐polymerized surfaces for culture of human keratinocytes and transfer of cells to an in vitro wound‐bed model

Poly(ether imide) Membranes Modified with Poly(ethylene imine) as Potential Carriers for Epidermal Substitutes

The effect of induced biphasic pulsed currents on re‐epithelialization of a novel wound healing model

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