Demonstration of Circulating Antibodies to Neurophysin in Patients Treated With Pitressin

Martin, Marion

doi:10.1677/joe.0.0490553

Cited by 15 publications

(6 citation statements)

References 6 publications

(6 reference statements)

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“…As has been reported previously (Martin, 1971), all but one of the Pitressintreated patients developed antibodies to neurophysin. Antibody titres in both rabbit and human antisera covered a wide range, but all possessed essentially the same maximum binding capacity in antibody excess, where antibody levels were sufficiently high for such a value to be recorded.…”

Section: Chromatoelectrophoresis and Maximum Binding Studies Onsupporting

confidence: 81%

The Development of a Radioimmunoassay for Bovine Neurophysin

Martin¹,

Chard²,

Landon³

1972

Journal of Endocrinology

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A radioimmunoassay for bovine neurophysin is described. The assay has a lower limit of detection of approximately 0\m=.\5 ng/ml. Bovine neurophysin extracted and purified from acetone-dried posterior pituitary lobe powder was used for standardization, radio-iodination and immunization of rabbits. Some anti-neurophysin sera were also obtained from human subjects treated with Pitressin. Most of the antisera tested did not readily distinguish between the different neurophysin components isolated from bovine neurohypophysial tissue. The antisera cross-reacted with material extracted from whole pituitaries of a number of mammalian species, including man, pig, sheep, guinea-pig and rat, but not with the pituitary peptides from the cod or dogfish, or the egret. Other pituitary peptides, including oxytocin, vasopressin, corticotrophin, growth hormone and luteinizing hormone, did not interfere with the assay.

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Section: Chromatoelectrophoresis and Maximum Binding Studies Onsupporting

confidence: 81%

The Development of a Radioimmunoassay for Bovine Neurophysin

Martin¹,

Chard²,

Landon³

1972

Journal of Endocrinology

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“…), until recently injections of Pitressin Tannate (vasopressin tannate) in oil have been the only satisfactory method for the treatment of severe cases. Pitressin, however, is an impure preparation which includes several peptides other than vasopressin, including oxytocin, (Martin, 1971), prolactin, and a corticotrophin-like peptide (Scott et al, 1972). Martin (1971) showed that patients treated with Pitressin may produce high titres of antibodies against neurophysin.…”

Section: Introductionmentioning

confidence: 99%

“…Pitressin, however, is an impure preparation which includes several peptides other than vasopressin, including oxytocin, (Martin, 1971), prolactin, and a corticotrophin-like peptide (Scott et al, 1972). Martin (1971) showed that patients treated with Pitressin may produce high titres of antibodies against neurophysin. Furthermore, patients often find the injections difficult to administer and unpleasant to receive.…”

Section: Introductionmentioning

confidence: 99%

Vasopressin Analogue DDAVP in Diabetes Insipidus: Clinical and Laboratory Studies

Edwards¹,

Kitau²,

Chard³

et al. 1973

BMJ

131

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“…J.) showed a significant binding of '251-NSN as well as 125I-ESN, which suggests that the patients were immunized with bovine or porcine neurophysin contaminated in the vasopressin tannate preparation (11). In Fig.…”

Section: Methodsmentioning

confidence: 84%

Neurophysin biosynthesis in vitro in oat cell carcinoma of the lung with ectopic vasopressin production.

Yamaji¹,

Ishibashi²,

Katayama³

et al. 1981

J. Clin. Invest.

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A B S T R A CAfter short labeling periods, most ofthe radioactivity resided in 20K species, which plateaued after 1 h, whereas 10K neurophysin progressively increased in its height. When cells were chased with unlabeled cysteine after the exposure to a short pulse of labeling, 20K neurophysin peak gradually decreased with an apparent initial half-life of 1 h. In contrast, the label in 10K neurophysin steadily increased, which exceeded the former by 3 h of chase. Analysis of 20K neurophysin in cell extract by isoelectric focusing on polyacrylamide gel demonstrated that it was principally composed of a protein with an apparent isoelectric point (pI) of 5.7.

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Demonstration of Circulating Antibodies to Neurophysin in Patients Treated With Pitressin

Cited by 15 publications

References 6 publications

The Development of a Radioimmunoassay for Bovine Neurophysin

The Development of a Radioimmunoassay for Bovine Neurophysin

Vasopressin Analogue DDAVP in Diabetes Insipidus: Clinical and Laboratory Studies

Neurophysin biosynthesis in vitro in oat cell carcinoma of the lung with ectopic vasopressin production.

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