Demonstration of an Internal Structure within the Red Blood Cell by Ion Etching and Scanning Electron Microscopy

Lewis, S. M.; Osborn, J S; Stuart, P. R.

doi:10.1038/220614a0

Cited by 51 publications

(13 citation statements)

References 6 publications

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“…Ion beam techniques have long been used for material removal in biological materials to 'etch' away surface layers of the targeted material by bombarding it with ionized gas molecules. This has been utilized to enhance the underlying microstructure for highly detailed structural observations (e.g., Lewis et al, 1968;Kanaya et al, 1982;Yonehara et al, 1989) and also in studies of dentin-adhesive interfaces (e.g., Van Meerbeek et al, 1992;Inokoshi et al, 1993a,b) and dentin restorative systems (e.g, Inokoshi et al, 1993a,b). Most of these studies have employed an ion beam from a gas discharge of an inert gas (typically argon), though more recently, 'focused' ion beams (FIB) that utilize a liquid metal (typically gallium) source (Orloff et al, 2003) to produce a probe of small diameter, now close to 10 nm (Van Es et al, 2004), are increasingly being used in large number of applications such as metrology, inspection, cross sectioning, failure analysis, mask-less micromachining, and preparation of thin foils for TEM (Reyntjens and Puers, 2001).…”

Section: Introductionmentioning

confidence: 98%

Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy

Nalla

Porter

Daraio

et al. 2005

Micron

103

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Section: Introductionmentioning

confidence: 98%

Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy

Nalla

Porter

Daraio

et al. 2005

Micron

103

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“…Etchpits and valleys and hillock and conical protuberances (Fulker et al, 1973;Lewis et al, 1968) have been observed, and there is evidence that some of the microscopic effects closely relate to the boundaries of anisotropic distribution of dS(v,O)ldO, which are represented by the angle of O1 and 02.…”

Section: Ion-beam Thinningmentioning

confidence: 93%

Application of ion beam sputtering for high‐resolution electron microscopy

Kanaya

Baba

Muranaka

et al. 1986

J. Elec. Microsc. Tech.

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Ion beam sputtering for high-resolution electron microscopy has basically provided miscellaneous operational features such as atomic shadowing, uncoated observation, and etching of biological specimens coupled with tungsten sputter coating and thinning of solid materials.Based on the power-potential law of Lindhard for ionic impact phenomena on metal surfaces, the universal yield-energy relationship has been derived. Thereby the sputtering deposition rate with reference to the sputtering removal rate was obtained as a function of sputtering yield, and the most important angular distribution of sputtering yield could be measured by using the hemicylindrical specimen stage.Evidence is presented to show that ion beam sputtering has become one of the most powerful tools for high-resolution electron microscopy.

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“…Attempts to apply ion-etching technique to biological materials have been made since 1968 (LEWIS, 1968;HODGES et al, 1972;FULKER et al, 1973;FRISCH et al, 1974). From that time on, 10-30kV were used by many investigators for the ion-etching and it often resulted in artificial cone structures which were far from reflecting the intrinsic structures.…”

Section: Discussionmentioning

confidence: 99%

Scanning Electron Microscopic Observation on Intracellular Structures of Ion-Etched Materials

Tánaka¹,

Iino²,

Naguro³

1976

Arch. Histol. Jap., Arch. Histol. Jpn

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Summary. Ion-etching technique on the cracked surface of biological material may give a plastic visualization of intracellular structures under the scanning electron microscope, because membraneous structures in the cell are generally etch-resistant and the cytoplasmic matrix is easily ion-etched.Mild ion-etching using low voltage was applied to the cells of the pancreas. Nuclear pores were clearly disclosed but were enlarged slightly in the process of etching. Endoplasmic reticulum with ribosomes, Golgi apparatus, mitochondria, some filamentous structures and crystalline inclusions (B cell granules) were also effectively disclosed by ionetching technique.It is necessary, however, to compare the etched specimens carefully with non-etched ones to determine whether given structures observed are intrinsic ones or artifacts caused by etching.

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Demonstration of an Internal Structure within the Red Blood Cell by Ion Etching and Scanning Electron Microscopy

Cited by 51 publications

References 6 publications

Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy

Ultrastructural examination of dentin using focused ion-beam cross-sectioning and transmission electron microscopy

Application of ion beam sputtering for high‐resolution electron microscopy

Scanning Electron Microscopic Observation on Intracellular Structures of Ion-Etched Materials

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