Demonstration of a dual-mode digital-holographic/light-field-fluorescence microscope for extant life searches

Serabyn, Eugene; Kim, Terry D; Liewer, K. M.; Lindensmith, C. A.; Wallace, J. Kent; Oborny, Nathan; Bedrossian, Manuel; Rider, Stephanie; Nadeau, Jay

doi:10.1109/aero47225.2020.9172331

Cited by 2 publications

(1 citation statement)

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“…Based on these requirements and the eventual goal of future space missions, we developed a volumetric microscopy suite that is capable of capturing high-resolution morphology, motility, and fluorescence of a liquid volume. The suite, called Extant Life Volumetric Imaging System (ELVIS), combines digital holographic microscopy (DHM) with fluorescence light field Frontiers in Astronomy and Space Sciences frontiersin.org microscopy (FLFM) in order to capture both high-resolution morphology and fluorescence synchronously (Serabyn et al, 2020;Kim et al, 2020a). These volumetric imaging techniques capture signals from an extended depth of field and allows for a rapid and compressed imaging of the sample volume.…”

Section: Introductionmentioning

confidence: 99%

A multi-modal volumetric microscope with automated sample handling for surveying microbial life in liquid samples

Kim

Oborny²,

Serabyn³

et al. 2022

Front. Astron. Space Sci.

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In the study of microbial life, microscopy plays a unique role due to its ability to detect ordered structure, motility, and fluorescence signals. As such it has also recently gained attention in the context of searching for extant life on distant Solar System bodies bearing liquid water. In this paper we introduce a multi-modal volumetric microscopy system for potential future spaceflight missions that combines digital holographic microscopy (DHM) and volume fluorescence imager (VFI), which are volumetric imaging methods that provide high-resolution, high-throughput examination of liquid samples. DHM provides information on the absorption, morphology, and motility of imaged objects without requiring the use of contrast agents. On the other hand, VFI based on light field microscopy focuses on the fluorescence signals from the sample to observe specific structures dyed with targeted contrast agents or providing unique autofluorescence signals. We also present an autonomous sample handling and data acquisition system to allow for an autonomous mission to distant planets or moons, or for autonomous use in bodies of water on Earth. The full system, named ELVIS, or Extant Life Volumetric Imaging System, is capable of autonomously surveying a liquid sample to extract morphology, motility, and fluorescence signals of extant microbial life.

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Section: Introductionmentioning

confidence: 99%