2001
DOI: 10.1105/tpc.13.4.781
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Demonstration in Yeast of the Function of BP-80, a Putative Plant Vacuolar Sorting Receptor

Abstract: BP-80, later renamed VSR PS-1 , is a putative receptor involved in sorting proteins such as proaleurain to the lytic vacuole, with its N-terminal domain recognizing the vacuolar sorting determinant. Although all VSR PS-1 characteristics and in vitro binding properties described so far favored its receptor function, this function remained to be demonstrated. Here, we used green fluorescent protein (GFP) as a reporter in a yeast mutant strain defective for its own vacuolar receptor, Vps10p. By expressing VSR PS-… Show more

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Cited by 71 publications
(79 citation statements)
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“…The vacuolar accumulation of msGFP resembles previous observations that GFP variants were targeted to the yeast vacuole [21,35]. Intriguingly, vacuolar targeting of GFP was shown to depend on the sorting receptor Vps10 [21].…”
Section: A Partial Deletion Of Vps10 Prevents Vacuolar Targeting Of Msupporting
confidence: 63%
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“…The vacuolar accumulation of msGFP resembles previous observations that GFP variants were targeted to the yeast vacuole [21,35]. Intriguingly, vacuolar targeting of GFP was shown to depend on the sorting receptor Vps10 [21].…”
Section: A Partial Deletion Of Vps10 Prevents Vacuolar Targeting Of Msupporting
confidence: 63%
“…Budding yeasts contain a vacuolar sorting receptor called Vps10 that recognizes the carboxypeptidase Y (CPY) precursor as well as certain misfolded proteins [15][16][17][18][19][20]. When msGFP was targeted to the secretory pathway in S. cerevisiae, some of the msGFP molecules reached the vacuole in a Vps10-dependent manner, confirming a report that the quality control function of Vps10 extends to folded GFP [21]. Building on a previous functional dissection of Vps10 [15], we found that deletion of a single domain of Vps10 prevented diversion of msGFP to the vacuole without causing missorting of CPY.…”
Section: Introductionsupporting
confidence: 53%
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“…However, BP-80 also is known to bind to the C-terminal propeptide of 2S albumin, which lacks the NPIR motif (Kirsch et al, 1996). BP-80 is a member of a family of membrane proteins termed vacuolar sorting receptors (VSRs; Paris et al, 1997;Shimada et al, 1997;Hadlington and Denecke 2000;Paris and Neuhaus, 2002), whose function has been confirmed both in vitro (Cao et al, 2000) and in vivo using a yeast expression system (Humair et al, 2001). Targeting determinants also have been identified for proteins sorted into the PSV (Frigerio et al, 1998;Neuhaus and Rogers, 1998).…”
Section: Introductionmentioning
confidence: 99%
“…The barley (Hordeum vulgare) aleurain (Rogers et al, 1985) and sweet potato (Ipomoea batatas) sporamin (Murakami et al, 1986) have a vacuolar targeting signal, Asn-Pro-IleArg (NPIR), in their NTPPs Nakamura, 1991, 1999;Chrispeels and Raikhel, 1992). The NPIR sequence is recognized by a vacuolar sorting receptor such as pea (Pisum sativum) BP-80 (Paris et al, 1997;Humair et al, 2001), pumpkin (Cucurbita maxima) PV72 (Shimada et al, 1997), and Arabidopsis AtELP (Ahmed et al, 2000). On the other hand, NTPPs of human cathepsin B (P07858) and cathepsin S (M90696) function as an autoinhibitory domain that covers the active site within the molecule (Turk et al, 1996;Maubach et al, 1997).…”
mentioning
confidence: 99%