Demonstration and identification of a biosynthetic intermediate between farnesyl PP and squalene in a higher plant

Heintz, R.; Benveniste, Pierre; Robinson, William H.; Coates, Robert M.

doi:10.1016/0006-291x(72)90517-7

Cited by 10 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[14][15][16] In contrast, DMAPP is synthesized directly along with IPP without IPI activity by 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase via the MEP pathway in plastids. 4,5,17) Thus, the contribution of plant IPI to DMAPP synthesis downstream of the MEP pathway was ambiguous.…”

Section: The Early Stage Of the Biosynthetic Pathway For Diterpenementioning

confidence: 99%

The Biosynthesis of Isoprenoids and the Mechanisms Regulating It in Plants

Okada

2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Section: The Early Stage Of the Biosynthetic Pathway For Diterpenementioning

confidence: 99%

The Biosynthesis of Isoprenoids and the Mechanisms Regulating It in Plants

Okada

2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…In plants, two distinct pathways produce IPP and DMAPP: the mevalonate (MVA) pathway and the methylerythritol phosphate (MEP) pathway (Lichtenthaler et al 1997). Various isoprenoids, including sesquiterpenes, triterpenes, sterols, brassinosteroids, and prenylquinones, are synthesized in the cytoplasm from IPP derived from the MVA pathway (Heintz et al 1972;Nes and Venkatramesh 1999;Lange and Ghassemian 2003). Many diterpenoids, including gibberellins, abscisic acids, carotenoids, and phytol chains of chlorophylls, are separately synthesized in plastids from IPP and DMAPP, both derived from the MEP pathway (Lichtenthaler 1999;Rohmer 1999).…”

Section: Introductionmentioning

confidence: 97%

Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice

et al. 2007

View full text Add to dashboard Cite

Diterpenoid phytoalexins such as momilactones and phytocassanes are produced via geranylgeranyl diphosphate in suspension-cultured rice cells after treatment with a chitin elicitor. We have previously shown that the production of diterpene hydrocarbons leading to phytoalexins and the expression of related biosynthetic genes are activated in suspension-cultured rice cells upon elicitor treatment. To better understand the elicitor-induced activation of phytoalexin biosynthesis, we conducted microarray analysis using suspension-cultured rice cells collected at various times after treatment with chitin elicitor. Hierarchical cluster analysis revealed two types of early-induced expression (EIE-1, EIE-2) nodes and a late-induced expression (LIE) node that includes genes involved in phytoalexins biosynthesis. The LIE node contains genes that may be responsible for the methylerythritol phosphate (MEP) pathway, a plastidic biosynthetic pathway for isopentenyl diphosphate, an early precursor of phytoalexins. The elicitor-induced expression of these putative MEP pathway genes was confirmed by quantitative reverse-transcription PCR. 1-Deoxy-D: -xylulose 5-phosphate synthase (DXS), 1-deoxy-D: -xylulose 5-phosphate reductoisomerase (DXR), and 4-(cytidine 5'-diphospho)-2-C-methyl-D: -erythritol synthase (CMS), which catalyze the first three committed steps in the MEP pathway, were further shown to have enzymatic activities that complement the growth of E. coli mutants disrupted in the corresponding genes. Application of ketoclomazone and fosmidomycin, inhibitors of DXS and DXR, respectively, repressed the accumulation of diterpene-type phytoalexins in suspension cells treated with chitin elicitor. These results suggest that activation of the MEP pathway is required to supply sufficient terpenoid precursors for the production of phytoalexins in infected rice plants.

show abstract

“…Oxidative demethylation reactions at C-4 and C-14 were described by Mulheirn and Ramm (1972); stoichiometric amounts of C0 2 are released (Olson et al, 1957;Gautschi and Bloch, 1957). Demethylation at C-14 is hindered by the 93, 19-cyclopropane ring (Grunwald, 1975); enzyme mediated opening of this ring was observed by Heintz et al, (1972).…”

Section: Biosynthesis Of Common Sterols In Higher Plantsmentioning

confidence: 99%

“…An intermediate compound (presqualene alcohol), previously obtained from rat liver and yeast, was identified in bramble tissue (Heintz et al, 1972).…”

Section: Introductionmentioning

confidence: 99%

Physiological aspects of steroids in pollen of Pinus elliotti Engelm /

Peter¹

1979

View full text Add to dashboard Cite

Biochemical Aspects of Sterols in Higher PlantsDimethyl sulphoxide (10 -4 M) increased the relative germination to 82%, further addition of pregnenolone, estradiol, estriol or corticosterone (each 10 -5 M) restored germination to almost optimum levels. Animal steroid hormones increased the conductance of the pollen media; however, there was no discernible relationship between increased germination and

show abstract

Demonstration and identification of a biosynthetic intermediate between farnesyl PP and squalene in a higher plant

Cited by 10 publications

References 11 publications

The Biosynthesis of Isoprenoids and the Mechanisms Regulating It in Plants

The Biosynthesis of Isoprenoids and the Mechanisms Regulating It in Plants

Elicitor induced activation of the methylerythritol phosphate pathway toward phytoalexins biosynthesis in rice

Physiological aspects of steroids in pollen of Pinus elliotti Engelm /

Contact Info

Product

Resources

About