Demonstrating approaches to chemically modify the surface of Ag nanoparticles in order to influence their cytotoxicity and biodistribution after single dose acute intravenous administration

Pang, Chengfang; Brunelli, Andrea; Zhu, Conghui; Hristozov, Danail; Liu, Ying; Semenzin, Elena; Wang, Wenwen; Tao, Wuqun; Liang, Jingnan; Marcomini, Antonio; Chen, Chunying; Zhao, Bin

doi:10.3109/17435390.2015.1024295

Cited by 37 publications

(36 citation statements)

References 59 publications

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“…Citrate-coated AgNPs have a higher affinity to proteins than PVP-coated AgNPs, suggesting they could absorb more proteins on their surface (Ahlberg et al, 2014). However, the amount of protein (serum albumin) absorbed on AgNPs was similar in citrate- and PVP-coated AgNPs (Pang et al, 2016). In addition, protein corona composition was similar for citrate- and PVP-coated AgNPs after AgNP incubation in cell culture medium supplemented with fetal bovine serum (Shannahan et al, 2013).…”

Section: Discussionmentioning

confidence: 97%

Particle coatings but not silver ions mediate genotoxicity of ingested silver nanoparticles in a mouse model

et al. 2017

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Incorporation of silver nanoparticles (AgNPs) in toothpaste, food containers, dietary supplements and other consumer products can result in oral exposure to AgNPs and/or silver ions (Ag+) released from the surface of AgNPs. To examine whether ingestion of AgNPs or Ag+ results in genotoxic damage and whether AgNP coatings modulate the effect, we exposed mice orally to 20 nm citrate-coated AgNPs, polyvinylpyrrolidone (PVP)-coated AgNPs, silver acetate or respective vehicles at a 4 mg/kg dose (equivalent to 800x the EPA reference dose for Ag) for 7 days. Genotoxicity was examined in the systemic circulation and bone marrow at 1, 7, and 14 days post-exposure. We found that citrate-coated AgNPs induced chromosomal damage in bone marrow and oxidative DNA damage and double strand breaks in peripheral blood. These damages persisted for at least 14 days after exposure termination. Because oxidative DNA damage and strand breaks are repaired rapidly, their presence after exposure cessation indicates that citrate-coated AgNPs persist in the body. In contrast, PVP-coated AgNPs and silver acetate did not induce DNA or chromosomal damage at any time point measured. To determine whether coating-dependent genotoxicity is related to different AgNP changes in the gastrointestinal tract, we examined AgNP behavior and fate in an in vitro gastrointestinal digestion model using UV-visible spectroscopy and DLS. Citrate-coated AgNPs were more susceptible to agglomeration than PVP-coated AgNPs in digestive juices with or without proteins. In summary, AgNPs but not Ag+ are genotoxic following oral ingestion. Nanoparticle coatings modulate gastrointestinal transformation and genotoxicity of AgNPs, where higher agglomeration of AgNPs in gastrointestinal juices is associated with higher genotoxicity in tissues. Since genotoxicity is a strong indicator of cancer risk, further long-term studies focusing on cancer are warranted.

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Section: Discussionmentioning

confidence: 97%

Particle coatings but not silver ions mediate genotoxicity of ingested silver nanoparticles in a mouse model

et al. 2017

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“…Some predictive models of nanoparticles uptake by cells and apoptosis were developed and a strategy for modeling was applied for various nanoparticles or modifications of their surfaces. (Epa et al, 2012;Pang et al, 2016;Zhang et al, 2012). A model to describe toxic potencies of 17…”

Section: Introductionmentioning

confidence: 91%

Predicting toxic potencies of metal oxide nanoparticles by means of nano-QSARs

Zhao

et al. 2016

Nanotoxicology

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“…Compared with 20-and 100-nm PVPcoated AgNPs and 110-nm citrate-coated AgNPs, 20-nm citrate-coated AgNPs induced the highest levels of oxidative stress and necrosis (10). Furthermore, particle coating (polyethylene glycol vs. branched polyethyleneimine) was also observed to modify toxicity of AgNPs in the Hep-a1c1c7 mouse liver cell line (11).…”

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confidence: 95%

Genetic determinants of susceptibility to silver nanoparticle‐induced acute lung inflammation in mice

et al. 2017

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Silver nanoparticles (AgNPs) are employed in a variety of consumer products; however, rodent studies indicate that AgNPs can cause lung inflammation and toxicity in a strain- and particle type-dependent manner, but mechanisms of susceptibility remain unclear. The aim of this study was to assess the variation in AgNP-induced lung inflammation and toxicity across multiple inbred mouse strains and to use genome-wide association (GWA) mapping to identify potential candidate susceptibility genes. Mice received doses of 0.25 mg/kg of either 20-nm citrate-coated AgNPs or citrate buffer using oropharyngeal aspiration. Neutrophils in bronchoalveolar lavage fluid (BALF) served as markers of inflammation. We found significant strain- and treatment-dependent variation in neutrophils in BALF. GWA mapping identified 10 significant single-nucleotide polymorphisms (false discovery rate, 15%) in 4 quantitative trait loci on mouse chromosomes 1, 4, 15, and 18, and (neural precursor cell expressed developmentally downregulated gene 4-like; chromosome 18), (anocatmin 6; chromosome 15), and (Ring finger protein 220; chromosome 4) were considered candidate genes. Quantitative RT-PCR revealed significant inverse associations between mRNA levels of these genes and neutrophil influx. ,, and are candidate susceptibility genes for AgNP-induced lung inflammation that warrant additional exploration in future studies.-Scoville, D. K., Botta, D., Galdanes, K., Schmuck, S. C., White, C. C., Stapleton, P. L., Bammler, T. K., MacDonald, J. W., Altemeier, W. A., Hernandez, M., Kleeberger, S. R., Chen, L.-C., Gordon, T., Kavanagh, T. J. Genetic determinants of susceptibility to silver nanoparticle-induced acute lung inflammation in mice.

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Demonstrating approaches to chemically modify the surface of Ag nanoparticles in order to influence their cytotoxicity and biodistribution after single dose acute intravenous administration

Cited by 37 publications

References 59 publications

Particle coatings but not silver ions mediate genotoxicity of ingested silver nanoparticles in a mouse model

Particle coatings but not silver ions mediate genotoxicity of ingested silver nanoparticles in a mouse model

Predicting toxic potencies of metal oxide nanoparticles by means of nano-QSARs

Genetic determinants of susceptibility to silver nanoparticle‐induced acute lung inflammation in mice

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