Demethylation of the RB1 promoter concomitant with reactivation of TET2 and TET3 impairs gastric carcinogenesis in K19-Wnt1/C2mE transgenic mice

PTEN Expression Was Significantly Associated with PD-L1 Score but Not with EBV Infection in Gastric Cancer

“…The relative PTEN and PD-L1 mRNA expression were analyzed using 2 −ΔΔCt method on real-time PCR (Light Cycler 480, Roche), and GAPDH was the internal control. 14 …”

Dietary polyphenols and their relationship to the modulation of non-communicable chronic diseases and epigenetic mechanisms: A mini-review

“… ↑ HAT4 ↑ BDNF levels, GP + Aquatic exercise resulted in amelioration of functional capacity and mobility in PD patients. ( Oliveira et al, 2020 ) Cancer Genistein + Sulforophane + sodium butyrate Breast cancer cell line (MDA-MB-231 and MCF-7) treated with Genistein (5 µM) + Sulforophane (15 µM) + sodium butyrate (2,5 µM) for 72 h ↓ DNMT3A, ↓ DNMT3B, ↓ HDAC1, ↓ HDAC6, ↓ HDAC11 ↓ EZH2, ↓ SUV39H1 ↓ KAT2A, ↓ KAT2B, ↓ EP300, ↓ CBP ↑ apoptosis, ↓ cell cycle ( Sharma & Tollefsbol, 2022 ) Curcumim Gastric cancer cell line (HGC-27, MGC-803, MKN-1 and SGC-7901) treated with 30 – 50 µM for 24 h / K19-Wnt1/C2mE transgenic mice (Gan) Six-week-old treated with curcumin (0.005 % (w/v)) for 3 times a week for 48 weeks ↓ DMNT1 ↑ RB1 ↓ cell proliferation, ↑ apoptosis, ↓ cell cycle, ↓ inflammation ( Cao et al, 2020 ) Quercetin Acute myeloid leukemia (HL60 and U937) treated with 50 – 75 µM for 72 h /Human xenograft acute myeloid leukemia (AML) models - female mice (NOD.CB17-Prkdcscid/J lineage) 8–11-week-old treated with quercetin (120 mg/kg every 4 days for 20 days ↓ DNMT1, ↓ DNMT3A, ↓ HDACs ↑ DAPK1, ↑ BCL2L1, ↑ BAX, ↑ APAF1, ↑ BNIP3L ↑ apoptosis ( Alvarez et al, 2018 ) Hippophae rhamnoide Polyphenols Colorectal (HCT116, HT29, and FHC) treated with 40–120 µM for 48 h /tumor xenograft model - BALB/c female nude mice (five weeks old) were treated with Hippophae rhamnoide extract (50 mg/kg/day for 21 days) ↑ miR −195-5p, ↑ miR − 497-5p, ↓ miR-1247-3p ↓ CCND1 , ↓ CCND2 ↓ CCND3 , ↓ CCNE1 ↓ BCL-2 , ↑CASP2 ↑ apoptosis, ↓ cell cycle ( Wu et al, 2021 ) Apigenin Breast cancer cell line (MDA-MB-231) treated with 40 µM for 96 h / tumor xenograft model - female athymic nude mice (BALB/c-nu) aged 6 weeks treated with apigenin (25 mg/kg/day for 8 weeks) ↓ HDAC, ↑ HAT ↓ CDK1, ↓ CCNA2, ↓ CCNB1, ↑ CDKN1A ↓ cell proliferation, ↓ tumor growth ( Tseng et al, 2016 ) Genistein Head and neck squamous cell carcinoma (HNSCC) - Phase II clinical trial – 39 patients (300 mg/day for 3 weeks) ...…”