Demethylation-Driven ligase chain reaction for simultaneously sensitive detection of m6A demethylase FTO and m1A demethylase ALKBH3 in breast cancer at Single-Molecule level

Accurate identification of 8-oxoguanine in RNA with single-nucleotide resolution using ligase-dependent qPCR

“…Ligation and enzyme-based approaches have been used to detect various RNA modifications, including N 6-methyladenosine (m 6 A), 25–33 pseudouridine (ψ), 34 N 1-methyladenosine (m 1 A) and 2′- O -methylation, 35,36 within synthetic RNAs, ribosomal RNAs (rRNAs), or long non-coding RNAs (lncRNAs). They have also been used to identify DNA modifications, such as 8-oxodG, within synthetic DNAs.…”