Demand Drivers and Price Supports for Bioethanol Use as Fuel in the United States: A Brief Review

Lu, Ning; Rice, Robert W.

doi:10.13073/0015-7473-60.2.126

Cited by 6 publications

(4 citation statements)

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“…Ethanol is likely to play an important role in the development of renewable fuel [ 28 ]. During the fermentation for ethanol production, glycerol was generally formatted as a byproduct to maintain osmotic stress and prevent water loss under hyperosmotic conditions [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

CRISPR-Cas9 Approach Constructed Engineered Saccharomyces cerevisiae with the Deletion of GPD2, FPS1, and ADH2 to Enhance the Production of Ethanol

Yang

Jiang

et al. 2022

JoF

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Bioethanol plays an important value in renewable liquid fuel. The excessive accumulation of glycerol and organic acids caused the decrease of ethanol content in the process of industrial ethanol production. In this study, the CRISPR-Cas9 approach was used to construct S. cerevisiae engineering strains by the deletion of GPD2, FPS1, and ADH2 for the improvement of ethanol production. RNA sequencing and transcriptome analysis were used to investigate the effect of gene deletion on gene expression. The results indicated that engineered S. cerevisiae SCGFA by the simultaneous deletion of GPD2, FPS1, and ADH2 produced 23.1 g/L ethanol, which increased by 0.18% in comparison with the wild-type strain with 50 g/L of glucose as substrate. SCGFA strain exhibited the ethanol conversion rate of 0.462 g per g of glucose. In addition, the contents of glycerol, lactic acid, acetic acid, and succinic acid in SCGFA decreased by 22.7, 12.7, 8.1, 19.9, and 20.7% compared with the wild-type strain, respectively. The up-regulated gene enrichment showed glycolysis, fatty acid, and carbon metabolism could affect the ethanol production of SCGFA according to the Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Therefore, the engineering strain SCGFA had great potential in the production of bioethanol.

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Section: Discussionmentioning

confidence: 99%

CRISPR-Cas9 Approach Constructed Engineered Saccharomyces cerevisiae with the Deletion of GPD2, FPS1, and ADH2 to Enhance the Production of Ethanol

Yang

Jiang

et al. 2022

JoF

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show abstract

“…Ethanol is likely to play an important role in the development of renewable fuel [21]. During the fermentation for ethanol production, glycerol was generally formatted as a byproduct to maintain osmotic stress and prevent water loss under hyperosmotic conditions [22].…”

Section: Discussionmentioning

confidence: 99%

Improvement of the Bioethanol Yield in Saccharomyces cerevisiae GPD2 Delta FPS1 Delta ADH2 Delta Constructed by the CRISPR-Cas9 Approach with the Decrease of By-Product Formation

Yang

Jiang

et al. 2022

Preprint

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Background: Bioethanol plays an important value in renewable liquid fuel. The inhibition of by-product formation would enhance the ethanol production of Saccharomyces cerevisiae. In fact, the excessive accumulation of glycerol and organic acids caused the decrease of ethanol content in the process of industrial ethanol production. Results: In this study, S. cerevisiae engineering strains were constructed using the CRISPR-Cas9 approach to delete GPD2, FPS1, and ADH2 to improve the yield of ethanol with the decrease of by-product contents. Engineered S. cerevisiae SCGFA by the GPD2, FPS1, and ADH2 deletion produced 23.1 g/L with 50 g/L of glucose as substrate. SCGFA strain exhibited the ethanol conversion rate of 0.462 g per g of glucose. In addition, the contents of glycerol, lactic acid, acetic acid, and succinic acid in SCGFA decreased by 22.7, 12.7, 8.1, 19.9, and 20.7% compared with the wild-type strain, respectively. Conclusions: The co-knockout of GPD2, FPS1, and ADH2 dramatically improved the ethanol yield of S. cerevisiae by the inhibition of glycerol release and the prevention of ethanol consumption. This study developed a new S. cerevisiae engineering strain SCGFA with high-level ethanol production after GPD2, FPS1, and ADH2 deletion. The engineering strain SCGFA could apply in ethanol production with less formation of by-products.

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“…Even though there are strong policies supporting the development and commercialization of advanced biofuel, many of these projects are stymied at the blend wall until more refineries are able overcome barriers moving their stalled projects to production and commercialization. Janssen et al (2013) Funding, technology Lang (2013 a, b) Technology based on low process yields and high production costs Lu (2010) Barriers to production are technology-based high production costs Cheng and Timilsina (2010) Project closures due to low oil prices below $100/barrel, global financial situation, changing government support policies, immature processing technology, production costs, economic hurdles, and no clear choice for best technology pathway Sims et al (2009) There are a number of technical processing barriers that need to be overcome before full potential production is possible Naik et al (2009) Suggested that technological process scaling was a major barrier to commercial biofuel production Zu and Pan (2009) The early adopters of lignocellulosic technology were expected to carry the perceived risk of investment of uncertain technology, and that feedstock represents half of total production costs Bohlmann (2006) The barriers of technology and recalcitrance are major economic and operational challenges Lynd et al (2005) Knowledge gaps from the broad barrier categories are not precise enough to fully aid in developing an industry and its needed infrastructure. Furthermore, 75% of advanced biofuel projects have been lost since inception by 2013 (Lang 2013a, b).…”

Section: Policies Impacting Biofuelsmentioning

confidence: 99%

Bioeconomy Survey Results Regarding Barriers to the United States Advanced Biofuel Industry

2017

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Although the 2005 Environmental Protection Act (EPAct) was enacted to bolster the emerging biofuel industry, 52% of advanced biofuel (AB) projects ended by 2015. However, there are no complete lists of internal and external barriers that can help to explain why these projects are failing. The goal of this study was to develop a list of barriers impeding advanced biofuel projects by conducting a survey of biofuel stakeholders. Based on a literature review and previous research, a list of 23 hypothesized internal and external barriers was elaborated. A survey was conducted to have industry stakeholders provide their perception on the list of hypothesized barriers. The perceptions of industry stakeholders were analyzed by dividing the sample in three different stakeholder groups: advanced biofuel industry members, government representatives, and a third category called others that included publishers, journalists, suppliers, and other related stakeholders to the industry. In addition, nonparametric statistical techniques were used to compare the perceptions of the groups. The most significant results indicated that Technology issues was considered as an internal barrier for the three groups while Funding and Renewable Fuel Standards were perceived as external barriers by the three groups too. In addition, the rating of barriers was further analyzed only by AB industry stakeholders in order to uncover more details on the perception of barriers that might be preventing the AB industry to prosper.

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Demand Drivers and Price Supports for Bioethanol Use as Fuel in the United States: A Brief Review

Cited by 6 publications

References 0 publications

CRISPR-Cas9 Approach Constructed Engineered Saccharomyces cerevisiae with the Deletion of GPD2, FPS1, and ADH2 to Enhance the Production of Ethanol

CRISPR-Cas9 Approach Constructed Engineered Saccharomyces cerevisiae with the Deletion of GPD2, FPS1, and ADH2 to Enhance the Production of Ethanol

Improvement of the Bioethanol Yield in Saccharomyces cerevisiae GPD2 Delta FPS1 Delta ADH2 Delta Constructed by the CRISPR-Cas9 Approach with the Decrease of By-Product Formation

Bioeconomy Survey Results Regarding Barriers to the United States Advanced Biofuel Industry

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