Deltamethrin Resistance Mechanisms in Aedes aegypti Populations from Three French Overseas Territories Worldwide

Dusfour, Isabelle; Zorrilla, Pilar; Guidez, Amandine; Issaly, Jean; Girod, Romain; Guillaumot, Laurent; Robello, Carlos; Strode, Clare

doi:10.1371/journal.pntd.0004226

Cited by 83 publications

(115 citation statements)

References 46 publications

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“…In this study, we find that the miR-932-mimic-injected mosquitoes become more resistant to deltamethrin, while the miR-932-inhibitor-injected mosquitoes become more susceptible to deltamethrin, suggesting that miR-932 participates in the regulation of pyrethroid resistance. The role of miR-932 in pyrethroid resistance also offers new ideas about exploring the mechanisms of insecticide resistance, which would help to make more pertinent measures in controlling mosquito populations (Dusfour et al 2015). All the way through, there are two main molecular mechanisms that can elucidate insecticide resistance, one is target site modification represented by knock-down effect, another is metabolic detoxification, such as the cytochrome P450 monooxygenases (Itokawa et al 2015, Vera-Maloof et al 2015.…”

Section: Discussionmentioning

confidence: 99%

MiR-932 Regulates Pyrethroid Resistance inCulex pipiens pallens(Diptera: Culicidae)

Liu¹,

Tian²,

Guo³

et al. 2016

J Med Entomol

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MicroRNAs (miRNAs) play notable role in regulation of gene expression at the posttranscription level, and have been involved in many biological processes, including insecticide resistance. In this study, we investigated the role of miR-932 in the molecular mechanisms of pyrethroid resistance in Culex pipiens pallens (L.). Overexpression of miR-932 in the DS-strain made the mosquitoes more resistant to deltamethrin, while inhibiting the expression of miR-932 in the DR-strain made the mosquitoes more sensitive to deltamethrin. Further, we also identified CpCPR5 as a target gene of miR-932. Sustained overexpression of miR-932 resulted in repression of CpCPR5, and that knockdown of miR-932 increased CpCPR5 expression. In addition, knockdown of CpCPR5 decreased the sensitivity of mosquitoes to deltamethrin in the DS-strain. In conclusion, our study finds a molecular link between miR-932 and CpCPR5 and provides a novel insight into the mechanism of insecticide resistance.Key words: Culex pipiens pallens, pyrethroid, microRNA, CpCPR5Mosquitoes transmit a lot of pathogens, causing severe diseases such as malaria, dengue fever, West Nile fever, encephalitis, and filariasis, which threat public health worldwide (Andriessen et al. 2015, Fortuna et al. 2015, Paredes-Esquivel et al. 2015. Recent estimates show that there are about 400 million infections of dengue fever annually around the world (Sood et al. 2015). According to the WHO world malaria report, there are still 214 million new infections worldwide in 2015. Therefore, it is imperative for controlling and preventing mosquito-borne diseases. However, due to the lack of effective vaccines and drugs, vector control mostly relies on insecticides to reduce the number of mosquitoes (Glunt et al. 2015). But continuous and lasting application of insecticides has led to a constant increasing resistance, which makes antivector strategies more challenging (Rasic et al. 2015). Insecticide resistance arises from complex polygenic inheritance and many genes involved , Bonizzoni et al. 2015. These genes can be regulated at transcriptional and posttranscriptional levels and microRNA (miRNA) is known to be a posttranscriptional gene regulator (Wang et al. 2016).MiRNA is a class of small noncoding RNA, with about 22 nucleotides in length. It has been shown to repress translation via imperfectly pairing with complementary sites within the 3'UTR of target mRNA (Liu et al. 2014, Munoz et al. 2015. In diverse cellular programs, miRNA plays a key role and involves in cell growth, differentiation, apoptosis, and many other physiological processes , Park et al. 2015. In insects, the studies on miRNAs are mostly focused on the development, behavior, and hostpathogen interactions (Lucas et al. 2015). Recent evidence has shown that a few miRNAs participate in the regulation of insecticide resistance in mosquitoes, but the exact role of miRNA in pyrethroid resistance is still under little attention yet .Here, according to our previous study, we identified many different expressed miRNAs...

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Section: Discussionmentioning

confidence: 99%

MiR-932 Regulates Pyrethroid Resistance inCulex pipiens pallens(Diptera: Culicidae)

Liu¹,

Tian²,

Guo³

et al. 2016

J Med Entomol

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“…However, the V1016G mutation has not been reported in Latin America [31,32,46,47]. Instead, mutation V1016I was found, and often coexists with F1534C in South and North America, such as Venezuela [48], three French overseas territories [49], Brazil [50,51,52], Mexico [53], and the USA [54]. More recently, V1016I and F1534C were detected in Ghana, Africa [55], and a new mutation, T1520I, was found along with the F1534C mutation in a population in India [56].…”

Section: Identification Of Single Nucleotide Polymorphisms (Snps)mentioning

confidence: 99%

“…So far, V1016G has been detected only in Southeast Asia [34,37,40,41,44,45], whereas V1016I is distributed in South and North America [48,49,50,51,52,53]. More recently, V1016G, along with F1534C, was also detected in Ghana.…”

Section: Evolution Of Kdr Mutations In Aedes Aegyptimentioning

confidence: 99%

Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

Nomura

Zhorov

et al. 2016

Insects

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142

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Pyrethroid insecticides are widely used to control insect pests and human disease vectors. Voltage-gated sodium channels are the primary targets of pyrethroid insecticides. Mutations in the sodium channel have been shown to be responsible for pyrethroid resistance, known as knockdown resistance (kdr), in various insects including mosquitoes. In Aedes aegypti mosquitoes, the principal urban vectors of dengue, zika, and yellow fever viruses, multiple single nucleotide polymorphisms in the sodium channel gene have been found in pyrethroid-resistant populations and some of them have been functionally confirmed to be responsible for kdr in an in vitro expression system, Xenopus oocytes. This mini-review aims to provide an update on the identification and functional characterization of pyrethroid resistance-associated sodium channel mutations from Aedes aegypti. The collection of kdr mutations not only helped us develop molecular markers for resistance monitoring, but also provided valuable information for computational molecular modeling of pyrethroid receptor sites on the sodium channel.

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“…aegypti and Ae. albopictus showed that most of detoxification enzymes linked to insecticide resistance belong to the cytochrome P450 genes [23, 24]. Target insensitivity is due to mutations that reduce the binding affinity between the insecticide and its physiological target.…”

Section: Introductionmentioning

confidence: 99%

“…In many mosquito species, including Ae. aegypti , kdr related to pyrethroids and DDT has been located in segment 6 of domain II [23, 29–31], with other mutations at the same position ( I1011M , I1011V , V1016G and V1016I ) [32]. In South-East Asia, for example, two major voltage-gated sodium channel haplotypes ( S989P + V1016G and F1534C ) confer resistance of Ae.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility profile and metabolic mechanisms involved in Aedes aegypti and Aedes albopictus resistant to DDT and deltamethrin in the Central African Republic

et al. 2016

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Background Aedes aegypti and Ae. albopictus are the main epidemic vectors of dengue, chikungunya and Zika viruses worldwide. Their control during epidemics relies mainly on control of larvae and adults with insecticides. Unfortunately, loss of susceptibility of both species to several insecticide classes limits the efficacy of interventions. In Africa, where Aedes-borne viruses are of growing concern, few data are available on resistance to insecticides. To fill this gap, we assessed the susceptibility to insecticides of Ae. aegypti and Ae. albopictus populations in the Central African Republic (CAR) and studied the mechanisms of resistance.MethodsImmature stages were sampled between June and September 2014 in six locations in Bangui (the capital of CAR) for larval and adult bioassays according to WHO standard procedures. We also characterized DDT- and pyrethroid-resistant mosquitoes molecularly and biochemically, including tests for the activities of nonspecific esterases (α and β), mixed-function oxidases, insensitive acetylcholinesterase and glutathione S-transferases.ResultsLarval bioassays, carried out to determine the lethal concentrations (LC50 and LC95) and resistance ratios (RR50 and RR95), suggested that both vector species were susceptible to Bacillus thuringiensis var. israeliensis and to temephos. Bioassays of adults showed susceptibility to propoxur and fenitrothion, except for one Ae. albopictus population that was suspected to be resistant to fenithrothion. None of the Ae. aegypti populations was fully susceptible to DDT. Ae. albopictus presented a similar profile to Ae. aegypti but with a lower mortality rate (41%). Possible resistance to deltamethrin was observed among Ae. aegypti and Ae. albopictus, although some were susceptible. No kdr mutations were detected in either species; however, the activity of detoxifying enzymes was higher in most populations than in the susceptible Ae. aegypti strain, confirming decreased susceptibility to DDT and deltamethrin.ConclusionThese findings suggested that regular, continuous monitoring of resistance is necessary in order to select the most effective adulticides for arbovirus control in Bangui.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1887-5) contains supplementary material, which is available to authorized users.

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Deltamethrin Resistance Mechanisms in Aedes aegypti Populations from Three French Overseas Territories Worldwide

Cited by 83 publications

References 46 publications

MiR-932 Regulates Pyrethroid Resistance inCulex pipiens pallens(Diptera: Culicidae)

MiR-932 Regulates Pyrethroid Resistance inCulex pipiens pallens(Diptera: Culicidae)

Sodium Channel Mutations and Pyrethroid Resistance in Aedes aegypti

Susceptibility profile and metabolic mechanisms involved in Aedes aegypti and Aedes albopictus resistant to DDT and deltamethrin in the Central African Republic

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