delta1-piperideine-2-carboxylate reductase of Pseudomonas putida

Abstract

Pseudomonas putida metabolizes D-lysine to Al-piperideine-2-carboxylate and L-pipecolate. The second step of this catabolic pathway is catalyzed by A'piperideine-2-carboxylate reductase. This enzyme was isolated and purified from cells grown on DL-lysine as substrate. The enzyme was very unstable, resulting in low recovery of activity and low purity after a six-step purification procedure. The enzyme had a pH optimum of 8.0 to 8.3. The Km values for A-piperideine-2carboxylate and NADPH were 0.23 and 0.13 mM, respectively. NADPH at concentrations above 0.15 mM was inhibitory to the enzyme. A1-pyrroline-5carboxylate, pyroglutamate, and NADH were poor substrates or coenzyme for Al-piperideine-2-carboxylate reductase. The enzyme reaction from A-piperideine-2-carboxylate to L-pipecolate was irreversible. EDTA, sodium pyrophosphate, and dithiothreitol at concentrations of 1 mM protected the enzyme during storage. The enzyme was inhibited almost totally by Zn2+, Mn2i, Hg2+ Co2+,

Mammalian forebrain ketimine reductase identified as μ‐crystallin; potential regulation by thyroid hormones

“…They include the previously partially characterised Δ 1 ‐piperideine‐2‐carboxylate (P2C)/Δ 1 ‐pyrroline‐2‐carboxylate (Pyr2C) reductase (E.C. 1.5.1.21) isolated from porcine kidney (Petrakis and Greenberg 1965) and Pseudomonas putida (Payton and Chang 1982), and ketimine reductase (E.C. 1.5.1.25) isolated from bovine cerebellum (Nardini et al.…”

Lysine biosynthesis in Rhodotorula glutinis: properties of pipecolic acid oxidase

“…However, the biological significance and the properties of the Pseudomonas enzyme are completely different from those of the R. glutinis enzyme. The Pseudomonas enzyme is used in the catabolism of lysine (2,14), whereas the R. glutinis enzyme is used for the biosynthesis of lysine and not in its catabolism (J. J. Kinzel, Ph.D. thesis, submitted to Miami University, Oxford, Ohio). The Pseudomonas enzyme was associated with an electron transport particle.…”

“…Pipecolic acid is used as a carbon and nitrogen source by Pseudomonas spp. (14). For this catabolism, pipecolic acid is converted to ASA by pipecolic acid oxidase.…”