Delta-like 1 homolog (DLK1) inhibits proliferation and myotube formation of avian QM7 myoblasts

Shin, Sangsu; Choi, Young Min; Suh, Young Ho; Lee, Kichoon

doi:10.1016/j.cbpb.2014.09.006

Cited by 10 publications

(9 citation statements)

References 33 publications

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“…Interestingly, in Runx1 PM and mdx muscles, we found that Delta- like homolog 1 ( Dlk1 ), a putative Delta- Notch antagonist [ 38 ], to be significantly upregulated. As it was previously shown that Dlk1 inhibits proliferation in avian [ 39 ] and mouse [ 40 , 41 ] myoblasts it is tempting to speculate that Runx1 participates in the myoblast Delta-Notch signaling pathway by repressing the antagonist Dlk1 thereby promoting Delta-mediated myoblast proliferation. In case of the Igf-1/Akt/mTor pathway, which regulates muscle hypertrophy [ 42 ] and SC proliferation and differentiation [ 43 ], we found the two isoforms of Igf-1 downstream mediator protein kinase C (PKCβ and PKCδ) among the in vivo high-confidence gene subset.…”

Section: Discussionmentioning

confidence: 99%

Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration

et al. 2015

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Following myonecrosis, muscle satellite cells proliferate, differentiate and fuse, creating new myofibers. The Runx1 transcription factor is not expressed in naïve developing muscle or in adult muscle tissue. However, it is highly expressed in muscles exposed to myopathic damage yet, the role of Runx1 in muscle regeneration is completely unknown. Our study of Runx1 function in the muscle’s response to myonecrosis reveals that this transcription factor is activated and cooperates with the MyoD and AP-1/c-Jun transcription factors to drive the transcription program of muscle regeneration. Mice lacking dystrophin and muscle Runx1 (mdx - /Runx1 f/f), exhibit impaired muscle regeneration leading to age-dependent muscle waste, gradual decrease in motor capabilities and a shortened lifespan. Runx1-deficient primary myoblasts are arrested at cell cycle G1 and consequently differentiate. Such premature differentiation disrupts the myoblasts’ normal proliferation/differentiation balance, reduces the number and size of regenerating myofibers and impairs muscle regeneration. Our combined Runx1-dependent gene expression, ChIP-seq, ATAC-seq and histone H3K4me1/H3K27ac modification analyses revealed a subset of Runx1-regulated genes that are co-occupied by MyoD and c-Jun in mdx - /Runx1 f/f muscle. The data provide unique insights into the transcriptional program driving muscle regeneration and implicate Runx1 as an important participant in the pathology of muscle wasting diseases.

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Section: Discussionmentioning

confidence: 99%

Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration

et al. 2015

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“…However, increased muscle mass obtained through intense genetic selection and feeding regimes have resulted in concomitant changes in the characteristics of muscle fibers, which are the major components of skeletal muscle ( Choi et al , 2013a ; Choi et al , 2014 ; Picard et al , 2006 ; Rehfeldt et al , 2008 ; Shin et al , 2015 ). Generally, animals raised for meat production, such as quail, pigs, and cattle, weigh more and have muscles with a higher percentage of the larger type IIB fibers (fast-twitch and glycolytic) ( Choi and Kim, 2009 ; Choi et al , 2013b ; Remignon et al , 1995 ; Rehfeldt et al , 2000 ).…”

Section: Introductionmentioning

confidence: 99%

Carcass Performance, Muscle Fiber, Meat Quality, and Sensory Quality Characteristics of Crossbred Pigs with Different Live Weights

Choi

2016

Korean Journal for Food Science of Animal Resources

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In order to attain heavier live weight without impairing pork or sensory quality characteristics, carcass performance, muscle fiber, pork quality, and sensory quality characteristics were compared among the heavy weight (HW, average live weight of 130.5 kg), medium weight (MW, average weight of 111.1 kg), and light weight (LW, average weight of 96.3 kg) pigs at time of slaughter. The loin eye area was 1.47 times greater in the HW group compared to the LW group (64.0 and 43.5 cm2, p<0.001), while carcass percent was similar between the HW and MW groups (p>0.05). This greater performance by the HW group compared to the LW group can be explained by a greater total number (1,436 vs. 1,188, ×103, p<0.001) and larger area (4,452 vs. 3,716 μm2, p<0.001) of muscle fibers. No significant differences were observed in muscle pH45 min, lightness, drip loss, and shear force among the groups (p>0.05), and higher live weights did not influence sensory quality attributes, including tenderness, juiciness, and flavor. Therefore, these findings indicate that increased live weights in this study did not influence the technological and sensory quality characteristics. Moreover, muscles with a higher number of medium or large size fibers tend to exhibit good carcass performance without impairing meat and sensory quality characteristics.

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“…There are many studies investigating how genetic selection influences muscle fiber and meat quality characteristics in chicken and turkey (Fowler et al, 1980; Cherel et al, 1994; Remignon et al, 1995; Velleman et al, 2002; 2003). However, there are only a few studies conducted on meat quality characteristics between the Japanese quail lines, even though there are many reports regarding the effects of genetic selection on ultimate muscle mass and fiber characteristics (Narinc et al, 2013; Choi et al, 2014a; Shin et al, 2015). Therefore, the objective of this study was to compare the characteristics of the pectoralis major muscle, muscle fiber, and meat quality characteristics between the HW and RBC quail lines to better understand the effects of selection for production efficiency of quail.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Muscle Fiber and Meat Quality Characteristics in Different Japanese Quail Lines

Choi

Hwang

Lee

2016

Asian Australas. J. Anim. Sci

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The aim of this study was to compare the growth performance, fiber characteristics of the pectoralis major muscle, and meat quality characteristics in the heavy weight (HW) and random bred control (RBC) quail lines and genders. The HW male exhibited more than two times greater body (245.7 vs 96.1 g, p<0.05) and pectoralis major muscle (PMW; 37.1 vs 11.1 g, p<0.05) weights compared to the RBC female. This growth performance in the HW line was associated with a greater muscle fiber area (1,502 vs 663.0 μm2, p<0.001) compared to the RBC line. Greater muscle mass of the HW male was accompanied by a higher percentage of type IIB fiber compared to the HW female (64.0% vs 51.0%, p<0.05). However, muscle fiber hyperplasia (increase in fiber number) has had a somewhat limited effect on PMW between the two lines. On the other hand, the HW line harboring a higher proportion of type IIB fiber showed rapid pH decline at the early postmortem period (6.23 vs 6.41, p<0.05) and lighter meat surface (53.5 vs 47.3, p<0.05) compared to the RBC line harboring a lower proportion of type IIB fiber. There were no significant differences observed in the measurement of water-holding capacity including drip loss (2.74% vs 3.07%, p>0.05) and cooking loss (21.9% vs 20.4%, p>0.05) between the HW and RBC lines. Therefore, the HW quail line developed by selection from the RBC quail, was slightly different in the meat quality characteristics compared to the RBC line, and a marked difference was found in growth performance between the two quail lines.

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Delta-like 1 homolog (DLK1) inhibits proliferation and myotube formation of avian QM7 myoblasts

Cited by 10 publications

References 33 publications

Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration

Runx1 Transcription Factor Is Required for Myoblasts Proliferation during Muscle Regeneration

Carcass Performance, Muscle Fiber, Meat Quality, and Sensory Quality Characteristics of Crossbred Pigs with Different Live Weights

Comparison of Muscle Fiber and Meat Quality Characteristics in Different Japanese Quail Lines

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